ZO 1 Antibody - #DF2250

FIGURE 4. DZF protects intestinal barrier and reduces intestinal lipid absorption in db/db mice. (A) Comparison of lipopolysaccharide (LPS) concentrations in serum of db/db mice. (B) H&E staining of small intestine (×100). (C) Representative immunoblot images of ZO-1, Occludin, ApoB48, and CD36 in mouse small intestine. (E,G,I,K) Relative protein expression levels of ZO-1, Occludin, ApoB48, and CD36 in mouse small intestine. (D,F,H,J) Relative mRNA levels of Tjp1, Ocln, Apob, and Cd36. Data are presented as mean ± SD [(A–K): n = 6]. #p < 0.05, ##p < 0.01 vs. db/m group; *p < 0.05, **p < 0.01 vs. db/db + Veh group.

FIGURE 2 The effects of TA on the intestinal barrier function of subclinical NE broilers. (A) Serum DAO activity; (B) serum D-lactate concentration; (C,D) the mRNA abundance of jejunal and ileal OCLN, ZO-1, and CLDN-1; (E) the protein levels of jejunal OCLN, ZO-1, and CLDN-1; (F) the protein levels of ileal OCLN, ZO-1, and CLDN-1. Values were presented as mean with standard error represented by vertical bars. a,bMeans with different letters differ significantly among the groups (P < 0.05). OCLN, occludin; ZO-1, zonula occludens-1; CLDN-1, claudin-1.

Figure 2 Effects of dietary HNa and Zn/Se-HNa supplementation on the intestinal barrier function. (A) Serum DAO activity; (B) serum D-lactate concentration; (C and D) the mRNA abundance of jejunal and ileal OCLN, ZO-1, and CLDN-1; (E and F) the relative protein abundance of jejunal and ileal OCLN, ZO-1, and CLDN-1. Values were expressed as mean with standard error represented by vertical bars. a−c Means with different letters differ significantly among the groups (P < 0.05). OCLN, occludin; ZO-1, zonula occludens-1; CLDN-1, claudin-1.

FIGURE 2 Influence of ALA on the relative protein expression of ZO-1 and occludin in the jejunum and ileum of broilers. Values were presented as mean with standard errors appearing as vertical bars. a,bMeans with different letters differ significantly among the groups (p