USP1 Antibody - #DF2257

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产品: USP1 抗体
货号: DF2257
描述: Rabbit polyclonal antibody to USP1
应用: WB IHC
反应: Human
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 88 kDa; 88kD(Calculated).
蛋白号: O94782
RRID: AB_2839486

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Bovine(82%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(82%)
克隆:
Polyclonal
特异性:
USP1 Antibody detects endogenous levels of total USP1.
RRID:
AB_2839486
引用格式: Affinity Biosciences Cat# DF2257, RRID:AB_2839486.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Deubiquitinating enzyme 1; hUBP; Ubiquitin carboxyl terminal hydrolase 1; Ubiquitin carboxyl-terminal hydrolase 1; Ubiquitin specific peptidase 1; Ubiquitin specific protease 1; Ubiquitin thioesterase 1; Ubiquitin thiolesterase 1; Ubiquitin-specific-processing protease 1; UBP; UBP1_HUMAN; USP 1; Usp1;

抗原和靶标

免疫原:
Uniprot:

O94782(UBP1_HUMAN) >>Visit HPA database.

基因/基因ID:
USP1(7398)
描述:
Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.
序列:
MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVVPAAQSSPINCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLKKEEVKNVAELPTKVEEIPHPKEEMNGINSIEMDSMRHSEDFKEKLPKGNGKRKSDTEFGNMKKKVKLSKEHQSLEENQRQTRSKRKATSDTLESPPKIIPKYISENESPRPSQKKSRVKINWLKSATKQPSILSKFCSLGKITTNQGVKGQSKENECDPEEDLGKCESDNTTNGCGLESPGNTVTPVNVNEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
91
Rabbit
91
Bovine
82
Dog
82
Xenopus
56
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O94782 作为底物

Site PTM Type Enzyme
S7 Phosphorylation
S13 Phosphorylation
S16 Phosphorylation
T33 Phosphorylation
S42 Phosphorylation
K48 Ubiquitination
S67 Phosphorylation
K73 Ubiquitination
K110 Ubiquitination
K124 Sumoylation
K130 Acetylation
K224 Ubiquitination
K232 Sumoylation
K232 Ubiquitination
S248 Phosphorylation
S273 Phosphorylation
K283 Ubiquitination
K285 Ubiquitination
T307 Phosphorylation
S308 Phosphorylation
T310 Phosphorylation
S313 Phosphorylation P06493 (CDK1)
S323 Phosphorylation
S327 Phosphorylation
K343 Ubiquitination
S350 Phosphorylation
K360 Ubiquitination
K368 Ubiquitination
S398 Phosphorylation
S475 Phosphorylation
K483 Ubiquitination
K503 Ubiquitination
K523 Ubiquitination
T529 Phosphorylation
K610 Ubiquitination
K623 Ubiquitination
K656 Ubiquitination
K675 Ubiquitination
Y678 Phosphorylation
Y681 Phosphorylation
K683 Ubiquitination
K689 Ubiquitination
K716 Acetylation
K716 Ubiquitination
S727 Phosphorylation
K732 Ubiquitination
K741 Ubiquitination
K746 Ubiquitination
K761 Ubiquitination
S768 Phosphorylation
S770 Phosphorylation
S772 Phosphorylation
T774 Phosphorylation
S775 Phosphorylation

研究背景

功能:

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.

翻译修饰:

Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.

Ubiquitinated; leading to its subsequent proteasomal degradation.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with FANCD2 and PCNA. Interacts with WDR48.

蛋白家族:

Belongs to the peptidase C19 family.

研究领域

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

文献引用

1). Inhibition of USP1 ameliorates hypertensive nephropathy through regulating oxidative stress and ferroptosis: A precise treatment via SJB3-019A nanodelivery. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (PubMed: 37949326) [IF=4.9]

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