XRCC5 Antibody - #BF0183
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0183, RRID:AB_2833930.
展开/折叠
86 kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 80 kDa subunit; ATP dependent DNA helicase II 86 Kd subunit; ATP dependent DNA helicase II; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box binding factor 85 kDa; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; KARP 1; KARP1; Ku 80; Ku autoantigen 80kDa; Ku80; Ku86; Ku86 autoantigen related protein 1; KUB 2; KUB2; Lupus Ku autoantigen protein p86; NFIV; Nuclear factor IV; Thyroid lupus autoantigen; Thyroid-lupus autoantigen; TLAA; X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining); X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair cross-complementing protein 5; Xray repair complementing defective repair in Chinese hamster cells 5; XRCC 5; XRCC5; XRCC5_HUMAN;
抗原和靶标
Purified recombinant fragment of human XRCC5 expressed in E. Coli.
- P13010 XRCC5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAKKVITMFVQRQVFAENKDEIALVLFGTDGTDNPLSGGDQYQNITVHRHLMLPDFDLLEDIESKIQPGSQQADFLDALIVSMDVIQHETIGKKFEKRHIEIFTDLSSRFSKSQLDIIIHSLKKCDISLQFFLPFSLGKEDGSGDRGDGPFRLGGHGPSFPLKGITEQQKEGLEIVKMVMISLEGEDGLDEIYSFSESLRKLCVFKKIERHSIHWPCRLTIGSNLSIRIAAYKSILQERVKKTWTVVDAKTLKKEDIQKETVYCLNDDDETEVLKEDIIQGFRYGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQVQRRFFMGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVAIVRYAYDKRANPQVGVAFPHIKHNYECLVYVQLPFMEDLRQYMFSSLKNSKKYAPTEAQLNAVDALIDSMSLAKKDEKTDTLEDLFPTTKIPNPRFQRLFQCLLHRALHPREPLPPIQQHIWNMLNPPAEVTTKSQIPLSKIKTLFPLIEAKKKDQVTAQEIFQDNHEDGPTAKKLKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEASNQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI
翻译修饰 - P13010 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K36 | Ubiquitination | Uniprot | |
T39 | Phosphorylation | Uniprot | |
K51 | Sumoylation | Uniprot | |
S102 | Phosphorylation | Uniprot | |
K125 | Ubiquitination | Uniprot | |
R141 | Methylation | Uniprot | |
K144 | Acetylation | Uniprot | |
K144 | Ubiquitination | Uniprot | |
S145 | Phosphorylation | Uniprot | |
K155 | Acetylation | Uniprot | |
K155 | Ubiquitination | Uniprot | |
K156 | Ubiquitination | Uniprot | |
K171 | Ubiquitination | Uniprot | |
S175 | Phosphorylation | Uniprot | |
S191 | Phosphorylation | Uniprot | |
K195 | Acetylation | Uniprot | |
K195 | Sumoylation | Uniprot | |
K195 | Ubiquitination | Uniprot | |
T198 | Phosphorylation | Uniprot | |
K202 | Ubiquitination | Uniprot | |
Y225 | Phosphorylation | Uniprot | |
K233 | Ubiquitination | Uniprot | |
C235 | S-Nitrosylation | Uniprot | |
K238 | Ubiquitination | Uniprot | |
S244 | Phosphorylation | Uniprot | |
C249 | S-Nitrosylation | Uniprot | |
S255 | Phosphorylation | Uniprot | |
S258 | Phosphorylation | Uniprot | |
K265 | Acetylation | Uniprot | |
K265 | Ubiquitination | Uniprot | |
K274 | Ubiquitination | Uniprot | |
T277 | Phosphorylation | Uniprot | |
K282 | Ubiquitination | Uniprot | |
K285 | Sumoylation | Uniprot | |
K285 | Ubiquitination | Uniprot | |
K291 | Ubiquitination | Uniprot | |
Y295 | Phosphorylation | Uniprot | |
C296 | S-Nitrosylation | Uniprot | |
K307 | Sumoylation | Uniprot | |
K307 | Ubiquitination | Uniprot | |
S318 | Phosphorylation | Uniprot | |
S324 | Phosphorylation | Uniprot | |
K325 | Sumoylation | Uniprot | |
K325 | Ubiquitination | Uniprot | |
K332 | Acetylation | Uniprot | |
K332 | Methylation | Uniprot | |
K332 | Sumoylation | Uniprot | |
K332 | Ubiquitination | Uniprot | |
K334 | Methylation | Uniprot | |
K334 | Ubiquitination | Uniprot | |
S335 | Phosphorylation | Uniprot | |
K338 | Acetylation | Uniprot | |
K338 | Sumoylation | Uniprot | |
K338 | Ubiquitination | Uniprot | |
C339 | S-Nitrosylation | Uniprot | |
S341 | Phosphorylation | Uniprot | |
C346 | S-Nitrosylation | Uniprot | |
K347 | Ubiquitination | Uniprot | |
K363 | Acetylation | Uniprot | |
K363 | Ubiquitination | Uniprot | |
S378 | Phosphorylation | Uniprot | |
