Tubulin alpha Antibody | Affinity Biosciences LTD|亲科生物官网

$Fig. 5. Effects of stigmasterol treatment on inflammatory pathways in Aβ42 oligomers induced BV2 cells when treated with AMPK inhibitor. Cells were pretreated with Compound C (10 μM) for 4 h, and then treated with Aβ42 oligomers (1 μM) for 24 h, followed by treatment with stigmasterol (20 μM) for 4 h. (A,B) Representative western blot analysis of AMPK signaling. GAPDH immunoreactivity was used as a loading control. (C-E) Representative western blot analysis of NF-κB signaling. The cytosolic and nuclear fractions were prepared and analyzed with total NF-κB p65. α-Tubulin immunoreactivity was used as a loading control in the cytosolic fraction, Histone H3 was used as the loading control in the nuclear fraction. (F-H) Representative western blot analysis of NLRP3 signaling, including NLRP3 and Caspase-1, p20. GAPDH immunoreactivity was used as a loading control (I, J) Concentration of TNFα and IL-1β. Data were presented as the mean ± SEM from three independent experiments. One-way ANOVA with Tukey’s multiple comparison test revealed a difference between groups.$

Tubulin alpha Antibody - Fig.

Tubulin alpha Antibody - Figure 4 Western blot of CD68, COL I, vimentin, and α-SMA in male and female mice exposed to silica for two, four, six weeks, and the control groups.

$Figure 2: Distribution of ATP7B and ATP7A in hepatic and non-hepatic cell lines (A) Immunofluorescence image of ATP7B (green) in Huh7 cell line co-stained with LAMP2 (red) and TGN46 (blue) shows presence of ATP7B on both the compartments, irrespective of cellular copper conditions. The marked area on ‘merge’ panel is enlarged in ‘inset’. The arrowhead shows colocalization between ATP7B52 LAMP2. (B) The PCC between ATP7B-TGN46 and ATP7B-LAMP2 in different copper condition in Huh7 cells are demonstrated by box plot with jitter points. (C) Total fraction of ATP7B present in TGN46 and LAMP2 compartment are demonstrated by box plot with jitter points. The statistical comparison is done w.r.t. basal. Sample size (N) for (B) and (C) are BCS: 45, Basal: 42, Cu100: 25. (D)Immunoblot of cell lysate shows presence of endogenous ATP7B (~165kDa) in different cell lines. α-tubulin (~55kDa) has been used as housekeeping protein. (E) Immunofluorescence image of ATP7B (green) in HeLa cell line co-stained with LAMP2 (red) and TGN46 (blue) shows different localization pattern of ATP7B that hepatocytes. The marked area on ‘merge’ panel is enlarged in ‘inset’. The arrowhead shows colocalization between ATP7B-LAMP2, the asterisk shows colocalization between ATP7B and TGN46. (F) The PCC between ATP7B-TGN46 and ATP7B61 LAMP2 in different copper condition are demonstrated by box plot with jitter points. Sample size (N) for (E) are Basal: 36 Cu: 36. (G) Ectopically expressed mKO-ATP7A (red) in HepG2 co-stained with ATP7B (green) and TGN46 (blue) (top panel) and LAMP2 (green) and TGN46 (blue) (bottom panel) shows distinct localization pattern of ATP7A than ATP7B. The marked area on ‘merge’ panel is enlarged in ‘inset’. Asterisk marks the nucleus of the cells in the bottom panel. (H) Model illustrates hepatocyte-specific copper66 independent, and copper-dependent lysosomal localization of ATP7B. [Scale bar: 5µm.]$

Tubulin alpha Antibody - Figure 2: Distribution of ATP7B and ATP7A in hepatic and non-hepatic cell lines (A) Immunofluorescence image of ATP7B (green) in Huh7 cell line co-stained with LAMP2 (red) and TGN46 (blue) shows presence of ATP7B on both the compartments, irrespective of cellular copper conditions.

Tubulin alpha Antibody - Figure 3.

Tubulin alpha Antibody - Figure 5 Effects of liraglutide in MC3T3 cells.

