CENPF Antibody - #DF2310

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产品: CENPF 抗体
货号: DF2310
描述: Rabbit polyclonal antibody to CENPF
应用: WB IHC
文献验证: WB
反应: Human, Mouse
预测: Pig
蛋白号: P49454
RRID: AB_2839534

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 50ul RMB¥ 1250 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
CENPF Antibody detects endogenous levels of total CENPF.
RRID:
AB_2839534
引用格式: Affinity Biosciences Cat# DF2310, RRID:AB_2839534.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AH antigen; Cell cycle dependent 350K nuclear protein; CENF; CENP F; CENP F kinetochore protein; CENP-F; CENPF; CENPF kinetochore protein; CENPF_HUMAN; Centromere protein F 350/400ka; Centromere protein F; Centromere protein F, 350/400kDa; CILD31; Hcp 1; Hcp1; Kinetochore protein CENP F; Kinetochore protein CENPF; Mitosin; PRO1779; STROMS;

抗原和靶标

免疫原:

A synthesized peptide derived from human CENPF, corresponding to a region within C-terminal amino acids.

基因/基因ID:
CENPF(1063)
描述:
Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia

文献引用

1). The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer. International Journal of Biological Sciences, 2020 (PubMed: 32210727) [IF=8.2]

Application: WB    Species: human    Sample: MCF-7, T47D, MDA-MB-231 and MDA-MB-231/ADR cells

Figure 5.| CENPF-mediated transcriptional regulation of CHK1 by ADR.K After transfection with CENPF-siRNA-1 or CENPF-siRNA-2, CHK1 protein was analyzed by western blot. In MCF-7, T47D, MDA-MB-231 and MDA-MB-231/ADR cells, CENPF inhibition caused significant downregulation of CHK1. Additionally, with MDA-MB-231 exposed to ADR, silencing CENPF also led to a reduction in CHK1 protein. Data shown represent the means (± SD) of three independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001; NS, not significant
2). Long noncoding RNA SH3PXD2A-AS1 promotes NSCLC proliferation and accelerates cell cycle progression by interacting with DHX9. Cell death discovery, 2022 (PubMed: 35410446) [IF=7.0]
3). CENPF knockdown inhibits adriamycin chemoresistance in triple-negative breast cancer via the Rb-E2F1 axis. Scientific Reports, 2023 (PubMed: 36720923) [IF=3.8]

Application: WB    Species: Human    Sample: MDA-MB-231 and MDA-MB-231/ADR cells

Figure 3 CENPF regulates ADR-induced G2/M phase arrest through Chk1. (A) Flow cytometry was performed to determine the effects of CENPF inhibition on cell cycle distribution with or without adriamycin in MDA-MB-231 cells. (B,C) The effect of CENPF inhibition on cell proliferation in MDA-MB-231 (B) or MDA-MB-231/ADR (C) cells. (D) Cell proliferation was determined by EdU assay. (E,F) Using RT‒qPCR, the mRNA level of CHK1 was detected in MDA-MB-231 (E) or MDA-MB-231/ADR (F) cells with CENPF knockdown. (G) Western blot analysis of the effect of CENPF knockdown on Chk1 expression and phosphorylation in MDA-MB-231 and MDA-MB-231/ADR cells exposed to adriamycin. Data shown represent the means (± SD) of three independent experiments; **P 

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