USP1 Antibody - #DF2257

Figure 4. USP1 inhibition enhanced SCLC sensitivity to NK cell cytotoxicity. (A) Western blotting was performed to assess USP1 expression in NCI-H446 cells following siRNA transfection. (B) The cytotoxicity of NK-92 cells against NCI-H446 cells was measured using LDH assay at different effector–target (E:T) ratios. The unpaired t-test was used to perform statistical analysis (**p < 0.01). (C) Flow cytometry was employed to detect apoptosis in NCI-H446 cells co-cultured with NK-92 cells after siRNA transfection. (D) The apoptosis rate was calculated using the mean and standard deviation. The unpaired t-test was used to perform statistical analysis (ns, not significant; **p < 0.01). (E) Immunofluorescence assay was conducted on NCI-H446 cells after exposure to different doses of ML323 (Scale bar, 10 μm). (F) Western blotting was performed to assess the expression of USP1. (G) Flow cytometry was utilized for apoptosis detection in NCI-H446 cells co-cultured with NK-92 cells after treatment with ML323. (H) The apoptosis rate was calculated using the mean and standard deviation. The unpaired t-test was used to perform statistical analysis (ns, not significant; **p < 0.01).