(1)
(5)
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
GRIP1 Antibody detects endogenous levels of total GRIP1.
RRID:
AB_2839706
引用格式: Affinity Biosciences Cat# DF2500, RRID:AB_2839706.
引用格式: Affinity Biosciences Cat# DF2500, RRID:AB_2839706.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
as GRIP; Glutamate receptor-interacting protein 1; GRIP-1; Grip1; GRIP1_HUMAN;
抗原和靶标
免疫原:
A synthesized peptide derived from human GRIP1, corresponding to a region within the internal amino acids.
基因/基因ID:
描述:
May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
文献引用
1). Kaixin-San improves Aβ-induced synaptic plasticity inhibition by affecting the expression of regulation proteins associated with postsynaptic AMPAR expression. Frontiers in pharmacology, 2023
(PubMed: 36865910)
[IF=5.6]
Application: WB Species: Mouse Sample:
FIGURE 4. Effect of KXS on the levels of AMPAR subunits related to the protein expression. The statistical analysis of ABP (A), GRIP1 (B), NSF (C), pGluR1–Ser845 (D), pGluR1–Ser845/GluR1 (E), PKA (α+β) (F), pGluR2–Ser880 (G), pGluR2–Ser880/GluR2 (H), and PKC δ (I) expression was determined using Western blotting. The pictures below the graph indicate the protein exposure; n = 6 per group. Each column with a bar represents the mean ± SD. *p < 0.05 versus the control group; #p < 0.05 versus the Aβ group. During the experiment, GluR1 and pGluR1–Ser845, as well as GluR2 and pGluR2–Ser880, were run simultaneously on the same electrophoretic gel; hence, the same loading control is used in Figures 3D and 4D and in Figures 3E and 4G. The molecular weights of PKA (α + β) and PKC δ are 40 kD and 77 kD, respectively; therefore, they may be separated on one gel and transferred to the same PVDF membrane. The same loading control is used in Figures 4F,I.
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