Tubulin beta Antibody - #AF7011

Figure 3.| Oligomerized SAFA Mediates IFNb Transcription(I) HEK293 cells were transfected with indicated plasmids and infected with virus for 6 h, followed by oligomerization assay.

Figure 8 Clec7a interacts with MD2 in the ischemic brain. A) Immunoprecipitates were subjected to SDS-PAGE and silver staining. B) Annotated LC–MS/MS spectra of 150-LGAHLLKIDNSKEFEFIESQTSSHR-174 from Clec7a and 85-IELPKRKEVLCHGHDDDYSFCRALK-109 from MD2 in anti-Clec7a immunoprecipitates from tMCAO mice. C) Normalized binding curve of Clec7a and MD2. The binding curve yielded a Kd of 2.96 ± 1.11 µM. The concentration of Clec7a was kept constant at 50 nM while the MD2 concentration was varied from 10 µM to 1.2 nM. n = 3 per group. D) Endogenous Clec7a was coimmunoprecipitated with MD2 after ischemic stroke. The experiments were repeated independently three times. E) The interaction between Clec7a (cyan) and MD2 (pink). The yellow dashed line indicates hydrogen bonding. F) Representative images and analysis of the ischemic penumbra showing the colocalization of MD2 with PSD95, as indicated by the white arrowheads; scale bars, 5 µm. G) Representative images and analysis of the ischemic penumbra showing the colocalization of MD2 with Syn as indicated by the white arrowheads; scale bars, 5 µm. H) Schematic illustrating the in vitro phagocytosis assay in which primary microglia isolated from Clec7ai∆MG or Clec7afl/fl mice phagocytosed MD2ko/ko or MD2wt/wt synaptosomes tagged with pHrodo. I) Representative images of cultured Clec7ai∆MG or Clec7afl/fl microglia engulfing pHrodo-conjugated synaptosomes (top: engulfed pHrodo; bottom: merged image of Iba-1 and pHrodo). Scale bars, 20 µm. Statistics were derived from 18 slices, n = 6 per group. Mice aged 8–10 weeks were used for the experiments shown in this figure. Significance was calculated using either two-tailed unpaired Student's t-test (F and G) or one-way ANOVA, Tukey's multiple comparisons test (I). Data are presented as mean ± SD. ***p < 0.001.