NEFL Antibody - #BF0117

(2)  
产品: NEFL 抗体
货号: BF0117
描述: Mouse monoclonal antibody to NEFL
应用: WB IHC IF/ICC ELISA FACS
反应: Human
分子量: 62kDa; 62kD(Calculated).
蛋白号: P07196
RRID: AB_2833978

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   规格 价格 库存
 50ul RMB¥ 1800 现货
 100ul RMB¥ 2800 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse
应用:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFB1955]
特异性:
NEFL antibody detects endogenous levels of total NEFL.
RRID:
AB_2833978
引用格式: Affinity Biosciences Cat# BF0117, RRID:AB_2833978.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

150kDa medium; 160 kDa neurofilament protein; 2 kDa neurofilament protein; 200 kDa neurofilament protein; 68 kDa neurofilament protein; AI847934; CMT1F; CMT2CC; CMT2E; FLJ53642; KIAA0845; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; mKIAA0845; Nef3; NEFH; NEFL; NEFM; Neurofilament 3; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament light polypeptide 68kDa; Neurofilament light polypeptide; Neurofilament medium polypeptide 150kDa; Neurofilament medium polypeptide; Neurofilament protein heavy polypeptide; Neurofilament protein light chain; Neurofilament protein light polypeptide; Neurofilament protein medium polypeptide; neurofilament subunit NF-L; Neurofilament triplet H protein; Neurofilament triplet L protein; Neurofilament triplet M protein; neurofilament-3 (150 kD medium); NF-H; NF-L; NF-M; NF160; NF165; NF200; NF68; NFH; NFL; NFM; NFM_HUMAN; PPP1R110; protein phosphatase 1, regulatory subunit 110;

抗原和靶标

免疫原:

Purified recombinant fragment of human NEFL expressed in E. Coli.

Uniprot:

P07196(NFL_HUMAN) >>Visit HPA database.

基因/基因ID:
NEFL(4747)
描述:
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
序列:
MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD

翻译修饰 - P07196 作为底物

Site PTM Type Enzyme
S2 Acetylation
S3 Phosphorylation
Y14 Phosphorylation
K15 Ubiquitination
T21 Phosphorylation Q00535 (CDK5) , P28482 (MAPK1)
R23 Methylation
R30 Methylation
R37 Methylation
S56 Phosphorylation
S103 Phosphorylation
T154 Phosphorylation
K157 Ubiquitination
S215 Phosphorylation
S221 Phosphorylation
K271 Ubiquitination
K362 Acetylation
K370 Ubiquitination
Y372 Phosphorylation
K379 Acetylation
Y389 Phosphorylation
S472 Phosphorylation
T520 Phosphorylation

研究背景

功能:

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

翻译修饰:

O-glycosylated.

Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

Ubiquitinated in the presence of TRIM2 and UBE2D1.

亚基结构:

Interacts with ARHGEF28. Interacts with TRIM2.

蛋白家族:

The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.

Belongs to the intermediate filament family.

研究领域

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

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