NEDD8 Antibody - #BF0274

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产品: NEDD8 抗体
货号: BF0274
描述: Mouse monoclonal antibody to NEDD8
应用: WB IHC IF/ICC ELISA FACS
反应: Human, Monkey
分子量: 9kDa; 9kD(Calculated).
蛋白号: Q15843
RRID: AB_2833981

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 50ul RMB¥ 1800 现货
 100ul RMB¥ 2800 现货

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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Mouse
应用:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Monkey
克隆:
Monoclonal [AFB1958]
特异性:
NEDD8 antibody detects endogenous levels of total NEDD8.
RRID:
AB_2833981
引用格式: Affinity Biosciences Cat# BF0274, RRID:AB_2833981.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ43224; MGC104393; MGC125896; MGC125897; NED8; NEDD 8; NEDD-8; Nedd8; NEDD8_HUMAN; Neddylin; Neural precursor cell expressed developmentally down regulated 8; Neural precursor cell expressed developmentally down regulated gene 8; Neural precursor cell expressed developmentally down-regulated protein 8; Rub1; Ubiquitin like protein Nedd 8; Ubiquitin like protein Nedd8; Ubiquitin-like protein Nedd8;

抗原和靶标

免疫原:

Purified recombinant fragment of human NEDD8 expressed in E. Coli.

Uniprot:

Q15843(NEDD8_HUMAN) >>Visit HPA database.

基因/基因ID:
NEDD8(4738)
表达:
Q15843 NEDD8_HUMAN:

Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

描述:
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
序列:
MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLRQ

翻译修饰 - Q15843 作为底物

Site PTM Type Enzyme
K4 Ubiquitination
K6 Ubiquitination
T7 Phosphorylation
T9 Phosphorylation
K11 Acetylation
K11 Sumoylation
K11 Ubiquitination
T20 Phosphorylation
K22 Ubiquitination
K27 Ubiquitination
K33 Ubiquitination
Y45 Phosphorylation
S46 Phosphorylation
K48 Acetylation
K48 Sumoylation
K48 Ubiquitination
K54 Acetylation
K54 Ubiquitination
Y59 Phosphorylation
K60 Ubiquitination
S65 Phosphorylation

研究背景

功能:

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

翻译修饰:

Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.

细胞定位:

Nucleus.
Note: Mainly nuclear.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

亚基结构:

Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53.

蛋白家族:

Belongs to the ubiquitin family.

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