Galectin 1 Antibody - #DF2383

Fig. 1. Galectin-1 plays a critical role in GCPM. (A) Representative images of H&E staining and galectin-1 IHC in GC tissues from patients with or without PM. (B) IRS scores of galectin-1 expression in GC tissues from patients with or without PM(n = 132). (C) Data from the GEPIA database showing the expression levels of LGALS1 in GC tissues compared with normal tissues. (D) The TIMER database illustrates LGALS1 expression in tumour and adjacent normal tissues. GEPIA data further indicate that LGALS1 expression correlates with the detailed clinical stage (E) and gross tumour stage (F) of GC. The Kaplan–Meier survival analysis demonstrates the associations between LGALS1 expression and the first-progression survival (G), postprogression survival (H), and overall survival (I) of GC patients. (J) A representative image showing the GCPM model using HGC-27 cells with different levels of galectin-1 expression. (K) Statistical analysis of the number of peritoneal metastatic nodules across the experimental groups. (L) H&E staining confirmed that the peritoneal nodules were adenocarcinoma tissue(400 × magnification). WT: Wild Type; NS: not statistically significant

Fig. 2. Galectin-1 increases the proliferation, invasion, and migration of GC cells. Representative images of PI/calcein staining in HGC-27 (A) and SGC-7901 (B) cell lines with varying levels of galectin-1 expression(200 × magnification). (C) Quantitative analysis of live cell proportions in all experimental groups by PI/calcein-AM staining. Representative images of EdU-stained HGC-27 (D) and SGC-7901 (E) cells with different galectin-1 expression levels(200 × magnification). (F) Quantification of EdU-positive cells in the various groups.(G)Representative images illustrating the migratory and invasive capacities of GC cells with altered galectin-1 expression(200 × magnification). (H) Statistical analyses of the number of migrated and invaded cells, respectively. (I)Representative images from colony formation assays performed in HGC-27 and SGC-7901 cells. (J) Quantitative results of colony-forming units for HGC-27 and SGC-7901 cells, respectively. WT: Wild Type; NS: not statistically significant

Figure 4 Immunofluorescence staining of CK19, Gal-1, Gal-3, and Ki-67 on PTC- and NTG-derived organoids and the matched tumor tissues. Nuclei were counterstained with DAPI (blue). Passage numbers of these organoid lines were: PTC-3_O, P3; PTC-10_O, P5; NTG-1_O, P4. CK19, Cytokeratin 19; Gal-1, Galectin-1; Gal-3, Galectin-3; NTG, nodular thyroid goiter; T, tumor; O, organoid. Scale bar, 100 µm.