CENPN Antibody - #DF2315

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产品: CENPN 抗体
货号: DF2315
描述: Rabbit polyclonal antibody to CENPN
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 40 kDa; 40kD(Calculated).
蛋白号: Q96H22
RRID: AB_2839539

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)
克隆:
Polyclonal
特异性:
CENPN Antibody detects endogenous levels of total CENPN.
RRID:
AB_2839539
引用格式: Affinity Biosciences Cat# DF2315, RRID:AB_2839539.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BM 039; BM 309; BM-309; BM039; C16orf60; CENP N; CENP-N; Cenpn; CENPN_HUMAN; Centromere protein N; FLJ13607; FLJ22660; ICEN 32; ICEN32; Interphase centromere complex protein 32;

抗原和靶标

免疫原:
Uniprot:

Q96H22(CENPN_HUMAN) >>Visit HPA database.

基因/基因ID:
CENPN(55839)
描述:
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
序列:
MDETVAEFIKRTILKIPMNELTTILKAWDFLSENQLQTVNFRQRKESVVQHLIHLCEEKRASISDAALLDIIYMQFHQHQKVWEVFQMSKGPGEDVDLFDMKQFKNSFKKILQRALKNVTVSFRETEENAVWIRIAWGTQYTKPNQYKPTYVVYYSQTPYAFTSSSMLRRNTPLLGQALTIASKHHQIVKMDLRSRYLDSLKAIVFKQYNQTFETHNSTTPLQERSLGLDINMDSRIIHENIVEKERVQRITQETFGDYPQPQLEFAQYKLETKFKSGLNGSILAEREEPLRCLIKFSSPHLLEALKSLAPAGIADAPLSPLLTCIPNKRMNYFKIRDK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
92
Chicken
92
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.

细胞定位:

Nucleus. Chromosome>Centromere>Kinetochore.
Note: Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts directly with CENPA. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1.

蛋白家族:

Belongs to the CENP-N/CHL4 family.

文献引用

1). Dynamic phosphorylation of CENP-N by CDK1 guides accurate chromosome segregation in mitosis. Journal of Molecular Cell Biology, 2022 (PubMed: 37365681) [IF=5.3]

Application: WB    Species: Human    Sample:

Figure 1 The interaction between CENP-L and CENP-N is required for accurate mitotic progression. (A) The binding interface between CENP-L and CENP-N. A close-up view of the key β-strand of CENP-N at the CENP-L–CENP-N interface is shown in magenta. (B) Immunoprecipitation analyses show that the TAT-CENP-N271–300 peptide disrupts the interaction between CENP-L and CENP-N. (C) Disrupting the CENP-L–CENP-N interaction causes chromosome misalignment. Representative images illustrating mitotic progression in mCherry-H2B-expressing cells treated with scramble control peptide or 5 μM TAT-CENP-N271–300 peptide in Opti-MEM for 60 min. Live cell imaging analyses were performed under a DeltaVision microscope. Images were acquired at the indicated time points after the start of nuclear envelope breakdown (control: n = 25, TAT-CENP-N271–300: n = 34). Scale bar, 10 μm. (D) Bar graphs illustrating the percentages of various phenotypes of cells treated as in C.
2). CENPN Acts as a Novel Biomarker that Correlates With the Malignant Phenotypes of Glioma Cells. Frontiers in Genetics, 2021 (PubMed: 34646306) [IF=3.7]

Application: WB    Species: Human    Sample:

FIGURE 6 Effects of CENPN knockdown on glioma cells in vitro. (A,B) Expression of CENPN detected after transfection with siRNA by PCR and western blot. *p < 0.05, **p < 0.01, ***p < 0.001. (C) CCK-8 assay was used to estimate the effect of CENPN knockdown on cell proliferation. *p < 0.05, **p < 0.01, ***p < 0.001, siCENPN-i vs siScr group; #p < 0.05, ##p < 0.01, ###p < 0.001, siCENPN-ii vs siScr group. (D) Cell proliferation was estimated by colony formation assay. (E) Wound-healing assay was used to analyze the migration ability of LN229 and U251 cells. Scale bar, 400 μm. (F,G) Invasive ability and the degree of apoptosis were estimated by Transwell assay (F) and FITC annexin V (G), respectively. Data are the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. Scale bar, 200 μm.
3). Centromere protein N promotes lung adenocarcinoma progression by activating PI3K/AKT signaling pathway. Genes & Genomics, 2022 (PubMed: 35150399) [IF=1.6]

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