GADD45G Antibody - #DF2376

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产品: GADD45G 抗体
货号: DF2376
描述: Rabbit polyclonal antibody to GADD45G
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 17 kDa; 17kD(Calculated).
蛋白号: O95257
RRID: AB_2839584

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Xenopus(%)
克隆:
Polyclonal
特异性:
GADD45G Antibody detects endogenous levels of total GADD45G.
RRID:
AB_2839584
引用格式: Affinity Biosciences Cat# DF2376, RRID:AB_2839584.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CR6; Cytokine responsive protein CR6; Cytokine-responsive protein CR6; DDIT-2; DDIT2; DNA damage-inducible transcript 2 protein; GA45G_HUMAN; Gadd45g; Growth arrest and DNA damage inducible gamma; Growth arrest and DNA damage-inducible protein GADD45 gamma; GRP17;

抗原和靶标

免疫原:
Uniprot:

O95257(GA45G_HUMAN) >>Visit HPA database.

基因/基因ID:
GADD45G(10912)
描述:
Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.
序列:
MTLEEVRGQDTVPESTARMQGAGKALHELLLSAQRQGCLTAGVYESAKVLNVDPDNVTFCVLAAGEEDEGDIALQIHFTLIQAFCCENDIDIVRVGDVQRLAAIVGAGEEAGAPGDLHCILISNPNEDAWKDPALEKLSLFCEESRSVNDWVPSITLPE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.

亚基结构:

Undergoes concentration-dependent homodimerization, which is required for growth inhibititory activity and enhances interaction with PCNA. Interacts with GADD45GIP1. Interacts with PCNA.

蛋白家族:

Two central helices mediate homodimerization through parallel packing.

Belongs to the GADD45 family.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

文献引用

1). Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1. Toxins, 2022 (PubMed: 35737034) [IF=4.2]

Application: IF/ICC    Species: pig    Sample: 3D4/2 cells

Figure 5 Cell immunofluorescence assay of the expression of CXCL8 and GADD45G proteins. (A) The fluorescence intensity and positive percentages of CXCL8. Nuclear staining (blue) and CXCL8-positive cells (green). (B) The fluorescence intensity and positive percentages of GADD45G. Nuclear staining (blue) and GADD45G-positive cells (red). ** Indicates extremely significant differences (p < 0.01). All experiments were repeated 3 times.
2). Gallic acid alleviates Alzheimer's disease by inhibiting p38/MAPK signaling pathway. Journal of Functional Foods, 2025 [IF=3.8]

Application: WB    Species: Mouse    Sample: brain tissue

Fig. 3. Network pharmacological analysis and the verification of differential expressed genes in the KEGG pathway of transcription analysis. (A) Gadd45b and Gadd45g expression level in GSE29378 database. (B) Venn diagram. (C) GO enrichment analysis. (D) KEGG enrichment analysis. (E) Gene expression in apoptosis, autophagy, p53, and MAPK signaling pathways. (F) The mRNA expression levels of genes in the above four pathways. (G) Protein expression of Gadd45b, Gadd45g, p-p38, p38, and p-p38/p38. Data was presented as mean ± SD, n = 3. #p < 0.05 and ##p < 0.01 compared with the Ctrl group. ⁎p < 0.05 and ⁎⁎p < 0.01 compared with the Mod group.

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