JAD1D Antibody - #DF2548

Figure 1. JARID1D downregulation is associated with PCa metastasis and poor prognosis. (A) JARID1D mRNA levels in PCa tissues were assessed by analyzing the GSE35988. (B and C) Kaplan-Meier analysis of disease-free survival (DFS) and overall survival (OS) curves for patients with PCa with low JARID1D expression vs. those with high JARID1D expression (log-rank test, n = 150). (D) Cell type annotation of 25782 cells using t-distributed stochastic neighbor embedding (t-SNE) and uniform manifold approximation and T-SNE plots showing average expression of gene markers for all cell clusters. (E) T-SNE plot of JARID1D showing normalized expression levels in the sub-clusters. (F) Gene Ontology (GO) enrichment analysis for JARID1D-high-and JARID1D-low-expression groups. (G) Quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of the effect of JARID1D knockdown on the expression of invasiveness-associated genes. (H) Immunohistochemistry (IHC) analysis and quantification of JARID1D expression levels in clinical samples of primary prostate cancer (PCa), local metastatic PCa, and bone metastatic PCa. Scale bars, 50 and 20 μm.

Figure 5. H3K4 modification by JARID1D dynamically regulates AR transcription. (A) Quantitative RT-PCR was used to analyze AR, IL-6, and RANKL expression levels in 22RV1 Sh-JARID1D cells treated with Enz (B) Quantitative RT-PCR was used to analyze AR, IL-6, and RANKL expression levels in DU145 Sh-JARID1D cells treated with R1881. (C) Before protein blotting with mutual antibodies, anti-JARID1D, anti-AR, or control antibodies were used to perform Co-IP on 22RV1 cells grown in serum-containing medium;. (D) RT-PCR analysis of AR and JARID1D mRNA expression levels after transfection with si-RNA in 22RV1 and C42 cells. (E) Western blotting of AR and JARID1D protein expression levels after transfection with siRNA in 22RV1 and C42 cells; (F) mRNA expression level of NSE and CGA. (I, K, and M) Co-localization and expression changes of JARID1D and AR. JARID1D is labeled with red fluorescence, AR is labeled with green fluorescence and the cell nucleus is counterstained with DAPI (blue). (J, L, and N) Histone methylation levels at AR enhancer regions.

Figure 5. H3K4 modification by JARID1D dynamically regulates AR transcription. (A) Quantitative RT-PCR was used to analyze AR, IL-6, and RANKL expression levels in 22RV1 Sh-JARID1D cells treated with Enz (B) Quantitative RT-PCR was used to analyze AR, IL-6, and RANKL expression levels in DU145 Sh-JARID1D cells treated with R1881. (C) Before protein blotting with mutual antibodies, anti-JARID1D, anti-AR, or control antibodies were used to perform Co-IP on 22RV1 cells grown in serum-containing medium;. (D) RT-PCR analysis of AR and JARID1D mRNA expression levels after transfection with si-RNA in 22RV1 and C42 cells. (E) Western blotting of AR and JARID1D protein expression levels after transfection with siRNA in 22RV1 and C42 cells; (F) mRNA expression level of NSE and CGA. (I, K, and M) Co-localization and expression changes of JARID1D and AR. JARID1D is labeled with red fluorescence, AR is labeled with green fluorescence and the cell nucleus is counterstained with DAPI (blue). (J, L, and N) Histone methylation levels at AR enhancer regions.

Fig. 1. Knocking down JARID1D can enhance the invasive ability of PCa cells. (A-B) The expression of JARID1D in PCa cell lines (22RV1, LNCaP, PC3, C4-2 and DU145) was analyzed using quantitative RT-PCR (A) and Western blot (B) and quantitative analysis results. (C-D) The expression of JARID1D after 22RV1 cells transfection into lentivirus was analyzed using Western blot and quantitative analysis results. (C) and quantitative RT-PCR (D). (E) Transwell (left) and its quantification (right) analyzed the changes in the invasion ability of the 22RV1 cells after knockdown of JARID1D when compared with the NC group, *P 

Fig. 5. Curcumin inhibits PCa lung metastasis caused by JARID1D knockdown through EMT in AR-dependent cells in vivo. (A-B) Bioluminescence image (left) and its quantification (right) of (A) whole mouse and (B) mouse lung tissue after treatment with curcumin. (C) H&E staining results of the mouse lung metastasis tumor tissue after curcumin treatment. (D) The body weight of mice treated with curcumin was monitored every two days. (E) The expression level of JARID1D, AR, MMP2, E-cadherin, N-cadherin, Vimentin in the lung metastasis model induced by the injection of 22RV1-JARID1D cells after treatment with curcumin. EMT, epithelial-mesenchymal transition; PCa, prostate cancer; AR, androgen receptor

Figure 1 Comparison of KDM5D expression in tumor cells (A) and benign cells (B) in radical prostatectomy specimens of docetaxel responders and non-responders. (C) Absolute number of Ki-67 index (% of positive cells in correlation to total cell number of evaluated area) in docetaxel responders and non-responders. Data presented as mean ± SEM, statistics: unpaired t-test, *, P