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| 产品: | HSPA12B 抗体 |
| 货号: | DF2502 |
| 描述: | Rabbit polyclonal antibody to HSPA12B |
| 应用: | WB |
| 文献验证: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 蛋白号: | Q96MM6 |
| RRID: | AB_2839708 |
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse
克隆:
Polyclonal
特异性:
HSPA12B Antibody detects endogenous levels of total HSPA12B.
RRID:
AB_2839708
引用格式: Affinity Biosciences Cat# DF2502, RRID:AB_2839708.
引用格式: Affinity Biosciences Cat# DF2502, RRID:AB_2839708.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
Heat shock 70 kDa protein 12B; HS12B_HUMAN; Hspa12b;
抗原和靶标
免疫原:
A synthesized peptide derived from human HSPA12B, corresponding to a region within N-terminal amino acids.
基因/基因ID:
文献引用
1). Landscape of tissue-specific RNA Editome provides insight into co-regulated and altered gene expression in pigs (Sus-scrofa). RNA Biology, 2021
(PubMed: 34314293)
[IF=3.6]
Application: WB Species: Human Sample: MDS cells
Figure 5. The role of HSPA12B in MDS cell proliferation. (A-B) The mRNA expression level of HSPA12B in MDS cells after si-HSPA12B and HSPA12B-OE transfection. (C)
The protein expression level of HSPA12B was accessed by western blot using GAPDH protein and PCNA (proliferation marker) as an internal control after si-HSPA12B
transfection. Data collected from three independently replicated wells. (D) The HSPA12B protein expression level accessed by western blot using GAPDH protein as
an internal control after HSPA12B-OE transfection. Data collected from three independently replicated wells. (E-F) The CCK-8 assay displayed absorbance at 450 nm in
MDS cells after si-HSPA12B and HSPA12B-OE transfection. Data were collected and calculated as mean ± SD from three independently replicated wells. (G-H) The EdU
positive cells in red after si-HSPA12B and HSPA12B-OE transfection. Nuclei were stained with DAPI (blue). Data were collected from three independently replicated
wells. Scale bar, 100 µm. Significance in (A-B) and (E-F): **P < 0.01.
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