PI 3 Kinase Class 2A Antibody - #DF2623

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产品: PI 3 Kinase Class 2A 抗体
货号: DF2623
描述: Rabbit polyclonal antibody to PI 3 Kinase Class 2A
应用: WB
反应: Human, Mouse
预测: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 191 kDa; 191kD(Calculated).
蛋白号: O00443
RRID: AB_2839829

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)
克隆:
Polyclonal
特异性:
PI 3 Kinase Class 2A Antibody detects endogenous levels of total PI 3 Kinase Class 2A.
RRID:
AB_2839829
引用格式: Affinity Biosciences Cat# DF2623, RRID:AB_2839829.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C2 containing phosphatidylinositol kinase; C2 domain containing alpha polypeptide; Class 2 alpha polypeptide; CPK; Cpk m; DKFZp686L193; MGC142218; OTTHUMP00000231763; P3C2A_HUMAN; Phosphatidylinositol 3 kinase class 2 alpha; Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha; Phosphoinositide 3 kinase class 2 alpha; Phosphoinositide 3 kinase, class 2, alpha polypeptide; Phosphoinositide 3-Kinase-C2-alpha; PI3-K-C2(ALPHA); PI3-K-C2A; PI3K-C2-alpha; PI3K-C2alpha; PI3KC 2 alpha; PIK3C 2A; Pik3c2a; PtdIns-3-kinase C2 subunit alpha;

抗原和靶标

免疫原:
Uniprot:

O00443(P3C2A_HUMAN) >>Visit HPA database.

基因/基因ID:
PIK3C2A(5286)
表达:
O00443 P3C2A_HUMAN:

Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney. Detected at low levels in islets of Langerhans from type 2 diabetes mellitus individuals.

描述:
Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. May play a role in clathrin-coated endocytic vesicle formation and EGF signaling cascade. May be involved in mitosis and UV-induced damage response. May be a downstream effector in insulin signaling cascade.
序列:
MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQSLNIRTTQLAKAQGHISQKDPNGTSSLPTGSSLLQEVEVQNEEMAAFCRSITKLKTKFPYTNHRTNPGYLLSPVTAQRNICGENASVKVSIDIEGFQLPVTFTCDVSSTVEIIIMQALCWVHDDLNQVDVGSYVLKVCGQEEVLQNNHCLGSHEHIQNCRKWDTEIRLQLLTFSAMCQNLARTAEDDETPVDLNKHLYQIEKPCKEAMTRHPVEELLDSYHNQVELALQIENQHRAVDQVIKAVRKICSALDGVETLAITESVKKLKRAVNLPRSKTADVTSLFGGEDTSRSSTRGSLNPENPVQVSINQLTAAIYDLLRLHANSGRSPTDCAQSSKSVKEAWTTTEQLQFTIFAAHGISSNWVSNYEKYYLICSLSHNGKDLFKPIQSKKVGTYKNFFYLIKWDELIIFPIQISQLPLESVLHLTLFGILNQSSGSSPDSNKQRKGPEALGKVSLPLFDFKRFLTCGTKLLYLWTSSHTNSVPGTVTKKGYVMERIVLQVDFPSPAFDIIYTTPQVDRSIIQQHNLETLENDIKGKLLDILHKDSSLGLSKEDKAFLWEKRYYCFKHPNCLPKILASAPNWKWVNLAKTYSLLHQWPALYPLIALELLDSKFADQEVRSLAVTWIEAISDDELTDLLPQFVQALKYEIYLNSSLVQFLLSRALGNIQIAHNLYWLLKDALHDVQFSTRYEHVLGALLSVGGKRLREELLKQTKLVQLLGGVAEKVRQASGSARQVVLQRSMERVQSFFQKNKCRLPLKPSLVAKELNIKSCSFFSSNAVPLKVTMVNADPMGEEINVMFKVGEDLRQDMLALQMIKIMDKIWLKEGLDLRMVIFKCLSTGRDRGMVELVPASDTLRKIQVEYGVTGSFKDKPLAEWLRKYNPSEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLRSTGHMFHIDFGKFLGHAQMFGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQAYNLIRKQTNLFLNLLSLMIPSGLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIESSLGSIATKFNFFIHNLAQLRFSGLPSNDEPILSFSPKTYSFRQDGRIKEVSVFTYHKKYNPDKHYIYVVRILREGQIEPSFVFRTFDEFQELHNKLSIIFPLWKLPGFPNRMVLGRTHIKDVAAKRKIELNSYLQSLMNASTDVAECDLVCTFFHPLLRDEKAEGIARSADAGSFSPTPGQIGGAVKLSISYRNGTLFIMVMHIKDLVTEDGADPNPYVKTYLLPDNHKTSKRKTKISRKTRNPTFNEMLVYSGYSKETLRQRELQLSVLSAESLRENFFLGGVTLPLKDFNLSKETVKWYQLTAATYL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
91
Xenopus
90
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O00443 作为底物