Tubulin alpha Antibody - Fig.

Tubulin alpha Antibody - Figure 2: Variation of protein levels normalized with α-tubulin in cell lines using western blot band images.

Tubulin alpha Antibody - Figure 4 Cu overload destroys embryonic HSPC cytoskeleton (A) Schema for the experiments in (B) and (C).

Tubulin alpha Antibody - Figure 3 Gal-8-LILRB4 interaction activates STAT3 and inhibits NF-κB pathway (A) Immune blotting of 3 potential protein tyrosine phosphatases (PTPs) downstream of LILRB4.

Tubulin alpha Antibody - Figure 5.

产品:	Tubulin alpha 抗体
货号:	AF7010
描述:	Rabbit polyclonal antibody to Tubulin alpha
应用:	WB IHC IF/ICC
文献验证:	WB, IF/ICC
反应:	Human, Mouse, Rat, Pig, Bovine, Rabbit, Chicken, Plants, Fish
预测:	Pig, Bovine, Horse, Sheep, Dog
蛋白号:	P68363 \| Q71U36
RRID:	AB_2839418

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 450	现货
100ul	RMB¥ 750	现货
200ul	RMB¥ 1000	现货

货期: 当天发货

联系销售

MSDS

说明书

COA

实验方案

WB Handbook

IHC Protocol

IF/ICC Protocol

产品描述

来源:

Rabbit

应用:

WB 1:5000-1:20000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human, Mouse, Rat, Pig, Bovine, Rabbit, Chicken, Plants, Fish

克隆:

Polyclonal

特异性:

Tubulin alpha Antibody detects endogenous levels of total Tubulin alpha.

RRID:

AB_2839418
引用格式: Affinity Biosciences Cat# AF7010, RRID:AB_2839418.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

TUBA1A; Alpha-tubulin 3; B-ALPHA-1; Hum-a-tub1; TUBA3; Tubulin alpha-3 chain; Tubulin B-alpha-1; Tubulin alpha 1a; Tubulin alpha; Tubulin Alpha 1a; Tubulin alpha-1A chain;Tubulin alpha-1B chain;

抗原和靶标

免疫原:

A synthesized peptide derived from human Tubulin alpha.

基因/基因ID:

TUBA1B(10376) | TUBA1A(7846)

描述:

TUBA1B Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family

研究领域

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

文献引用

1). Psychologic Stress Drives Progression of Malignant Tumors via DRD2/HIF1α Signaling. CANCER RESEARCH, 2021 (PubMed: 34321238) [IF=12.5]

Application: WB Species: mouse Sample: tumor cells

Fig. 2. |DRD2 overexpression promotes the metastasis, invasion, tube formation, and cloning formation of tumor cells.J, Effect of DRD2 on the expression E-cadherin and vimentin detected by Western blot.

2). Silica Perturbs Primary Cilia and Causes Myofibroblast Differentiation during Silicosis by Reduction of the KIF3A-Repressor GLI3 Complex. Theranostics, 2023 (PubMed: 32042332) [IF=12.4]

Application: WB Species: human Sample: MRC-5 fibroblasts

Figure 5. KIF3A knockdown increases α-SMA-positive myofibroblasts among SiO2-activated MRC-5 fibroblasts. (A) Western blot showing the effects of NC-siRNA and KIF3A-siRNA on expression of KIF3A, Ac-α-Tub, and ARL13B proteins in MRC-5 fibroblasts. GAPDH was used as a loading control (n=3). (B) Densitometric analyses of KIF3A, Ac-α-Tub, and ARL13B protein expression in MRC-5 fibroblasts. *P<0.05; **P<0.01. Data are the mean±SD. Statistical analysis was performed using one-way ANOVA and SPSS 20.0. (C) IF assay showing primary cilia in MRC-5 fibroblasts treated with NC-siRNA or KIF3A-siRNA. Primary cilia were labelled with an anti-Ac-α-Tub antibody. Scale bar=25 and 5 μm. (D) Treatment regimen of KIF3A knockdown in MRC-5 fibroblasts. MRC-5 fibroblasts were stimulated with SiO2 or serum-free medium (n=3 per group) for 12 h, and then transfected with NC-siRNA or KIF3A-siRNA until 36 h. (E) Expression of α-SMA in MRC-5 fibroblasts measured by IF. Scale bar=100 μm. (F, G) Western blot and densitometric analyses of the effects of NC-siRNA and KIF3A-siRNA on expression of COL I, α-SMA, MRTF-A and SRF proteins in MRC-5 fibroblasts with or without SiO2 stimulation. α-Tub was used as a loading control (n=3). *P<0.05; **P<0.01. Data are the mean±SD. Statistical analysis was performed using one-way ANOVA and SPSS 20.0.

