NUAK2 Antibody - #DF2671

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产品: NUAK2 抗体
货号: DF2671
描述: Rabbit polyclonal antibody to NUAK2
应用: WB IHC
文献验证: WB, IHC
反应: Human, Rat
预测: Pig, Horse, Sheep, Rabbit, Dog
分子量: 70 kDa; 70kD(Calculated).
蛋白号: Q9H093
RRID: AB_2839877

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Rat
预测:
Pig(89%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
NUAK2 Antibody detects endogenous levels of total NUAK2.
RRID:
AB_2839877
引用格式: Affinity Biosciences Cat# DF2671, RRID:AB_2839877.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp434J037; DKFZp686F01113; FLJ90349; NUAK family SNF1 like kinase 2; NUAK family SNF1-like kinase 2; Nuak2; NUAK2_HUMAN; Omphalocele kinase 2; SNARK; SNF1/AMP activated protein kinase; SNF1/AMP kinase related kinase; SNF1/AMP kinase-related kinase;

抗原和靶标

免疫原:
Uniprot:

Q9H093(NUAK2_HUMAN) >>Visit HPA database.

基因/基因ID:
NUAK2(81788)
描述:
Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells.;
序列:
MESLVFARRSGPTPSAAELARPLAEGLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLGKGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMSSLNHPHIIAIHEVFENSSKIVIVMEYASRGDLYDYISERQQLSEREARHFFRQIVSAVHYCHQNRVVHRDLKLENILLDANGNIKIADFGLSNLYHQGKFLQTFCGSPLYASPEIVNGKPYTGPEVDSWSLGVLLYILVHGTMPFDGHDHKILVKQISNGAYREPPKPSDACGLIRWLLMVNPTRRATLEDVASHWWVNWGYATRVGEQEAPHEGGHPGSDSARASMADWLRRSSRPLLENGAKVCSFFKQHAPGGGSTTPGLERQHSLKKSRKENDMAQSLHSDTADDTAHRPGKSNLKLPKGILKKKVSASAEGVQEDPPELSPIPASPGQAAPLLPKKGILKKPRQRESGYYSSPEPSESGELLDAGDVFVSGDPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
89
Sheep
89
Zebrafish
78
Chicken
78
Xenopus
67
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1.

翻译修饰:

Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro.

蛋白家族:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

文献引用

1). Upregulation of NUAK2: A novel prognostic marker in breast cancer. HISTOLOGY AND HISTOPATHOLOGY, 2022 (PubMed: 36440828) [IF=2.5]

Application: WB    Species: Human    Sample: breast cancer tissues and cell

Fig. 1. Expression of NUAK2 is elevated in breast cancer tissues and cell lines. A. RT-qPCR was performed to determine the expression of NUAK2 in the breast cancer and normal tissues. B. Western blotting assays were performed to assess the protein levels of NUAK2 in 10 breast cancer tissues with paired adjacent non-tumoral tissues. C. Western blotting analysis of NUAK2 protein expression in breast cancer cell lines and primary cultured human mammary epithelial cell (HEMC).

Application: IHC    Species: Human    Sample: breast cancer tissues and cell

Fig. 2. Immunochemistry analyses of NUAK2 expression in breast cancer tissue samples. Representative images of negative staining of NUAK2 (A), weakly positive staining (+) of NUAK2 (B), positive staining (++) of NUAK2 (C) and strongly positive staining (+++) of NUAK2 (D). x 400.

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