ZO 1 Antibody - #DF2250

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产品: ZO 1 抗体
货号: DF2250
描述: Rabbit polyclonal antibody to ZO 1
应用: WB
文献验证: WB
反应: Human, Mouse
预测: Horse, Rabbit, Dog, Chicken
分子量: 240 kDa; 195kD(Calculated).
蛋白号: Q07157
RRID: AB_2839480

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
ZO 1 Antibody detects endogenous levels of total ZO 1.
RRID:
AB_2839480
引用格式: Affinity Biosciences Cat# DF2250, RRID:AB_2839480.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Tight junction protein 1; Tight junction protein ZO-1; Tight junction protein ZO1; TJP1; zo-1; Zo1; ZO1_HUMAN; Zona occludens 1; Zona occludens 1 protein; Zona occludens protein 1; Zonula occludens 1 protein; Zonula occludens protein 1;

抗原和靶标

免疫原:
Uniprot:

Q07157(ZO1_HUMAN) >>Visit HPA database.

基因/基因ID:
TJP1(7082)
表达:
Q07157 ZO1_HUMAN:

The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

描述:
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions
序列:
MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Chicken
100
Rabbit
100
Pig
75
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q07157 作为底物

Site PTM Type Enzyme
T16 Phosphorylation
S53 Phosphorylation
S117 Phosphorylation
S125 Phosphorylation
S131 Phosphorylation
Y132 Phosphorylation
S141 Phosphorylation
S144 Phosphorylation
S151 Phosphorylation
K153 Ubiquitination
S160 Phosphorylation
S162 Phosphorylation
S166 Phosphorylation
S168 Phosphorylation
S175 Phosphorylation
S178 Phosphorylation
S179 Phosphorylation
K183 Ubiquitination
T185 Phosphorylation
K194 Ubiquitination
S212 Phosphorylation
S215 Phosphorylation
S241 Phosphorylation
T267 Phosphorylation
S275 Phosphorylation
S277 Phosphorylation
S280 Phosphorylation
S284 Phosphorylation
S290 Phosphorylation
S294 Phosphorylation
S297 Phosphorylation
S300 Phosphorylation
S313 Phosphorylation
S315 Phosphorylation
S320 Phosphorylation
S323 Phosphorylation
S329 Phosphorylation
S334 Phosphorylation
S337 Phosphorylation
S340 Phosphorylation
S347 Phosphorylation
K348 Ubiquitination
S353 Phosphorylation
T354 Phosphorylation
T363 Phosphorylation
T379 Phosphorylation
S381 Phosphorylation
Y389 Phosphorylation
S402 Phosphorylation
S421 Phosphorylation
S432 Phosphorylation
T476 Phosphorylation
S513 Phosphorylation
Y520 Phosphorylation
T548 Phosphorylation
Y550 Phosphorylation
K553 Ubiquitination
S585 Phosphorylation
Y588 Phosphorylation
T589 Phosphorylation
K592 Ubiquitination
S617 Phosphorylation
S622 Phosphorylation
T628 Phosphorylation
Y633 Phosphorylation
T648 Phosphorylation
Y669 Phosphorylation
K673 Ubiquitination
S686 Phosphorylation
K695 Ubiquitination
K702 Ubiquitination
T709 Phosphorylation
Y718 Phosphorylation
K733 Acetylation
K737 Acetylation
T738 Phosphorylation
T770 Phosphorylation
T772 Phosphorylation
T809 Phosphorylation
S810 Phosphorylation
S821 Phosphorylation
Y822 Phosphorylation
S824 Phosphorylation
S828 Phosphorylation
Y830 Phosphorylation
S831 Phosphorylation
Y833 Phosphorylation
S834 Phosphorylation
S837 Phosphorylation
Y843 Phosphorylation
T854 Phosphorylation
T861 Phosphorylation
T868 Phosphorylation
S877 Phosphorylation
S886 Phosphorylation
T894 Phosphorylation
Y895 Phosphorylation
Y898 Phosphorylation
S899 Phosphorylation
S912 Phosphorylation
S919 Phosphorylation
S926 Phosphorylation
S927 Phosphorylation
Y931 Phosphorylation
S933 Phosphorylation
T936 Phosphorylation
S964 Phosphorylation
T965 Phosphorylation
S966 Phosphorylation
Y967 Phosphorylation
S968 Phosphorylation
S973 Phosphorylation
T976 Phosphorylation
S978 Phosphorylation
T979 Phosphorylation
S992 Phosphorylation
S994 Phosphorylation
S1025 Phosphorylation
Y1039 Phosphorylation
S1051 Phosphorylation
T1058 Phosphorylation
Y1059 Phosphorylation
Y1061 Phosphorylation
S1064 Phosphorylation
S1065 Phosphorylation
Y1066 Phosphorylation
T1067 Phosphorylation
S1071 Phosphorylation
Y1074 Phosphorylation
Y1087 Phosphorylation
S1092 Phosphorylation
S1102 Phosphorylation
S1111 Phosphorylation
Y1128 Phosphorylation
S1139 Phosphorylation
Y1140 Phosphorylation
S1142 Phosphorylation
Y1146 Phosphorylation
S1153 Phosphorylation
Y1165 Phosphorylation
T1167 Phosphorylation
S1180 Phosphorylation
K1189 Ubiquitination
Y1191 Phosphorylation
Y1195 Phosphorylation
S1196 Phosphorylation
S1198 Phosphorylation
Y1199 Phosphorylation
K1269 Ubiquitination
S1278 Phosphorylation
K1321 Ubiquitination
T1322 Phosphorylation
Y1324 Phosphorylation
K1331 Ubiquitination
Y1346 Phosphorylation
Y1354 Phosphorylation
Y1355 Phosphorylation
K1357 Ubiquitination
Y1361 Phosphorylation
S1366 Phosphorylation
S1397 Phosphorylation
Y1398 Phosphorylation
S1399 Phosphorylation
S1413 Phosphorylation
K1417 Acetylation
Y1419 Phosphorylation
T1425 Phosphorylation
S1433 Phosphorylation
Y1435 Phosphorylation
S1439 Phosphorylation
T1443 Phosphorylation
S1444 Phosphorylation
S1446 Phosphorylation
S1451 Phosphorylation
S1503 Phosphorylation
T1521 Phosphorylation
Y1524 Phosphorylation
T1528 Phosphorylation
K1530 Methylation
Y1532 Phosphorylation
S1534 Phosphorylation
S1545 Phosphorylation
T1567 Phosphorylation
T1569 Phosphorylation
S1570 Phosphorylation
T1573 Phosphorylation
S1577 Phosphorylation
S1579 Phosphorylation
S1588 Phosphorylation
S1594 Phosphorylation
K1601 Ubiquitination
Y1602 Phosphorylation
S1617 Phosphorylation
S1619 Phosphorylation
S1655 Phosphorylation
K1687 Ubiquitination
K1709 Ubiquitination

