CPEB1 Antibody - #DF2462

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产品: CPEB1 抗体
货号: DF2462
描述: Rabbit polyclonal antibody to CPEB1
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q9BZB8
RRID: AB_2839668

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
CPEB1 Antibody detects endogenous levels of total CPEB1.
RRID:
AB_2839668
引用格式: Affinity Biosciences Cat# DF2462, RRID:AB_2839668.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CEBP; CPE binding protein 1; CPE BP1; CPE-binding protein 1; CPE-BP1; CPEB 1; CPEB; CPEB-1; CPEB1; CPEB1 protein; CPEB1_HUMAN; Cytoplasmic polyadenylation binding protein 1; Cytoplasmic polyadenylation element binding protein; Cytoplasmic polyadenylation element binding protein 1; Cytoplasmic polyadenylation element-binding protein 1; FLJ13203; h CEBP; h-CEBP; hCPEB 1; hCPEB-1; MGC34136; MGC60106; mKIAA0940; MKIAA0940 protein;

抗原和靶标

免疫原:

A synthesized peptide derived from human CPEB1, corresponding to a region within the internal amino acids.

基因/基因ID:
CPEB1(64506)
描述:
Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

文献引用

1). MiR-301 regulates the SIRT1/SOX2 pathway via CPEB1 in the breast cancer progression. Molecular Therapy-Oncolytics, 2021 (PubMed: 34377766) [IF=5.3]

Application: WB    Species: Human    Sample: breast cancer cells

Figure 4 Targeted regulation of CPEB1 expression by miR-301. A: Detection results of fluorescence activity of miR-301 and CPEB1; B: RIP experiment to detect the combination of miR-301, CPEB1 and Ago2; C: Detection of CPEB1 expression in cancer and para-cancerous tissues by RT-qPCR; D: Correlation Analysis of miR-301 and CPEB1; E: The expression of CPEB1 in each group of cells was tested by RT-qPCR; F: Western blot was used to detect the protein expression of CPEB1 in each group of cells. The continuous variables were expressed by mean ± SD (standard deviation); * P < 0.05 compared with normal tissues or normal cells; paired sample t-test, independent sample t-test or one-way ANOVA were used to compare the differences of continuous variables among different groups; Pearson correlation is used to analyze the correlation between CPEB1 and miR-301. We repeated the experiment three times to get accurate and stable results.
2). CPEB1 induces autophagy and promotes apoptosis in ovarian granulosa cells of polycystic ovary syndrome. Molecular reproduction and development, 2024 (PubMed: 38616716) [IF=2.7]

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