KIF23 Antibody - #DF2573

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产品: KIF23 抗体
货号: DF2573
描述: Rabbit polyclonal antibody to KIF23
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 89 kDa; 110kD(Calculated).
蛋白号: Q02241
RRID: AB_2839779

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
预测:
Pig(85%), Bovine(%), Horse(%), Sheep(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
KIF23 Antibody detects endogenous levels of total KIF23.
RRID:
AB_2839779
引用格式: Affinity Biosciences Cat# DF2573, RRID:AB_2839779.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CHO 1; CHO1; KIF 23; KIF23; KIF23_HUMAN; Kinesin family member 23; Kinesin like 5; Kinesin like protein 5; Kinesin like protein KIF 23; Kinesin like protein KIF23; Kinesin-like protein 5; Kinesin-like protein KIF23; KNS L5; KNSL 5; KNSL5; Mitotic kinesin like 1; Mitotic kinesin like protein 1; Mitotic kinesin-like protein 1; MKLP 1;

抗原和靶标

免疫原:
Uniprot:

Q02241(KIF23_HUMAN) >>Visit HPA database.

基因/基因ID:
KIF23(9493)
描述:
Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles.
序列:
MKSARAKTPRKPTVKKGSQTNLKDPVGVYCRVRPLGFPDQECCIEVINNTTVQLHTPEGYRLNRNGDYKETQYSFKQVFGTHTTQKELFDVVANPLVNDLIHGKNGLLFTYGVTGSGKTHTMTGSPGEGGLLPRCLDMIFNSIGSFQAKRYVFKSNDRNSMDIQCEVDALLERQKREAMPNPKTSSSKRQVDPEFADMITVQEFCKAEEVDEDSVYGVFVSYIEIYNNYIYDLLEEVPFDPIKPKPPQSKLLREDKNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTHLNRESSRSHSVFNIKLVQAPLDADGDNVLQEKEQITISQLSLVDLAGSERTNRTRAEGNRLREAGNINQSLMTLRTCMDVLRENQMYGTNKMVPYRDSKLTHLFKNYFDGEGKVRMIVCVNPKAEDYEENLQVMRFAEVTQEVEVARPVDKAICGLTPGRRYRNQPRGPVGNEPLVTDVVLQSFPPLPSCEILDINDEQTLPRLIEALEKRHNLRQMMIDEFNKQSNAFKALLQEFDNAVLSKENHMQGKLNEKEKMISGQKLEIERLEKKNKTLEYKIEILEKTTTIYEEDKRNLQQELETQNQKLQRQFSDKRRLEARLQGMVTETTMKWEKECERRVAAKQLEMQNKLWVKDEKLKQLKAIVTEPKTEKPERPSRERDREKVTQRSVSPSPVPLSSNYIAQISNGQQLMSQPQLHRRSNSCSSISVASCISEWEQKIPTYNTPLKVTSIARRRQQEPGQSKTCIVSDRRRGMYWTEGREVVPTFRNEIEIEEDHCGRLLFQPDQNAPPIRLRHRRSRSAGDRWVDHKPASNMQTETVMQPHVPHAITVSVANEKALAKCEKYMLTHQELASDGEIETKLIKGDIYKTRGGGQSVQFTDIETLKQESPNGSRKRRSSTVAPAQPDGAESEWTDVETRCSVAVEMRAGSQLGPGYQHHAQPKRKKP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Xenopus
100
Pig
85
Horse
85
Bovine
85
Sheep
85
Dog
85
Chicken
83
Rabbit
78
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.

翻译修饰:

Ubiquitinated. Deubiquitinated by USP8/UBPY.

细胞定位:

Nucleus. Cytoplasm>Cytoskeleton>Spindle. Midbody>Midbody ring.
Note: Localizes to the interzone of mitotic spindles. Detected at the midbody during later stages of mitotic cytokinesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY. Interacts with ARF6, forming heterodimers and heterotetramers.

蛋白家族:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

研究领域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

文献引用

1). Diagnostic biomarker KIF23 is associated with immune infiltration and immunotherapy response in gastric cancer. Frontiers in Oncology, 2023 (PubMed: 37483517) [IF=3.5]

Application: IHC    Species: Human    Sample:

Figure 2 IHC results about KIF23 protein expression. (A, C, E–H) KIF23 expression in adjacent gastric tissues. (B, D, J–M) KIF23 expression in GC tissues. Magnification: E, G, J, H (×200); F, H, K, M (×400). (I) Rate of KIF23 expression with high and low in GC and adjacent gastric tissues.
2). Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma. American Journal of Translational Research, 2020 (PubMed: 32509165) [IF=1.7]

Application: WB    Species: human    Sample: liver cancer cell

Figure 7. | KIF2C/4A/10/11/14/18B/20A/23 promote cell proliferation in HCC. A. Eight KIFs were evaluated in liver cancer cell lines compared with the normal liver epithelial cell line HL-7702, as analyzed by qRT-PCR. B. Eight distinct KIFs were effectively knocked down by siRNAs.
3). Apoptotic Extracellular Vesicles (ApoEVs) Safeguard Liver Homeostasis and Regeneration via Assembling an ApoEV-Golgi Organelle. bioRxiv, 2021

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