ERCC1 Antibody - #AF0154

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产品: ERCC1 抗体
货号: AF0154
描述: Rabbit polyclonal antibody to ERCC1
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
分子量: 36kDa; 33kD(Calculated).
蛋白号: P07992
RRID: AB_2833335

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(90%), Dog(100%), Xenopus(88%)
克隆:
Polyclonal
特异性:
ERCC1 Antibody detects endogenous levels of total ERCC1.
RRID:
AB_2833335
引用格式: Affinity Biosciences Cat# AF0154, RRID:AB_2833335.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

COFS 4; COFS4; DNA excision repair protein ERCC 1; DNA excision repair protein ERCC-1; DNA excision repair protein ERCC1; ERCC 1; ERCC1; ERCC1_HUMAN; Excision repair cross complementation group 1; Excision repair cross complementing 1; Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1; Excision repair protein; RAD 10; RAD10; UV 20; UV20;

抗原和靶标

免疫原:
Uniprot:

P07992(ERCC1_HUMAN) >>Visit HPA database.

基因/基因ID:
ERCC1(2067)
描述:
ERCC1 a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA excision repair. Belongs to the ERCC1/RAD10/SWI10 family. Heterodimer composed of ERCC1 and XPF/ERRC4. Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4, a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord.
序列:
MDPGKDKEGVPQPSGPPARKKFVIPLDEDEVPPGVAKPLFRSTQSLPTVDTSAQAAPQTYAEYAISQPLEGAGATCPTGSEPLAGETPNQALKPGAKSNSIIVSPRQRGNPVLKFVRNVPWEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLMEKLEQDFVSRVTECLTTVKSVNKTDSQTLLTTFGSLEQLIAASREDLALCPGLGPQKARRLFDVLHEPFLKVP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Sheep
90
Xenopus
88
Zebrafish
80
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07992 作为底物

Site PTM Type Enzyme
Ubiquitination
M1 Acetylation
S14 Phosphorylation
K21 Ubiquitination
K37 Acetylation
K37 Ubiquitination
S104 Phosphorylation
K114 Ubiquitination
K162 Ubiquitination
K213 Ubiquitination
K226 Ubiquitination
K243 Ubiquitination
K247 Ubiquitination
K281 Ubiquitination
K295 Acetylation
K295 Ubiquitination

研究背景

功能:

Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.

细胞定位:

Nucleus.

Cytoplasm. Nucleus.

Nucleus.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterodimer composed of ERCC1 isoform 1 and XPF/ERRC4.

蛋白家族:

Belongs to the ERCC1/RAD10/SWI10 family.

研究领域

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

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