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
S6 Phosphorylation
S8 Phosphorylation
K11 Ubiquitination
T49 Phosphorylation
S60 Phosphorylation
Y68 Phosphorylation
Y73 Phosphorylation
K94 Acetylation
K94 Ubiquitination
K102 Ubiquitination
S108 Phosphorylation
K112 Ubiquitination
T120 Phosphorylation
S124 Phosphorylation
S126 Phosphorylation
R139 Methylation
R175 Methylation
K229 Ubiquitination
S232 Phosphorylation
T233 Phosphorylation
S234 Phosphorylation
K238 Ubiquitination
S251 Phosphorylation
K252 Ubiquitination
S259 Phosphorylation P06493 (CDK1)
K261 Ubiquitination
K267 Ubiquitination
K278 Ubiquitination
K280 Methylation
K280 Ubiquitination
K293 Ubiquitination
K301 Ubiquitination
S327 Phosphorylation
S329 Phosphorylation
S336 Phosphorylation
S338 Phosphorylation
K348 Ubiquitination
K390 Ubiquitination
K394 Ubiquitination
Y406 Phosphorylation
S409 Phosphorylation
T412 Phosphorylation
K532 Acetylation
K532 Ubiquitination
K539 Ubiquitination
C541 S-Nitrosylation
K542 Ubiquitination
K579 Ubiquitination
S612 Phosphorylation
K613 Ubiquitination
T614 Phosphorylation
S630 Phosphorylation
S665 Phosphorylation
T667 Phosphorylation
S672 Phosphorylation
S673 Phosphorylation
K674 Ubiquitination
Y737 Phosphorylation
S771 Phosphorylation
S772 Phosphorylation
S778 Phosphorylation
K783 Ubiquitination
K872 Acetylation
K872 Ubiquitination
K874 Ubiquitination
K881 Acetylation
K881 Ubiquitination
K889 Acetylation
K889 Ubiquitination
K892 Ubiquitination
K898 Ubiquitination
Y900 Phosphorylation
Y901 Phosphorylation
K920 Ubiquitination
K1062 Ubiquitination
S1084 Phosphorylation
K1088 Ubiquitination
K1096 Ubiquitination
K1158 Ubiquitination
K1162 Ubiquitination
K1209 Ubiquitination
K1217 Ubiquitination
S1281 Phosphorylation
S1412 Phosphorylation
K1414 Ubiquitination
S1551 Phosphorylation
S1553 Phosphorylation
Y1595 Phosphorylation
K1597 Ubiquitination
K1606 Ubiquitination
K1611 Acetylation
Y1632 Phosphorylation
S1633 Phosphorylation
K1672 Ubiquitination

翻译修饰 - O00443 作为激酶

Substrate Site Source
P06730 (EIF4E) S209 Uniprot
P06730 (EIF4E) T210 Uniprot

研究背景

功能:

Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components.

翻译修饰:

Phosphorylated upon insulin stimulation; which may lead to enzyme activation (By similarity). Phosphorylated on Ser-259 during mitosis and upon UV irradiation; which does not change enzymatic activity but leads to proteasomal degradation. Ser-259 phosphorylation may be mediated by CDK1 or JNK, depending on the physiological state of the cell.

细胞定位:

Cell membrane. Golgi apparatus. Cytoplasmic vesicle>Clathrin-coated vesicle. Nucleus. Cytoplasm.
Note: Inserts preferentially into membranes containing PtdIns(4,5)P2 (PubMed:17038310). Associated with RNA-containing structures (PubMed:11606566).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney. Detected at low levels in islets of Langerhans from type 2 diabetes mellitus individuals.

亚基结构:

Part of a complex with ERBB2 and EGFR. Interacts with clathrin trimers. Interacts with SBF2/MTMR13 (By similarity).

蛋白家族:

Belongs to the PI3/PI4-kinase family.

研究领域

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). C/EBPα-Mediated Transcriptional Activation of PIK3C2A Regulates Autophagy, Matrix Metalloproteinase Expression, and Phenotypic of Vascular Smooth Muscle Cells in Aortic Dissection. Journal of Immunology Research (PubMed: 36132983) [IF=4.1]

Application: WB    Species: Rat    Sample: aorta tissues

Figure 4 The impact of C/EBPα in angiotensin II- (Ang II-) treated VSMCs. The relative protein expressions in Ang II-treated VSMCs with presence of C/EBPα overexpression or knockdown were analyzed by Western blot. GAPDH was used as an internal control. Data are presented as means ± SEM. (a, b) Evaluation of the influence of C/EBPα on the expression of α-SMA, SM-MHC, and OPN proteins. (c, d) Evaluation of the influence of C/EBPα on the expression of PIK3C2A protein. (e, f) Evaluation of the influence of C/EBPα on the expression of Beclin-1, p62, LC3II, and LC3I proteins. (g, h) Evaluation of the influence of C/EBPα on the expression of MMP-2 and MMP-9 proteins. (i) Intracellular ROS was measured by flow cytometry using DCFH-DA probe with presence of C/EBPα overexpression or knockdown. Control: control group; model: treated with 1 μM Ang II; vector: treated with 1 μM Ang II in vector transfected VSMCs; pcC/EBPα: treated with 1 μM Ang II in C/EBPα-overexpressed VSMCs; siNC: treated with 1 μM Ang II in siRNA NC-transfected VSMCs; siC/EBPα: treated with 1 μM Ang II in C/EBPα-knockdown VSMCs. ∗P < 0.05 vs. control,

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