研究背景

功能:

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates to the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity).

翻译修饰:

Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ.

细胞定位:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Tight junction. Cell junction. Cell junction>Gap junction. Cell projection>Podosome.
Note: Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact (PubMed:7798316). At podosomal sites, is predominantly localized in the ring structure surrounding the actin core (PubMed:20930113).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

亚基结构:

Homodimer. Forms heterodimers (via the PDZ2 domain) with TJP2 and TJP3. Interacts with OCLN, CALM, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with CDC42BPB and MYZAP. Interacts (via PDZ domain) with GJA1. Interacts (via PDZ domains) with ANKRD2. Interacts with BVES (via the C-terminus cytoplasmic tail) (By similarity). Interacts with HSPA4 and KIRREL1 (By similarity). Interacts with DLL1 (By similarity). Interacts with USP53 (via the C-terminal region) (By similarity). Interacts (via ABR region) with F-actin. Interacts with DNMBP (via C-terminal domain); required for the apical cell-cell junction localization of DNMBP.

蛋白家族:

The 244-aa domain between residues 633 and 876 is the primary occludin (OCLN)-binding site and is required for stable association with the tight junction (PubMed:9792688).

The C-terminal region (residues 1151-1372) is an actin-binding region (ABR) that interacts directly with F-actin and plays an important role in the localization of TJP1 at junctions (PubMed:9792688, PubMed:12354695, PubMed:20930113). The ABR is also required for the localization to puncta at the free edge of cells before initiation of cell-cell contact (PubMed:12354695). The ABR is also necessary for TJP1 recruitment to podosomes (PubMed:20930113).

The second PDZ domain (PDZ2) mediates homodimerization and heterodimerization with TJP2 and TJP3 (PubMed:9792688, PubMed:17928286).

Belongs to the MAGUK family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

文献引用

1). Trans-Anethole Alleviates Subclinical Necro-Haemorrhagic Enteritis-Induced Intestinal Barrier Dysfunction and Intestinal Inflammation in Broilers. Frontiers in Microbiology, 2022 (PubMed: 35387091) [IF=5.2]

Application: WB    Species: Broilers    Sample:

FIGURE 2 The effects of TA on the intestinal barrier function of subclinical NE broilers. (A) Serum DAO activity; (B) serum D-lactate concentration; (C,D) the mRNA abundance of jejunal and ileal OCLN, ZO-1, and CLDN-1; (E) the protein levels of jejunal OCLN, ZO-1, and CLDN-1; (F) the protein levels of ileal OCLN, ZO-1, and CLDN-1. Values were presented as mean with standard error represented by vertical bars. a,bMeans with different letters differ significantly among the groups (P < 0.05). OCLN, occludin; ZO-1, zonula occludens-1; CLDN-1, claudin-1.
2). Trans-anethole ameliorates LPS-induced inflammation via suppression of TLR4/NF-κB pathway in IEC-6 cells. International Immunopharmacology, 2022 (PubMed: 35617845) [IF=4.8]
3). Heterophyllin B an Active Cyclopeptide Alleviates Dextran Sulfate Sodium-induced Colitis by Modulating Gut Microbiota and Repairing Intestinal Mucosal Barrier via AMPK Activation. MOLECULAR NUTRITION & FOOD RESEARCH, 2022 (PubMed: 35796402) [IF=4.5]
4). Protective effects of sodium humate and its zinc and selenium chelate on the oxidative stress, inflammatory, and intestinal barrier damage of Salmonella Typhimurium-challenged broiler chickens. Poultry science, 2024 (PubMed: 38471228) [IF=3.8]

Application: WB    Species: chickens    Sample:

Figure 2 Effects of dietary HNa and Zn/Se-HNa supplementation on the intestinal barrier function. (A) Serum DAO activity; (B) serum D-lactate concentration; (C and D) the mRNA abundance of jejunal and ileal OCLN, ZO-1, and CLDN-1; (E and F) the relative protein abundance of jejunal and ileal OCLN, ZO-1, and CLDN-1. Values were expressed as mean with standard error represented by vertical bars. a−c Means with different letters differ significantly among the groups (P < 0.05). OCLN, occludin; ZO-1, zonula occludens-1; CLDN-1, claudin-1.
5). Electromagnetic pulse induced blood-brain barrier breakdown through tight junction opening in rats. Bioelectromagnetics, 2023 (PubMed: 38105659) [IF=1.8]

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