CENPF Antibody - #DF2310

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产品: CENPF 抗体
货号: DF2310
描述: Rabbit polyclonal antibody to CENPF
应用: WB IHC
文献验证: WB
反应: Human, Mouse
预测: Pig
蛋白号: P49454
RRID: AB_2839534

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
CENPF Antibody detects endogenous levels of total CENPF.
RRID:
AB_2839534
引用格式: Affinity Biosciences Cat# DF2310, RRID:AB_2839534.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AH antigen; Cell cycle dependent 350K nuclear protein; CENF; CENP F; CENP F kinetochore protein; CENP-F; CENPF; CENPF kinetochore protein; CENPF_HUMAN; Centromere protein F 350/400ka; Centromere protein F; Centromere protein F, 350/400kDa; CILD31; Hcp 1; Hcp1; Kinetochore protein CENP F; Kinetochore protein CENPF; Mitosin; PRO1779; STROMS;

抗原和靶标

免疫原:

A synthesized peptide derived from human CENPF, corresponding to a region within C-terminal amino acids.

基因/基因ID:
CENPF(1063)
描述:
Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia

文献引用

1). The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer. International Journal of Biological Sciences, 2020 (PubMed: 32210727) [IF=8.2]

Application: WB    Species: human    Sample: MCF-7, T47D, MDA-MB-231 and MDA-MB-231/ADR cells

Figure 5.| CENPF-mediated transcriptional regulation of CHK1 by ADR.K After transfection with CENPF-siRNA-1 or CENPF-siRNA-2, CHK1 protein was analyzed by western blot. In MCF-7, T47D, MDA-MB-231 and MDA-MB-231/ADR cells, CENPF inhibition caused significant downregulation of CHK1. Additionally, with MDA-MB-231 exposed to ADR, silencing CENPF also led to a reduction in CHK1 protein. Data shown represent the means (± SD) of three independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001; NS, not significant
2). Long noncoding RNA SH3PXD2A-AS1 promotes NSCLC proliferation and accelerates cell cycle progression by interacting with DHX9. Cell death discovery, 2022 (PubMed: 35410446) [IF=7.0]

Application: WB    Species: human    Sample: A549, H1299, H292 and H23 cells

Fig. 3: Identification of cytokine-related genes as probable target genes of SH3PXD2A-AS1 in NSCLC proliferation. A Cluster analysis of expression patterns of genes/transcripts in the selected gene set. The colour in the figure represents the normalised expression value of the gene in each sample. Red represents higher expression of the gene in the sample, and blue represents lower expression. B GO and KEGG enrichment analyses were used to classify the significantly differentially expressed genes of cancer cells. C The significantly differentially expressed genes of H292 cells with SH3PXD2A-AS1 knockdown in sequencing results were verified by qRT-PCR assays. D, E Effect of SH3PXD2A-AS1 OE or KD on the protein expression of FOXM1, KIF20A and CENPF in A549, H1299, H292 and H23 cells, as assessed by Western blottings. Tubulin was used as a reference control. F, G Relative mRNA expression levels of SH3PXD2A-AS1, FOXM1, KIF20A and CENPF in A549, H1299, H292 and H23 cells with SH3PXD2A-AS1 OE or KD. The 18 S gene was used as a reference control. Lnc2(SH3PXD2A-AS1). Data are shown as the mean ± standard deviation from three independent experiments. *P 
3). CENPF knockdown inhibits adriamycin chemoresistance in triple-negative breast cancer via the Rb-E2F1 axis. Scientific Reports, 2023 (PubMed: 36720923) [IF=3.8]

Application: WB    Species: Human    Sample: MDA-MB-231 and MDA-MB-231/ADR cells

Figure 3 CENPF regulates ADR-induced G2/M phase arrest through Chk1. (A) Flow cytometry was performed to determine the effects of CENPF inhibition on cell cycle distribution with or without adriamycin in MDA-MB-231 cells. (B,C) The effect of CENPF inhibition on cell proliferation in MDA-MB-231 (B) or MDA-MB-231/ADR (C) cells. (D) Cell proliferation was determined by EdU assay. (E,F) Using RT‒qPCR, the mRNA level of CHK1 was detected in MDA-MB-231 (E) or MDA-MB-231/ADR (F) cells with CENPF knockdown. (G) Western blot analysis of the effect of CENPF knockdown on Chk1 expression and phosphorylation in MDA-MB-231 and MDA-MB-231/ADR cells exposed to adriamycin. Data shown represent the means (± SD) of three independent experiments; **P 

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