产品: GPR84 抗体
货号: DF2769
描述: Rabbit polyclonal antibody to GPR84
应用: WB IHC
文献验证: IHC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 38 kDa; 44kD(Calculated).
蛋白号: Q9NQS5
RRID: AB_2839975

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 50ul RMB¥ 1250 现货
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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
GPR84 Antibody detects endogenous levels of total GPR84.
RRID:
AB_2839975
引用格式: Affinity Biosciences Cat# DF2769, RRID:AB_2839975.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EX 33; EX33; G protein coupled receptor 84; G protein coupled receptor EX 33; G protein coupled receptor EX33; G-protein coupled receptor 84; GPCR 4; GPCR4; GPR 84; GPR84; GPR84_HUMAN; Inflammation related G protein coupled receptor EX 33; Inflammation related G protein coupled receptor EX33; Inflammation-related G-protein coupled receptor EX33;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9NQS5 GPR84_HUMAN:

Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta and intestine. Within the leukocyte population expression is higher in neutrophils and eosinophils relative to T- or B-lymphocytes.

描述:
Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.
序列:
MWNSSDANFSCYHESVLGYRYVAVSWGVVVAVTGTVGNVLTLLALAIQPKLRTRFNLLIANLTLADLLYCTLLQPFSVDTYLHLHWRTGATFCRVFGLLLFASNSVSILTLCLIALGRYLLIAHPKLFPQVFSAKGIVLALVSTWVVGVASFAPLWPIYILVPVVCTCSFDRIRGRPYTTILMGIYFVLGLSSVGIFYCLIHRQVKRAAQALDQYKLRQASIHSNHVARTDEAMPGRFQELDSRLASGGPSEGISSEPVSAATTQTLEGDSSEVGDQINSKRAKQMAEKSPPEASAKAQPIKGARRAPDSSSEFGKVTRMCFAVFLCFALSYIPFLLLNILDARVQAPRVVHMLAANLTWLNGCINPVLYAAMNRQFRQAYGSILKRGPRSFHRLH

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Sheep
89
Rabbit
89
Xenopus
67
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta and intestine. Within the leukocyte population expression is higher in neutrophils and eosinophils relative to T- or B-lymphocytes.

蛋白家族:

Belongs to the G-protein coupled receptor 1 family.

文献引用

1). Novel macrophage-related gene prognostic index for glioblastoma associated with M2 macrophages and T cell dysfunction. Frontiers in Immunology, 2022 (PubMed: 36177003) [IF=5.7]

Application: IHC    Species: Human    Sample:

Figure 2 Prognostic significance of MRGPI-based groups. (A) Multivariate Cox regression analysis of 13 differentially expressed macrophage-related hub genes. (B) Cox regression analysis of the MRGPI and clinicopathological parameters based on the TCGA cohort. Covariates including age, gender, IDH mutation, ATRX mutation, MGMT promoter methylation status, TERT promoter mutation, and MRGPI were included in the initial univariate Cox regression. Covariates with p-values less than 0.01 were further included in the multivariate Cox model. (C, D) K-M analysis of the survival and tumor progression-free interval differences between MRGPI-based groups based on TCGA, CGGA325, and Gravendeel GBM cohorts. (E, F) Immunohistochemical staining of five genes at the protein level. The tissue was divided into core of the tumor (Tumor) and the margin containing infiltrating tumor cells (Peritumor) in three patients with a pathological diagnosis of GBM. The intensity of staining for the proteins encoded by these genes at the tissue level ranged from negative to positive, with E showing genes with significantly higher IHC scores in the tumor core. The IHC scores of the five genes were shown in F (Scale bar, 100μm). (G) Pan-cancer-based MRGPI prognostic significance. Samples were split into early (PFI < 6 months) and late (PFI > 12 months) relapse groups based on PFI. OV, Ovarian serous cystadenocarcinoma; LUAD, Lung adenocarcinoma; LUSC, Lung squamous cell carcinoma; PRAD, Prostate adenocarcinoma; BLCA, Bladder urothelial carcinoma; TGCT, Testicular germ cell tumors; ESCA, Esophageal carcinoma; PAAD, Pancreatic adenocarcinoma; LIHC, Liver hepatocellular carcinoma; KIRP, Kidney renal papillary cell carcinoma; SARC, Sarcoma; BRCA, Breast invasive carcinoma; MESO, Mesothelioma; COAD, Colon adenocarcinoma; STAD, Stomach adenocarcinoma; SKCM, Skin cutaneous melanoma; CHOL, Cholangiocarcinoma; KIRC, Kidney renal clear cell carcinoma; THCA, Thyroid carcinoma; UCEC, Uterine corpus endometrial carcinoma; CESC, Cervical squamous cell carcinoma and endocervical adenocarcinoma; HNSC, Head and neck squamous cell carcinoma; READ, Rectum adenocarcinoma; LGG, Lower grade glioma; KICH, Kidney chromophobe; UCS, Uterine carcinosarcoma; ACC, Adrenocortical carcinoma; PCPG, Pheochromocytoma and paraganglioma; UVM, Uveal melanoma. ***p < 0.001. ns, non significant.

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