FGFP1 Antibody - #DF2498

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产品: FGFP1 抗体
货号: DF2498
描述: Rabbit polyclonal antibody to FGFP1
应用: WB IHC
文献验证: WB
反应: Human
预测: Bovine
分子量: 26kDa; 26kD(Calculated).
蛋白号: Q14512
RRID: AB_2839704

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Bovine(86%)
克隆:
Polyclonal
特异性:
FGFP1 Antibody detects endogenous levels of total FGFP1.
RRID:
AB_2839704
引用格式: Affinity Biosciences Cat# DF2498, RRID:AB_2839704.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

17 kDa HBGF-binding protein; 17 kDa heparin binding growth factor binding protein; 17 kDa heparin-binding growth factor-binding protein; FGF binding protein 1; FGF BP; FGF BP1; FGF-binding protein 1; FGF-BP; FGF-BP1; FGFBP; FGFBP-1; Fgfbp1; FGFP1_HUMAN; Fibroblast growth factor binding protein 1 precursor; Fibroblast growth factor-binding protein 1; HBp17;

抗原和靶标

免疫原:
Uniprot:

Q14512(FGFP1_HUMAN) >>Visit HPA database.

基因/基因ID:
FGFBP1(9982)
表达:
Q14512 FGFP1_HUMAN:

Expressed in the suprabasal region of the epidermis, in hair follicles, the basement membrane at the dermo-epidermal junction (occasionally extending into the basement membrane of dermal blood vessels), wounded skin and several invasive squamous cell carcinomas (at protein level). Expressed in normal and wounded skin and various squamous cell carcinomas.

序列:
MKICSLTLLSFLLLAAQVLLVEGKKKVKNGLHSKVVSEQKDTLGNTQIKQKSRPGNKGKFVTKDQANCRWAATEQEEGISLKVECTQLDHEFSCVFAGNPTSCLKLKDERVYWKQVARNLRSQKDICRYSKTAVKTRVCRKDFPESSLKLVSSTLFGNTKPRKEKTEMSPREHIKGKETTPSSLAVTQTMATKAPECVEDPDMANQRKTALEFCGETWSSLCTFFLSIVQDTSC

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
86
Pig
75
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Acts as a carrier protein that release fibroblast-binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth.

细胞定位:

Secreted>Extracellular space. Cell membrane>Peripheral membrane protein.
Note: Extracellular and plasma membrane-associated. Colocalizes with HSPG2 in the pericellular environment of squamous cell carcinomas.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the suprabasal region of the epidermis, in hair follicles, the basement membrane at the dermo-epidermal junction (occasionally extending into the basement membrane of dermal blood vessels), wounded skin and several invasive squamous cell carcinomas (at protein level). Expressed in normal and wounded skin and various squamous cell carcinomas.

亚基结构:

Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGF1, FGF2, FGF7, FGF10, FGF22 and HSPG2.

蛋白家族:

Belongs to the fibroblast growth factor-binding protein family.

文献引用

1). FGFBP1-mediated crosstalk between fibroblasts and pancreatic cancer cells via FGF22/FGFR2 promotes invasion and metastasis of pancreatic cancer. ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2021 (PubMed: 34117747) [IF=3.3]

Application: WB    Species: Human    Sample: PANC-1 and Mia PaCa-2 cells

Figure 1. FGFBP1 silencing in PCCs decreased the expression and secretion of FGF22 in human CAFs cocultured with PCCs (A) Efficiency of FGFBP1 knockdown in PANC-1 and Mia PaCa-2 cells evaluated by qRT-PCR. (B) Efficiency of FGFBP1 knockdown in PANC-1 and Mia PaCa-2 cells evaluated by western blot analysis. (C) Heat map of differentially expressed genes in mouse fibroblasts cocultured with FGFBP1-shRNA-transfected PANC-1 cells compared with mouse fibroblasts cocultured with scramble-transfected PANC-1 cells. (D) mRNA level of FGF22 in human CAFs cocultured with FGFBP1-shRNA-transfected PANC-1 or Mia PaCa-2 cells (CAF-P or CAF-M) compared with that in CAFs cocultured with scramble-transfected PANC-1 or Mia PaCa-2 cells. (E) Protein level of FGF22 in CAF-P or CAF-M compared with that in CAFs cocultured with scramble-transfected PANC-1 or Mia PaCa-2 cells are shown. (F) Secretion of FGF22 in culture medium of CAF-P or CAF-M compared with that in culture medium of CAFs cocultured with scramble-transfected PANC-1 or Mia PaCa-2 cells.

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