S379 | Phosphorylation | Uniprot | |
K399 | Acetylation | Uniprot | |
K399 | Ubiquitination | Uniprot | |
Y416 | Phosphorylation | Uniprot | |
S436 | Phosphorylation | Uniprot | |
S437 | Phosphorylation | Uniprot | |
K439 | Ubiquitination | Uniprot | |
K443 | Acetylation | Uniprot | |
K443 | Sumoylation | Uniprot | |
K443 | Ubiquitination | Uniprot | |
K465 | Ubiquitination | Uniprot | |
K466 | Acetylation | Uniprot | |
K466 | Ubiquitination | Uniprot | |
K469 | Ubiquitination | Uniprot | |
T472 | Phosphorylation | Uniprot | |
K481 | Ubiquitination | Uniprot | |
C493 | S-Nitrosylation | Uniprot | |
T523 | Phosphorylation | Uniprot | |
T524 | Phosphorylation | Uniprot | |
K525 | Ubiquitination | Uniprot | |
S531 | Phosphorylation | Uniprot | |
K532 | Acetylation | Uniprot | |
K532 | Sumoylation | Uniprot | |
K532 | Ubiquitination | Uniprot | |
K534 | Acetylation | Uniprot | |
K534 | Sumoylation | Uniprot | |
K534 | Ubiquitination | Uniprot | |
T535 | Phosphorylation | Uniprot | |
K543 | Acetylation | Uniprot | |
K543 | Sumoylation | Uniprot | |
K543 | Ubiquitination | Uniprot | |
K544 | Ubiquitination | Uniprot | |
K545 | Ubiquitination | Uniprot | |
K565 | Acetylation | Uniprot | |
K565 | Sumoylation | Uniprot | |
K565 | Ubiquitination | Uniprot | |
K566 | Acetylation | Uniprot | |
K566 | Ubiquitination | Uniprot | |
K568 | Sumoylation | Uniprot | |
T569 | Phosphorylation | Uniprot | |
S577 | Phosphorylation | P78527 (PRKDC) | Uniprot |
S579 | Phosphorylation | Uniprot | |
S580 | Phosphorylation | P78527 (PRKDC) | Uniprot |
S585 | Phosphorylation | Uniprot | |
T629 | Phosphorylation | Uniprot | |
R640 | Methylation | Uniprot | |
K648 | Acetylation | Uniprot | |
K648 | Ubiquitination | Uniprot | |
K660 | Acetylation | Uniprot | |
K660 | Methylation | Uniprot | |
K660 | Sumoylation | Uniprot | |
K660 | Ubiquitination | Uniprot | |
K665 | Acetylation | Uniprot | |
K665 | Ubiquitination | Uniprot | |
S692 | Phosphorylation | Uniprot | |
S695 | Phosphorylation | Uniprot | |
K702 | Acetylation | Uniprot | |
K702 | Methylation | Uniprot | |
K702 | Ubiquitination | Uniprot | |
K710 | Ubiquitination | Uniprot | |
S712 | Phosphorylation | Uniprot | |
T715 | Phosphorylation | P78527 (PRKDC) | Uniprot |
研究背景
Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
ADP-ribosylated by PARP3.
Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
Sumoylated.
Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites. Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks.
Nucleus. Nucleus>Nucleolus. Chromosome.
Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. The dimer also associates with NAA15, and this complex displays DNA binding activity towards the osteocalcin FGF response element (OCFRE). In addition, XRCC5 binds to the osteoblast-specific transcription factors MSX2 and RUNX2. Interacts with ELF3. May interact with APLF. The XRCC5/XRCC6 dimer associates in a DNA-dependent manner with APEX1. Identified in a complex with DEAF1 and XRCC6. Interacts with NR4A3; the DNA-dependent protein kinase complex DNA-PK phosphorylates and activates NR4A3 and prevents NR4A3 ubiquitinylation and degradation. Interacts with RNF138. Interacts with CYREN isoform 1 (CYREN-1) and isoform 4 (CYREN-2). Interacts (via N-terminus) with HSF1 (via N-terminus); this interaction is direct and prevents XRCC5/XRCC6 heterodimeric binding and non-homologous end joining (NHEJ) repair activities induced by ionizing radiation (IR). Interacts with DHX9; this interaction occurs in a RNA-dependent manner. Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA. Interacts with ERCC6.
(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein HBZ.
The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
Belongs to the ku80 family.
研究领域
· Genetic Information Processing > Replication and repair > Non-homologous end-joining.
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