产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2334, RRID:AB_2839558.
展开/折叠
Dlk; Dual leucine zipper bearing kinase; Dual leucine zipper kinase DLK; Leucine-zipper protein kinase; M3K12_HUMAN; Map3k12; MAPK-upstream kinase; MEKK12; Mitogen-activated protein kinase kinase kinase 12; Mixed lineage kinase; MUK; Protein kinase MUK; ZPK; ZPKP1;
抗原和靶标
- Q12852 M3K12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTHVLQLHEQDAGGPGGAAGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKPHPSRGLLHGNTMEKLIKKRNVPQKLSPHSKRPDILKTESLLPKLDAALSGVGLPGCPKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRTAVPPHEPGGPGSPGGLGGGPSAWEACPPALRGLHHDLLLRKMSSSSPDLLSAALGSRGRGATGGAGDPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPPPVGPGEGVGLLGTGREGTSGRGGSRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELTSSQRWPQSLNMRQSLSTFSSENPSDGEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPPSEVIPGPEPSSLPIPHQELLRERGPPNSEDSDCDSTELDNSNSVDALRPPASLPP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q12852 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T9 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| S500 | Phosphorylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| S555 | Phosphorylation | Uniprot | |
| S609 | Phosphorylation | Uniprot | |
| S615 | Phosphorylation | Uniprot | |
| S634 | Phosphorylation | Uniprot | |
| T642 | Phosphorylation | Uniprot | |
| S645 | Phosphorylation | Uniprot | |
| S695 | Phosphorylation | Uniprot | |
| T699 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot |
研究背景
Part of a non-canonical MAPK signaling pathway. Activated by APOE, enhances the AP-1-mediated transcription of APP, via a MAP kinase signal transduction pathway composed of MAP2K7 and MAPK1/ERK2 and MAPK3/ERK1. May be an activator of the JNK/SAPK pathway.
Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated (By similarity).
The activity of MAP3K12 can be regulated through its proteasomal degradation. APOE, through a receptor-mediated mechanism, activates MAP3K12 by preventing its proteasomal degradation.
Cytoplasm. Cell membrane.
Note: Behaves essentially as an integral membrane protein.
Highly expressed in brain and kidney.
Homodimer (By similarity). Interacts with MBIP.
Interacts with MBIP through the leucine-zipper motif.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
限制条款
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