CCR2 Antibody - #DF2711

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产品: CCR2 抗体
货号: DF2711
描述: Rabbit polyclonal antibody to CCR2
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P41597
RRID: AB_2839917

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
CCR2 Antibody detects endogenous levels of total CCR2.
RRID:
AB_2839917
引用格式: Affinity Biosciences Cat# DF2711, RRID:AB_2839917.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C C chemokine receptor type 2; C C CKR 2; C-C chemokine receptor type 2; C-C CKR-2; CC chemokine receptor type 2; CC CKR 2; CC-CKR-2; CCCKR2; CCR 2; CCR-2; CCR1L; CCR2; CCR2_HUMAN; CCR2A; CCR2B; CCR5L; CD192; CD192 antigen; Chemokine C C motif receptor 2; Chemokine CC Motif Receptor 2; CKR 2; CKR2; CKR2A; CKR2B; CMKBR2; MCP 1 R; MCP-1-R; MCP1 RECEPTOR; MCP1R; Monocyte chemoattractant protein 1 receptor; Monocyte Chemotactic Protein 1 Receptor; Monocyte Chemotactic Protein 1 Receptor;

抗原和靶标

免疫原:

A synthesized peptide derived from human CCR2, corresponding to a region within the internal amino acids.

基因/基因ID:
CCR2(729230)
描述:
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

文献引用

1). Bioinspired nanovehicle of furoxans-oxaliplatin improves tumoral distribution for chemo-radiotherapy. JOURNAL OF CONTROLLED RELEASE, 2023 (PubMed: 36470332) [IF=10.5]
2). Revealing potential anti-fibrotic mechanism of Ganxianfang formula based on RNA sequence. Chinese medicine, 2022 (PubMed: 35180857) [IF=5.3]

Application: WB    Species: Rat    Sample: liver tissues

Fig. 5. GXF down-regulated genes related to macrophages. A The expression of genes enriched in chemokine pathway. Gene expression level was measured in FPKM value. B The mRNA expression of CCL2, CCR2, CX3CL1, CX3CR1, CXCL16 and CXCR6 were evaluated by RT-qPCR. C The protein levels of CCL2 and CCR2 were evaluated by western blot. D Serum CCL2 level was evaluated by Elisa. The data are presented as means ± SEM (n = 4). #p 
3). Zhiqiao Gancao Decoction Ameliorates Hyperalgesia in Lumbar Disc Herniation via the CCL2/CCR2 Signaling Pathway. Drug Design, Development and Therapy, 2023 (PubMed: 37533973) [IF=4.7]

Application: IF/ICC    Species: Rat    Sample:

Figure 4 CCL2 induces the expression of CCR2 and ASIC3 in DRGn, which is inhibited by CCR2 inhibitors and ZQGCD containing serum (A) The cell viability of DRGn. After 12 h pretreatment with RS504393 and ZQGCD containing serum (5%, 10%, and 15%), there was no change in cell viability (P>0.05). After adding CCL2 for 24 h, the viability of unpretreated cells decreased significantly (P0.05). (B) Expression of CCR2 and ASIC3 mRNA in DRGn. (C and D) Expression of CCR2 and ASIC3 proteins in DRGn. **P

Application: IHC    Species: Rat    Sample:

Figure 8 The expression of CCL2/CCR2 pathway in the intervertebral disc of LDH rats was increased, but CCR2 inhibitors or ZQGCD inhibited this expression. (A and B) Representative images (magnification: 40x) and semi-quantitative histogram of immunohistochemical staining of CCL2 protein of intervertebral disc. (C and D) Representative images (magnification: 40x) and semi-quantitative histogram of immunohistochemical staining of CCR2 protein of intervertebral disc.

Application: WB    Species: Rat    Sample:

Figure 9 CCL2/CCR2 pathway and ASIC3 were highly in DRG of LDH rats expressed but inhibited by CCR2 inhibitor or ZQGCD. (A) Bar graphs showing the expression levels of CCL2 and CCR2 mRNA in DRG. (B and C) Immunoblot and bar graphs showing the expression levels of CCL2 and CCR2 in DRG.
4). Crisaborole combined with vitamin D demonstrates superior therapeutic efficacy over either monotherapy in mice with allergic contact dermatitis. Scientific reports, 2024 (PubMed: 39209980) [IF=3.8]
5). C-C chemokine receptor type 2-overexpressing exosomes alleviated experimental post-stroke cognitive impairment by enhancing microglia/macrophage M2 polarization. World Journal of Stem Cells, 2020 (PubMed: 32184939) [IF=3.6]

Application: WB    Species: human    Sample: HUC-MSCsCtrlvs HUC-MSCsCCR2

Figure 1 |Human umbilical cord mesenchymal stem cellsCCR2 load the C-C chemokine receptor type 2 receptor into their exosomes. A, B: Flow cytometryanalysis of the C-C chemokine receptor type 2 (CCR2) receptor on human umbilical cord mesenchymal stem cells (HUC-MSCs)Ctrl and HUC-MSCsCCR2, n = 3, cP <0.001; C: CCR2 mRNA expression in HUC-MSCsCtrlvs HUC-MSCsCCR2, n = 3, cP < 0.001; D: Western blotting analysis for the quantification of the CCR2 expressionin HUC-MSCsCtrlvs HUC-MSCsCCR2, n = 3
6). Berberine attenuates non-alcoholic steatohepatitis by regulating chemerin/CMKLR1 signalling pathway and Treg/Th17 ratio. Naunyn-Schmiedeberg's archives of pharmacology, 2021 (PubMed: 32524150) [IF=3.1]

Application: WB    Species: rat    Sample: liver

Fig. 4 |Berberine modulates intrahepatic CCR2+ inflammatory cells. a, bThe protein level of CCR2 was measured by Western blot analysis and normalized by GAPDH expression
7). Anti-inflammatory activity of berberine in non-alcoholic fatty liver disease via the Angptl2 pathway. BMC IMMUNOLOGY, 2020 (PubMed: 32429849) [IF=3.0]

Application: WB    Species: rat    Sample: liver

Fig. 5 |The protein levels of Angptl2, Foxo1, NF-κB, p-NF-κB and CCR2 were measured by Western blot analysis and normalized by GAPDH expression. Data are presented as mean ± SD from eight rats. **P < 0.01,* P < 0.05.n = 8/group
8). Resveratrol reduces the inflammatory response in adipose tissue and improves adipose insulin signaling in high-fat diet-fed mice. PeerJ, 2018 (PubMed: 29967759) [IF=2.3]

Application: WB    Species: mouse    Sample: SAT（subcutaneous adiposet issue）

Figure 7 |Effects of resveratrol treatment on the protein expression of CCR2, IRS-1, pAkt (phosphorylation at Ser 473) and GLUT4 in SAT and VAT. (A) Western blots of CCR2 and insulin signaling molecules in SAT

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