HIF1A Antibody - #BF0593

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产品: HIF1A 抗体
货号: BF0593
描述: Mouse monoclonal antibody to HIF1A
应用: WB IHC IF/ICC ELISA
文献验证: WB
反应: Human, Mouse, Monkey
蛋白号: Q16665
RRID: AB_2833903

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 50ul RMB¥ 1800 现货
 100ul RMB¥ 2800 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse
应用:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Monkey
克隆:
Monoclonal [AFB1879]
特异性:
HIF1A antibody detects endogenous levels of total HIF1A.
RRID:
AB_2833903
引用格式: Affinity Biosciences Cat# BF0593, RRID:AB_2833903.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8;

抗原和靶标

免疫原:

Purified recombinant fragment of human HIF1A expressed in E. Coli.

基因/基因ID:
HIF1A(3091)
描述:
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. (provided by RefSeq) Tissue specificity: Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). Poly-3-hydroxybutyrate-co-3-hydroxyvalerate(PHBV)-Polyethylene glycol 20k(PEG20k) as a promising delivery system for PT2399 in the treatment of disc degeneration. Journal of biological engineering, 2024 (PubMed: 38254196) [IF=5.7]
2). Ditan Decoction ameliorates vascular dementia-induced cognitive dysfunction through anti-ferroptosis via the HIF1α pathway: Integrating network pharmacology and experimental validation. Journal of ethnopharmacology, 2025 (PubMed: 39978449) [IF=4.8]
3). Alleviated NCOA4-mediated ferritinophagy protected RA FLSs from ferroptosis in lipopolysaccharide-induced inflammation under hypoxia. Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2024 (PubMed: 38189810) [IF=4.8]
4). Lack of MOF decreases susceptibility to hypoxia and promotes multidrug resistance in hepatocellular carcinoma via HIF-1α. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34540836) [IF=4.6]

Application: WB    Species: Human    Sample: HCC tissues

FIGURE 1 MOF expression is negatively correlated with HIF-1α expression in HCC tissues and in response to hypoxia. (A) Representative western blots of MOF and HIF-1α in HCC tissues (T) and their paired peritumoral tissues (P). (B) IHC staining images of representative HCC tissues with antibodies against human MOF and HIF-1α, and (C) statistical relationship between MOF and HIF-1α scores in HCC. Chi-square values: 0–3, 10.49, df = 3; 1–3, 8.574, df = 3; 2–3, 4.875, df = 3. Scale bar: 100 μm (left in #1 and #2), 50 μm (right in #1 and #2). (D,E) Western blots of MOF and HIF-1α in Huh-7 (upper panel) and Hep3B (lower panel) cells upon exposure to increasing concentrations of CoCl2-mimicked hypoxia for 24 h (D), or to 100 μM CoCl2 over a time course (E). ∗P < 0.05; n.s., not significant.
5). HIF‐1α interacts with Kindlin‐2 and influences breast cancer elasticity: A study based on shear wave elastography imaging. Cancer Medicine, 2020 (PubMed: 32436609) [IF=2.9]

Application: WB    Species: human    Sample: breast cell

FIGURE 3| HIF-1α interacts with Kindlin-2 and influences collagen biogenesis by targeting P4HA1 and FAK. (A-C) HIF-1α was overexpressed with HIF-1α plasmid or knocked down with HIF-1α siRNA (A), HIF-1α mRNA expression level was analyzed by real-time PCR (B), cell lysates were analyzed by western blot with the indicated antibodies. The expression levels of Kindlin-2, p-FAK, and P4HA1 were consistent with HIF-1α expression (C).
6). Xiaoyu Xiezhuo Drink Protects against Ischemia-Reperfusion Acute Kidney Injury in Aged Mice through Inhibiting the TGF-β1/Smad3 and HIF1 Signaling Pathways. BioMed Research International, 2021 (PubMed: 34545331) [IF=2.6]

Application: WB    Species: Mice    Sample: kidney tissues

Figure 10 Effect of XXD on the TGF-β1/Smad3 and HIF1 signaling pathways of kidney tissues in AKI mice. (a) Representative western blots for MMP-9, α-SMA, ICAM-1, TGF-β1, Smad7, HIF1A, and GAPDH. Quantitative analysis of (b) MMP-9, α-SMA, ICAM-1, TGF-β1, Smad7, and HIF1A and normalized with GAPDH. (c) Representative protein bands of p-Smad3, Smad3, and β-actin in XXD-treated AKI mice. (d) Quantitative analysis of p-Smad3 and Smad3 and normalized with β-actin. All data are represented as the mean ± SD (n = 3). ▲P < 0.05, ▲▲P < 0.01 vs. the model group.

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