RB6I2 Antibody - #DF2426

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产品: RB6I2 抗体
货号: DF2426
描述: Rabbit polyclonal antibody to RB6I2
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 128kDa; 128kD(Calculated).
蛋白号: Q8IUD2
RRID: AB_2839632

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%)
克隆:
Polyclonal
特异性:
RB6I2 Antibody detects endogenous levels of total RB6I2.
RRID:
AB_2839632
引用格式: Affinity Biosciences Cat# DF2426, RRID:AB_2839632.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cast 2; CAST2; CAZ associated structural protein 2; Elks; ELKS/RAB6 interacting/CAST family member 1; ELKS/RAB6-interacting/CAST family member 1; ERC 1; ERC protein 1; ERC-1; Erc1; ERC1 protein; KIAA1081; MGC12974; RAB6 interacting protein 2; RAB6-interacting protein 2; RAB6IP2; RB6I2_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q8IUD2(RB6I2_HUMAN) >>Visit HPA database.

基因/基因ID:
ERC1(23085)
表达:
Q8IUD2 RB6I2_HUMAN:

Widely expressed. Isoform 2 and isoform 4 are abundantly expressed in brain. Isoform 1 and isoform 3 are predominantly expressed in testis and thyroid, and isoform 1 predominates in other tissues tested.

序列:
MYGSARSVGKVEPSSQSPGRSPRLPRSPRLGHRRTNSTGGSSGSSVGGGSGKTLSMENIQSLNAAYATSGPMYLSDHENVGSETPKSTMTLGRSGGRLPYGVRMTAMGSSPNIASSGVASDTIAFGEHHLPPVSMASTVPHSLRQARDNTIMDLQTQLKEVLRENDLLRKDVEVKESKLSSSMNSIKTFWSPELKKERALRKDEASKITIWKEQYRVVQEENQHMQMTIQALQDELRIQRDLNQLFQQDSSSRTGEPCVAELTEENFQRLHAEHERQAKELFLLRKTLEEMELRIETQKQTLNARDESIKKLLEMLQSKGLSAKATEEDHERTRRLAEAEMHVHHLESLLEQKEKENSMLREEMHRRFENAPDSAKTKALQTVIEMKDSKISSMERGLRDLEEEIQMLKSNGALSTEEREEEMKQMEVYRSHSKFMKNKVEQLKEELSSKEAQWEELKKKAAGLQAEIGQVKQELSRKDTELLALQTKLETLTNQFSDSKQHIEVLKESLTAKEQRAAILQTEVDALRLRLEEKETMLNKKTKQIQDMAEEKGTQAGEIHDLKDMLDVKERKVNVLQKKIENLQEQLRDKEKQMSSLKERVKSLQADTTNTDTALTTLEEALAEKERTIERLKEQRDRDEREKQEEIDNYKKDLKDLKEKVSLLQGDLSEKEASLLDLKEHASSLASSGLKKDSRLKTLEIALEQKKEECLKMESQLKKAHEAALEARASPEMSDRIQHLEREITRYKDESSKAQAEVDRLLEILKEVENEKNDKDKKIAELERQVKDQNKKVANLKHKEQVEKKKSAQMLEEARRREDNLNDSSQQLQDSLRKKDDRIEELEEALRESVQITAEREMVLAQEESARTNAEKQVEELLMAMEKVKQELESMKAKLSSTQQSLAEKETHLTNLRAERRKHLEEVLEMKQEALLAAISEKDANIALLELSSSKKKTQEEVAALKREKDRLVQQLKQQTQNRMKLMADNYEDDHFKSSHSNQTNHKPSPDQIIQPLLELDQNRSKLKLYIGHLTTLCHDRDPLILRGLTPPASYNLDDDQAAWENELQKMTRGQLQDELEKGERDNAELQEFANAILQQIADHCPDILEQVVNALEESS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
90
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8IUD2 作为底物

Site PTM Type Enzyme
Y2 Phosphorylation
S4 Phosphorylation
R6 Methylation
S7 Phosphorylation
K10 Acetylation
S14 Phosphorylation
S15 Phosphorylation
S17 Phosphorylation
R20 Methylation
S21 Phosphorylation
S27 Phosphorylation
T35 Phosphorylation
S37 Phosphorylation
T38 Phosphorylation
S41 Phosphorylation
T53 Phosphorylation
S55 Phosphorylation
Y73 Phosphorylation
S75 Phosphorylation
R93 Methylation
S94 Phosphorylation
S181 Phosphorylation
S191 Phosphorylation
S250 Phosphorylation
C258 S-Nitrosylation
K299 Ubiquitination
S374 Phosphorylation
K488 Ubiquitination
K552 Ubiquitination
K590 Acetylation
K602 Ubiquitination
S603 Phosphorylation
K625 Ubiquitination
K652 Acetylation
K655 Acetylation
K658 Acetylation
K671 Ubiquitination
S674 Phosphorylation
K679 Ubiquitination
S688 Phosphorylation
K691 Ubiquitination
K719 Ubiquitination
S730 Phosphorylation
R736 Methylation
K766 Ubiquitination
K799 Acetylation
K805 Acetylation
S807 Phosphorylation
S824 Phosphorylation
K835 Ubiquitination
K894 Ubiquitination
S901 Phosphorylation
K905 Ubiquitination
K973 Ubiquitination
Y987 Phosphorylation
K1003 Ubiquitination
S1005 Phosphorylation
T1046 Phosphorylation
S1050 Phosphorylation
Y1051 Phosphorylation
K1066 Ubiquitination

研究背景

功能:

Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport.

细胞定位:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm. Membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein. Cell junction>Synapse>Presynaptic cell membrane.
Note: Recruited on Golgi membranes by RAB6A in a GTP-dependent manner (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Isoform 2 and isoform 4 are abundantly expressed in brain. Isoform 1 and isoform 3 are predominantly expressed in testis and thyroid, and isoform 1 predominates in other tissues tested.

亚基结构:

Part of a complex with CHUK, IKBKB and IKBKG. Interacts with CHUK, IKBKB and IKBKG. The interaction with IKBKG is independent of CHUK and IKBKB. Interacts with NFKBIA. Isoform 4 interacts with PPFIA1, and through its C-terminus with the PDZ domains of RIMS1 and RIMS2. Interacts with ERC2/CAST1. Interacts with the GTB-bound forms of RAB6A isoform 1 and isoform 2 and with RAB6B. The interaction was strongest with RAB6B, followed by RAB6A isoform 2 and weakest with RAB6A isoform 1 (By similarity). Interacts with SDCCAG8.

研究领域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

文献引用

1). ZnO NPs induce miR-342-5p mediated ferroptosis of spermatocytes through the NF-κB pathway in mice. JOURNAL OF NANOBIOTECHNOLOGY, 2024 [IF=10.2]

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