ZO 1 Antibody - #DF2250

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产品: ZO 1 抗体
货号: DF2250
描述: Rabbit polyclonal antibody to ZO 1
应用: WB
文献验证: WB
反应: Human, Mouse
预测: Horse, Rabbit, Dog, Chicken
蛋白号: Q07157
RRID: AB_2839480

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
ZO 1 Antibody detects endogenous levels of total ZO 1.
RRID:
AB_2839480
引用格式: Affinity Biosciences Cat# DF2250, RRID:AB_2839480.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Tight junction protein 1; Tight junction protein ZO-1; Tight junction protein ZO1; TJP1; zo-1; Zo1; ZO1_HUMAN; Zona occludens 1; Zona occludens 1 protein; Zona occludens protein 1; Zonula occludens 1 protein; Zonula occludens protein 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human TJP1, corresponding to a region within the internal amino acids.

基因/基因ID:
TJP1(7082)
描述:
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions

研究领域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

文献引用

1). Traditional Chinese Medicine formula Dai-Zong-Fang alleviating hepatic steatosis in db/db mice via gut microbiota modulation. Frontiers in pharmacology, 2024 (PubMed: 38327989) [IF=5.6]

Application: WB    Species: Mouse    Sample:

FIGURE 4. DZF protects intestinal barrier and reduces intestinal lipid absorption in db/db mice. (A) Comparison of lipopolysaccharide (LPS) concentrations in serum of db/db mice. (B) H&E staining of small intestine (×100). (C) Representative immunoblot images of ZO-1, Occludin, ApoB48, and CD36 in mouse small intestine. (E,G,I,K) Relative protein expression levels of ZO-1, Occludin, ApoB48, and CD36 in mouse small intestine. (D,F,H,J) Relative mRNA levels of Tjp1, Ocln, Apob, and Cd36. Data are presented as mean ± SD [(A–K): n = 6]. #p < 0.05, ##p < 0.01 vs. db/m group; *p < 0.05, **p < 0.01 vs. db/db + Veh group.
2). Trans-Anethole Alleviates Subclinical Necro-Haemorrhagic Enteritis-Induced Intestinal Barrier Dysfunction and Intestinal Inflammation in Broilers. Frontiers in Microbiology, 2022 (PubMed: 35387091) [IF=5.2]

Application: WB    Species: Broilers    Sample:

FIGURE 2 The effects of TA on the intestinal barrier function of subclinical NE broilers. (A) Serum DAO activity; (B) serum D-lactate concentration; (C,D) the mRNA abundance of jejunal and ileal OCLN, ZO-1, and CLDN-1; (E) the protein levels of jejunal OCLN, ZO-1, and CLDN-1; (F) the protein levels of ileal OCLN, ZO-1, and CLDN-1. Values were presented as mean with standard error represented by vertical bars. a,bMeans with different letters differ significantly among the groups (P < 0.05). OCLN, occludin; ZO-1, zonula occludens-1; CLDN-1, claudin-1.
3). Trans-anethole ameliorates LPS-induced inflammation via suppression of TLR4/NF-κB pathway in IEC-6 cells. International Immunopharmacology, 2022 (PubMed: 35617845) [IF=4.8]
4). Heterophyllin B an Active Cyclopeptide Alleviates Dextran Sulfate Sodium-induced Colitis by Modulating Gut Microbiota and Repairing Intestinal Mucosal Barrier via AMPK Activation. MOLECULAR NUTRITION & FOOD RESEARCH, 2022 (PubMed: 35796402) [IF=4.5]
5). LDC7559 Exerts Neuroprotective Effects by Inhibiting GSDMD-Dependent Pyroptosis of Microglia in Mice with Traumatic Brain Injury. Journal of neurotrauma, 2023 (PubMed: 35920115) [IF=3.9]
6). Protective effects of sodium humate and its zinc and selenium chelate on the oxidative stress, inflammatory, and intestinal barrier damage of Salmonella Typhimurium-challenged broiler chickens. Poultry science, 2024 (PubMed: 38471228) [IF=3.8]

Application: WB    Species: chickens    Sample:

Figure 2 Effects of dietary HNa and Zn/Se-HNa supplementation on the intestinal barrier function. (A) Serum DAO activity; (B) serum D-lactate concentration; (C and D) the mRNA abundance of jejunal and ileal OCLN, ZO-1, and CLDN-1; (E and F) the relative protein abundance of jejunal and ileal OCLN, ZO-1, and CLDN-1. Values were expressed as mean with standard error represented by vertical bars. a−c Means with different letters differ significantly among the groups (P < 0.05). OCLN, occludin; ZO-1, zonula occludens-1; CLDN-1, claudin-1.
7). Influence of α-Linolenic Acid on the Intestinal Barrier Integrity and Intestinal Antioxidant Status in Broilers. Food science & nutrition, 2025 (PubMed: 40438093) [IF=3.5]
8). Electromagnetic pulse induced blood-brain barrier breakdown through tight junction opening in rats. Bioelectromagnetics, 2023 (PubMed: 38105659) [IF=1.8]

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