| 产品: | ORC5 抗体 |
| 货号: | DF2603 |
| 描述: | Rabbit polyclonal antibody to ORC5 |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 50 kDa; 50kD(Calculated). |
| 蛋白号: | O43913 |
| RRID: | AB_2839809 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2603, RRID:AB_2839809.
展开/折叠
ORC5; ORC5_HUMAN; ORC5L; ORC5P; ORC5T; Origin recognition complex subunit 5; Origin recognition complex subunit 5 like;
抗原和靶标
- O43913 ORC5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPHLENVVLCRESQVSILQSLFGERHHFSFPSIFIYGHTASGKTYVTQTLLKTLELPHVFVNCVECFTLRLLLEQILNKLNHLSSSEDGCSTEITCETFNDFVRLFKQVTTAENLKDQTVYIVLDKAEYLRDMEANLLPGFLRLQELADRNVTVLFLSEIVWEKFRPNTGCFEPFVLYFPDYSIGNLQKILSHDHPPEYSADFYAAYINILLGVFYTVCRDLKELRHLAVLNFPKYCEPVVKGEASERDTRKLWRNIEPHLKKAMQTVYLREISSSQWEKLQKDDTDPGQLKGLSAHTHVELPYYSKFILIAAYLASYNPARTDKRFFLKHHGKIKKTNFLKKHEKTSNHLLGPKPFPLDRLLAILYSIVDSRVAPTANIFSQITSLVTLQLLTLVGHDDQLDGPKYKCTVSLDFIRAIARTVNFDIIKYLYDFL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O43913 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S86 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| Y236 | Phosphorylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K330 | Acetylation | Uniprot |
研究背景
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
Multi-mono-ubiquitinated by OBI1; ubiquitination is important for efficient DNA replication origin site activation. Ubiquitination levels are low in mitotic and early G1-phAse cells and are induced in late G1-/early S-phase, peaking in S-phase and decrease toward the end of the cell cycle.
Nucleus. Chlorosome.
Abundant in spleen, ovary, prostate, testis, and colon mucosa.
Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase.
Belongs to the ORC5 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
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