CD109 Antibody - #DF2291

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产品: CD109 抗体
货号: DF2291
描述: Rabbit polyclonal antibody to CD109
应用: WB
文献验证: WB
反应: Human, Mouse
分子量: 162 kDa; 162kD(Calculated).
蛋白号: Q6YHK3
RRID: AB_2839517

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
CD109 Antibody detects endogenous levels of total CD109.
RRID:
AB_2839517
引用格式: Affinity Biosciences Cat# DF2291, RRID:AB_2839517.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

150 KDa TGF beta 1 binding protein; 150 KDa TGF-beta-1-binding protein; 7D1; 8A3; Activated T cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD 109; CD109; CD109 antigen; CD109_HUMAN; CPAMD7; DKFZp762L1111; E123; FLJ38569; FLJ41966; Gov system alloantigens on platelets; p180; Platelet specific Gov antigen; Platelet-specific Gov antigen; r150; RP11 525G3.1;

抗原和靶标

免疫原:
Uniprot:

Q6YHK3(CD109_HUMAN) >>Visit HPA database.

基因/基因ID:
CD109(135228)
表达:
Q6YHK3 CD109_HUMAN:

Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 4 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.

描述:
Modulates negatively TGFB1 signaling in keratinocytes
序列:
MQGPPLLTAAHLLCVCTAALAVAPGPRFLVTAPGIIRPGGNVTIGVELLEHCPSQVTVKAELLKTASNLTVSVLEAEGVFEKGSFKTLTLPSLPLNSADEIYELRVTGRTQDEILFSNSTRLSFETKRISVFIQTDKALYKPKQEVKFRIVTLFSDFKPYKTSLNILIKDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQVNDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL

研究背景

功能:

Modulates negatively TGFB1 signaling in keratinocytes.

翻译修饰:

N-glycosylated.

2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.

细胞定位:

Cell membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 4 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.

亚基结构:

Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.

蛋白家族:

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.

文献引用

1). Aptamer-based CRISPR/Cas12a assay for the ultrasensitive detection of extracellular vesicle proteins. TALANTA, 2021 (PubMed: 33076176) [IF=5.6]

Application: WB    Species: human    Sample: SUNE2 cells

Fig. 5. Evaluation of the specificity of the aptamer-CRISPR/Cas12a assay. a Schematics showing the sequence variation at different positions in CD109-apt. b CRISPR/Cas12a detection of single-strand and double-strand CD109-apt with mismatches at different positions (1–2, 3–4, 5–6, 1–4, 3–6). c Western blot analysis of the level of CD109 in SUNE2 cells after transfection with siNC and siCD109 1#, 2#, and 3#. d Histogram analysis of CD109+ EVs in the supernatant of SUNE2 cells treated with siCD109 1#, 2#, and 3# and siNC. e Histogram analysis of CD109+ EVs in the supernatants from NPC cells (HK1, HONE1, and SUNE2 cells) and normal nasopharyngeal cells (NEPC1 and NEPC2 cells). Data are based on 3 replicates; error bars = standard errors. f Histogram analysis of CD109+ EV and EGFR+ EV levels in sera from NPC patients and healthy controls. Data are based on 3 replicates; error bars = standard errors. NEPC2, NEPC2 Bmi-1.

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