JAD1D Antibody - #DF2548

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产品: JAD1D 抗体
货号: DF2548
描述: Rabbit polyclonal antibody to JAD1D
应用: WB IHC
文献验证: WB, IHC
反应: Human
分子量: 174kDa; 174kD(Calculated).
蛋白号: Q9BY66
RRID: AB_2839754

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
JAD1D Antibody detects endogenous levels of total JAD1D.
RRID:
AB_2839754
引用格式: Affinity Biosciences Cat# DF2548, RRID:AB_2839754.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

H-Y; Histocompatibility Y antigen; Histone demethylase JARID1D; HY; HYA; JAD1D; Jumonji AT rich interactive domain 1D (RBP2 like); Jumonji/ARID domain-containing protein 1D; KDM5D; KDM5D_HUMAN; KIAA0234; Lysine (K) specific demethylase 5D; Lysine demethylase 5D; Lysine-specific demethylase 5D; Protein SmcY; Selected mouse cDNA on Y human homolog of; SMC homolog Y chromosome; Smcy homolog Y linked;

抗原和靶标

免疫原:
Uniprot:

Q9BY66(KDM5D_HUMAN) >>Visit HPA database.

基因/基因ID:
KDM5D(8284)
表达:
Q9BY66 KDM5D_HUMAN:

Expression is highly down-regulated in metastatic prostate tumors.

序列:
MEPGCDEFLPPPECPVFEPSWAEFQDPLGYIAKIRPIAEKSGICKIRPPADWQPPFAVEVDNFRFTPRVQRLNELEAQTRVKLNYLDQIAKFWEIQGSSLKIPNVERKILDLYSLSKIVIEEGGYEAICKDRRWARVAQRLHYPPGKNIGSLLRSHYERIIYPYEMFQSGANHVQCNTHPFDNEVKDKEYKPHSIPLRQSVQPSKFSSYSRRAKRLQPDPEPTEEDIEKHPELKKLQIYGPGPKMMGLGLMAKDKDKTVHKKVTCPPTVTVKDEQSGGGNVSSTLLKQHLSLEPCTKTTMQLRKNHSSAQFIDSYICQVCSRGDEDDKLLFCDGCDDNYHIFCLLPPLPEIPRGIWRCPKCILAECKQPPEAFGFEQATQEYSLQSFGEMADSFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVTVEYGADIHSKEFGSGFPVSNSKQNLSPEEKEYATSGWNLNVMPVLDQSVLCHINADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWYGVPSLAAEHLEEVMKMLTPELFDSQPDLLHQLVTLMNPNTLMSHGVPVVRTNQCAGEFVITFPRAYHSGFNQGYNFAEAVNFCTADWLPAGRQCIEHYRRLRRYCVFSHEELICKMAAFPETLDLNLAVAVHKEMFIMVQEERRLRKALLEKGVTEAEREAFELLPDDERQCIKCKTTCFLSALACYDCPDGLVCLSHINDLCKCSSSRQYLRYRYTLDELPTMLHKLKIRAESFDTWANKVRVALEVEDGRKRSFEELRALESEARERRFPNSELLQRLKNCLSEVEACIAQVLGLVSGQVARMDTPQLTLTELRVLLEQMGSLPCAMHQIGDVKDVLEQVEAYQAEAREALATLPSSPGLLRSLLERGQQLGVEVPEAHQLQQQVEQAQWLDEVKQALAPSAHRGSLVIMQGLLVMGAKIASSPSVDKARAELQELLTIAERWEEKAHFCLEARQKHPPATLEAIIRETENIPVHLPNIQALKEALTKAQAWIADVDEIQNGDHYPCLDDLEGLVAVGRDLPVGLEELRQLELQVLTAHSWREKASKTFLKKNSCYTLLEVLCPCADAGSDSTKRSRWMEKALGLYQCDTELLGLSAQDLRDPGSVIVAFKEGEQKEKEGILQLRRTNSAKPSPLAPSLMASSPTSICVCGQVPAGVGVLQCDLCQDWFHGQCVSVPHLLTSPKPSLTSSPLLAWWEWDTKFLCPLCMRSRRPRLETILALLVALQRLPVRLPEGEALQCLTERAIGWQDRARKALASEDVTALLRQLAELRQQLQAKPRPEEASVYTSATACDPIREGSGNNISKVQGLLENGDSVTSPENMAPGKGSDLELLSSLLPQLTGPVLELPEAIRAPLEELMMEGDLLEVTLDENHSIWQLLQAGQPPDLDRIRTLLELEKFEHQGSRTRSRALERRRRRQKVDQGRNVENLVQQELQSKRARSSGIMSQVGREEEHYQEKADRENMFLTPSTDHSPFLKGNQNSLQHKDSGSSAACPSLMPLLQLSYSDEQQL

研究背景

功能:

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May play a role in spermatogenesis. Involved in transcriptional repression of diverse metastasis-associated genes; in this function seems to cooperate with ZMYND8. Suppresses prostate cancer cell invasion. Regulates androgen receptor (AR) transcriptional activity by demethylating H3K4me3 active transcription marks.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expression is highly down-regulated in metastatic prostate tumors.

亚基结构:

Interacts with PCGF6, MSH5, ZMYND8, AR.

蛋白家族:

The JmjC domain is required for enzymatic activity.

Belongs to the JARID1 histone demethylase family.

文献引用

1). Curcumin blunts epithelial-mesenchymal transition to alleviate invasion and metastasis of prostate cancer through the JARID1D demethylation. Cancer cell international, 2024 (PubMed: 39218854) [IF=5.8]

Application: WB    Species: Mouse    Sample: PCa cells

Fig. 1. Knocking down JARID1D can enhance the invasive ability of PCa cells. (A-B) The expression of JARID1D in PCa cell lines (22RV1, LNCaP, PC3, C4-2 and DU145) was analyzed using quantitative RT-PCR (A) and Western blot (B) and quantitative analysis results. (C-D) The expression of JARID1D after 22RV1 cells transfection into lentivirus was analyzed using Western blot and quantitative analysis results. (C) and quantitative RT-PCR (D). (E) Transwell (left) and its quantification (right) analyzed the changes in the invasion ability of the 22RV1 cells after knockdown of JARID1D when compared with the NC group, *P 

Application: IHC    Species: Mouse    Sample:

Fig. 5. Curcumin inhibits PCa lung metastasis caused by JARID1D knockdown through EMT in AR-dependent cells in vivo. (A-B) Bioluminescence image (left) and its quantification (right) of (A) whole mouse and (B) mouse lung tissue after treatment with curcumin. (C) H&E staining results of the mouse lung metastasis tumor tissue after curcumin treatment. (D) The body weight of mice treated with curcumin was monitored every two days. (E) The expression level of JARID1D, AR, MMP2, E-cadherin, N-cadherin, Vimentin in the lung metastasis model induced by the injection of 22RV1-JARID1D cells after treatment with curcumin. EMT, epithelial-mesenchymal transition; PCa, prostate cancer; AR, androgen receptor
2). KDM5D predicts response to docetaxel chemotherapy in metastatic castration resistant prostate cancer patients. Translational Andrology and Urology, 2021 (PubMed: 34804837) [IF=1.9]

Application: IHC    Species: Human    Sample:

Figure 1 Comparison of KDM5D expression in tumor cells (A) and benign cells (B) in radical prostatectomy specimens of docetaxel responders and non-responders. (C) Absolute number of Ki-67 index (% of positive cells in correlation to total cell number of evaluated area) in docetaxel responders and non-responders. Data presented as mean ± SEM, statistics: unpaired t-test, *, P

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