MYOCD Antibody - #DF2434

FIGURE 3 MBNL1 increases Myocardin expression by binding to UGCU at the 3'-UTR of Myocardin mRNA. A, The 3'-UTR region of Myocardin contains the binding site of MBNL1. B, The binding of MBNL1 to Myocardin mRNA was validated using RIP. (n = 3, *, P < .05). C-F, The changes in Myocardin and MBNL1 in cardiomyocytes with overexpressed or silenced MBNL1 were measured using realtime PCR and Western blotting. (n = 3, *, P < .05, **, P < .01). G and H, Myocardin mRNA residues after 0, 20, 40 and 60 min in cardiomyocytes treated with Actinomycin D (ACD, 5 μg/mL) and with overexpressed or silenced MBNL1were measured using realtime PCR. I, The 3'-UTR region of Myocardin mRNA was transcribed in vitro and labelled with biotin. The combination of MBNL1 protein and 3'-UTR of Myocardin was detected using RNA pulldown assay. J, The RNA probes corresponding to nine prediction sites (probe # 10-18) and their corresponding antisense probes (probe # 1-9) were synthesized and biotin-labelled. The combination site of MBNL1 protein in the 3'-UTR of Myocardin was confirmed using RNA pull-down assay

Fig. 2. |Mhrt regulates myocardin expression through KLF4 in primary cardiomyocytes.(D, E and F) Following knock down of endogenous KLF4 in primary cardiomyocytes, the effect of Mhrt on myocardin mRNA and protein levels was examined (n = 3, *,P < .05, **, P < .01, #, P > .05).