产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0117, RRID:AB_2833978.
展开/折叠
150kDa medium; 160 kDa neurofilament protein; 2 kDa neurofilament protein; 200 kDa neurofilament protein; 68 kDa neurofilament protein; AI847934; CMT1F; CMT2CC; CMT2E; FLJ53642; KIAA0845; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; mKIAA0845; Nef3; NEFH; NEFL; NEFM; Neurofilament 3; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament light polypeptide 68kDa; Neurofilament light polypeptide; Neurofilament medium polypeptide 150kDa; Neurofilament medium polypeptide; Neurofilament protein heavy polypeptide; Neurofilament protein light chain; Neurofilament protein light polypeptide; Neurofilament protein medium polypeptide; neurofilament subunit NF-L; Neurofilament triplet H protein; Neurofilament triplet L protein; Neurofilament triplet M protein; neurofilament-3 (150 kD medium); NF-H; NF-L; NF-M; NF160; NF165; NF200; NF68; NFH; NFL; NFM; NFM_HUMAN; PPP1R110; protein phosphatase 1, regulatory subunit 110;
抗原和靶标
Purified recombinant fragment of human NEFL expressed in E. Coli.
- P07196 NFL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD
翻译修饰 - P07196 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| Y14 | Phosphorylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| T21 | Phosphorylation | Q00535 (CDK5) , P28482 (MAPK1) | Uniprot |
| R23 | Methylation | Uniprot | |
| R30 | Methylation | Uniprot | |
| R37 | Methylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K362 | Acetylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| Y372 | Phosphorylation | Uniprot | |
| K379 | Acetylation | Uniprot | |
| Y389 | Phosphorylation | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| T520 | Phosphorylation | Uniprot |
研究背景
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
O-glycosylated.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Ubiquitinated in the presence of TRIM2 and UBE2D1.
Interacts with ARHGEF28. Interacts with TRIM2.
The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
Belongs to the intermediate filament family.
研究领域
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
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