3). 2,3′,4,4′,5-Pentachlorobiphenyl induces mitochondria-dependent apoptosis mediated by AhR/Cyp1a1 in mouse germ cells. Journal of Hazardous Materials, 2023 (PubMed: 37055962) [IF=12.2]

4). Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors. Cell reports. Medicine, 2024 (PubMed: 38232701) [IF=11.7]

Application: WB Species: Human Sample: THP-1 cells

Figure 3 Gal-8-LILRB4 interaction activates STAT3 and inhibits NF-κB pathway (A) Immune blotting of 3 potential protein tyrosine phosphatases (PTPs) downstream of LILRB4. Among the 3 PTPs, the phosphorylation level of SHP1 was significantly affected by Gal-8. The statistical plot shows the pSHP1/SHP ratio. (B and C) Immune blotting demonstrates the phosphorylation level of NF-κB and STAT3 with or without Gal-8 treatment in THP-1 (B) and MV411(C) cells. (D) Immune blotting of nuclear and extranuclear proteins of Vector and LILRB4 KD THP-1 cells. (E) Immune blotting of human CD14+ cells treated with or without Gal-8 for 48 or 72 h. The results were constant with what was observed in THP-1 and MV411 cell lines. (F) Immune blotting of S100A8/9 and SOCS3 in human CD14+ cells treated with or without Gal-8. (G and H) Immune blotting of TRAF6 ubiquitination in THP-1 cells with or without LILRB4 KD (G) and with or without Gal-8 treatment (H). The immune blotting was detected with an anti-K63 Ubi antibody. (I) NF-κB reporter gene signal intensity in THP-1 cells cocultured with Gal-8-overexpressing HEK293 cells or control HEK293 cells for 3 days before reporter signals were detected. (J) Immune blotting of ADAM17 expression alteration in THP-1 cells treated with different concentrations of Gal-8 and in Vector and LILRB4-KD THP-1 cells. Of all the statistical analysis of immune blotting results, data were obtained from 3 biological replicates and represented as mean ± SEM.

5). MCTP controls nucleocytoplasmic partitioning of AUXIN RESPONSE FACTORs during lateral root development. Developmental cell, 2024 (PubMed: 39423818) [IF=10.7]

6). BDH1-mediated LRRC31 regulation dependent on histone lysine β-hydroxybutyrylation to promote lung adenocarcinoma progression. MedComm, 2023 (PubMed: 38098610) [IF=9.9]

7). Sophora japonica flowers and their main phytochemical, rutin, regulate chemically induced murine colitis in association with targeting the NF-κB signaling pathway and gut microbiota. Food Chemistry, 2022 (PubMed: 35691061) [IF=8.5]

8). A marine-derived small molecule induces immunogenic cell death against triple-negative breast cancer through ER stress-CHOP pathway. International Journal of Biological Sciences, 2023 (PubMed: 35541893) [IF=8.2]

9). A novel trans-acting lncRNA of ACTG1 that induces the remodeling of ovarian follicles. International journal of biological macromolecules, 2023 (PubMed: 37276900) [IF=7.7]

10). Magnesium-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells by activating ERK/BMP2/Smads signaling. Stem Cell Research & Therapy, 2019 (PubMed: 31823825) [IF=7.5]

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Tubulin alpha Antibody - #AF7010