GAPDH Antibody - #AF7021
(1085)
(228)
产品描述
来源:
Rabbit
应用:
WB 1:3000-1:30000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat, Pig, Bovine, Goat, Monkey, Chicken
克隆:
Polyclonal
特异性:
GAPDH antibody detects endogenous levels of total GAPDH.
RRID:
AB_2839421
引用格式: Affinity Biosciences Cat# AF7021, RRID:AB_2839421.
引用格式: Affinity Biosciences Cat# AF7021, RRID:AB_2839421.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit
抗原和靶标
免疫原:
A synthesized peptide derived from human GAPDH.
基因/基因ID:
描述:
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) iswell known as one of the key enzymes involved in glycolysis.As well as functioning as a glycolytic enzyme in cytoplasm,recent evidence suggests that mammalian GAPDHis also involved in a great number of intracellular procesessuch as membrane fusion, microtubule bundling, phosphotransferaseactivity, nuclear RNA export, DNA replication,and DNA repair.
研究领域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
文献引用
1). Gut microbial alterations in arginine metabolism determine bone mechanical adaptation. Cell metabolism, 2024
(PubMed: 38718794)
[IF=27.7]
2). Immunogenic Material Vaccine for Cancer Immunotherapy by Structure-Dependent Immune Cell Trafficking and Modulation. Advanced materials (Deerfield Beach, Fla.), 2024
(PubMed: 38630978)
[IF=27.4]
3). Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation. Nature Biomedical Engineering, 2019
(PubMed: 31844155)
[IF=26.8]
Application: WB Species: Mice Sample: Tumour cells
Fig. 5 | CNP increases exosome release through HSP–p53–TASP6 signalling pathway. a, Simulated temperature changes at five selected locations.
A 200 V and 10 ms pulse created a localized ‘hot spot’ in the nanochannel outlet with a power density of ~1 × 1014 W m−3
and a peak temperature up to
60 °C from room temperature. Once the pulse ended, the hot spot vanished rapidly due to the extremely small volume of the heated fluid inside the
nanochannel (~1 × 10−12 cm3
) compared with the bulk solution outside the nanochannel (~0.1 cm3
). b, Top-down images of MEFs (green) attaching to the
surface of the CNP device. Red dots show nanochannel locations and room temperature before CNP transfection (0 s). White arrows indicate locations
of the nanochannels. The CNP electric pulse (CNP) sharply increases temperature at the nanochannel–cell surface interface. c, Cross-section view of
nanochannels shows temperature changes in the nanochannels before (0 s), during and after (1 s) a CNP pulse. d, Temperature at the cell–nanochannel
interface transiently (<1 s) increases to ~60 °C. e, Western blot of HSP90 and HSP70 from untreated (PBS) and CNP (with PBS)-stimulated (CNP) MEFs.
f, DLS measurements of exosome concentrations from 108 CNP-stimulated MEFs with or without HSP inhibitors show that HSP70 and HSP90 are critical
for the production of exosomes. NVP-HSP990, HSP90 inhibitor; VER155008, HSP70 inhibitor. g, Western blots show that CNP increases expression of
p53 and TSAP6 protein in p53 wild-type MEFs, but does not affect p53 or TSAP6 protein expression in p53−/− MEFs. h, DLS measurements of exosome
concentrations show that knockdown of p53 can partially block exosome release after CNP. i, Schematic of a proposed mechanism for CNP triggering of
exosome release in CNP-transfected cells. Data are from three independent experiments and are presented as mean ± s.e.m. Two-sided Student’s t-test
was used for the comparison.
4). Circadian Control of Hepatic Ischemia/Reperfusion Injury via HSD17B13-Mediated Autophagy in Hepatocytes. Journal of hepatology, 2025
(PubMed: 40049242)
[IF=26.8]
5). Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription. Nucleic acids research, 2023
(PubMed: 37650635)
[IF=16.6]
6). Glypican-3-targeted macrophages delivering drug-loaded exosomes offer efficient cytotherapy in mouse models of solid tumours. Nature communications, 2024
(PubMed: 39313508)
[IF=16.6]
7). RGS5+ lymphatic endothelial cells facilitate metastasis and acquired drug resistance of breast cancer through oxidative stress-sensing mechanism. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 2024
(PubMed: 39306871)
[IF=15.8]
8). Macrophage-tumor chimeric exosomes accumulate in lymph node and tumor to activate the immune response and the tumor microenvironment. Science Translational Medicine, 2021
(PubMed: 34644149)
[IF=15.8]
9). Peroxisome Deficiency in Cochlear Hair Cells Causes Hearing Loss by Deregulating BK Channels. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023
(PubMed: 37171794)
[IF=15.1]
10). Tailoring Tumor Cell Golgi Apparatus-Targeting Self-Assembled Peptide for Effective Immunotherapy via Reshaping MIF-Mediated Immunosuppressive Network. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025
(PubMed: 39908165)
[IF=15.1]
限制条款
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