Tubulin beta Antibody - #AF7011
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		产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7011, RRID:AB_2827688.
展开/折叠
TUBB3, CDCBM, Beta III Tubulin, Class III beta-tubulin, TUBB4, Tubulin, beta 3, Tubulin beta-III, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin, beta 3 class III, CFEOM3A
抗原和靶标
A synthesized peptide derived from human Tubulin beta.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
文献引用
Application: WB Species: human Sample: HEK293 cells
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											![Fig. 1. XAF1 facilitates antiviral immune signaling. (A) Flow chart depicting the process of MAVS-TurboID proximity-based labeling technology to detect interacting proteins. XAF1 was identified as a MAVS-interacting protein by mass spectrometry. (B) Luciferase (Luci) activity in HEK293T cells transiently transfected with 50 ng of the pRL-TK reporter, 50 ng of luciferase reporter driven by promoters of genes encoding IFN-β, empty vector (Vector), or XAF1 together with expression plasmids for Cgas, STING, cGAS+STING, N-MDA5, N-RIG-I, MAVS, or TBK1 for 24 hours. (C and D) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of IFNB1 and ISG15 was performed after NT (nontreated) or after stimulation with SeV, VSV, or HSV-1 for 12 hours in XAF1−/− HT-29 (C) and THP-1 (D) cells. (E and F) 2fTGH-ISRE (IFN-stimulated response element) cells were treated with supernatant from control and XAF1−/− HT-29 (E) and THP-1 (F) cells, which were NT or stimulated with SeV, VSV, or HSV-1 for 12 hours. After 6 hours of treatment of the supernatant, ISRE luciferase activity was measured. (G) Heatmap for selected ISGs in WT and XAF1−/− HT-29 cells treated with or without VSV for 6 hours. (H) Histogram of the combined score (https://david.ncifcrf.gov/tools.jsp) for biological processes for the up-regulated genes in WT versus XAF1−/− HT-29 cells treated with or without VSV for 6 hours. Data are the means ± SEM (n = 3) from three independent experiments; NS, not significant; *P < 0.05 and **P < 0.01 [Student’s t test, (B) to (F)]. Data are representative of two [(G) and (H)] or three [(B) to (F)] independent experiments with similar results. Tubulin beta Antibody - Fig.](http://img.affbiotech.cn/uploads/202412/f2c121f800e8ec18fac417d81a307b02.png) 
											 
											 
											 
											 
											