beta-Tubulin Antibody - #T0023

Fig. 5: Abnormal MSX1 phase separation underlies cleft palate. A Sequence alignment of MSX1 orthologs in indicated vertebrates assessing the conservation of the PRMT1 methylation sites. B–G Control MO and prmt1 MO zebrafish embryos. B Representative images of phh3 staining in zebrafish embryos (left) and quantification of phh3-positive cells in the ethmoid palate region of zebrafish embryos (right). Scale bars, 100 μm. n = 10 embryos. The white dashed line indicates the ethmoid palate. C Representative fluorescence ventral views of zebrafish embryos. The white solid line indicates the ethmoid palate. Scale bars, 500 μm. D Representative Alcian blue staining images of zebrafish embryos. The red dashed line indicates the ethmoid palate. Scale bars, 500 μm. E Quantification of the cleft palate rates in zebrafish embryos. n = 3 biologically independent experiments. F Representative images of zebrafish embryos expressing GFP-tagged MSX1 at 12 hpf in the ethmoid palate region. Scale bars, 20 μm. G Quantification of the circularity of MSX1 condensates in ethmoid palate region from zebrafish embryos. control: n = 152, prmt1 MO: n = 147 condensates. H–L Control MO, msx1 MO, and msx1 MO + PRMT1 mRNA zebrafish embryos. H Immunofluorescence staining images of phh3 in zebrafish embryos. The white dashed line indicates the ethmoid palate. Scale bars, 100 μm. I Quantitative analysis of phh3-positive cells in the ethmoid palate region in zebrafish embryos. n = 27 embryos. J Fluorescence ventral views of zebrafish embryos. The white solid line indicates the ethmoid palate. Scale bars, 500 μm. K Alcian blue staining images of zebrafish embryos. The red dashed line indicates the ethmoid palate. Scale bars, 500 μm. L Quantification of the cleft palate rates in zebrafish embryos. n = 4 biologically independent experiments. M Representative western blot images of aDMA-MSX1 levels in zebrafish embryos microinjected with control and prmt1 MO. N Representative western blot images of aDMA-MSX1 levels in zebrafish embryos microinjected with MSX1-FL mRNA or its mutants, including R150S and R157S mRNA. O–S Control MO, msx1 MO, msx1 MO + FL mRNA, msx1 MO + R150S mRNA, and msx1 MO + R157S mRNA zebrafish embryos. O Representative immunofluorescence staining images of phh3 staining of zebrafish embryos. The white dashed line indicates the ethmoid palate. Scale bars, 100 μm. P Quantitative of phh3-positive cells in the ethmoid palate region in zebrafish embryos. n = 21 embryos. Q Representative fluorescence ventral views of zebrafish embryos. The white solid line indicates the ethmoid palate. Scale bars, 500 μm. R Representative Alcian blue staining images of zebrafish embryos. The red dashed line represents the ethmoid palate. Scale bars, 500 μm. S Quantification of the cleft palate rates in zebrafish embryos. n = 3 biologically independent experiments. MO: morpholino, hpf: hours post-fertilization. All data in this figure are represented as mean ± SD from at least three biologically independent experiments with similar results. Two-tailed Student’s t-test for (B, E, G, S), One-way ANOVA with Dunnett’s multiple comparisons test for (I, L), and Turkey’s multiple comparisons test for (P). Source data are provided as a Source Data file.

Figure 8.| ALKBH5 upregulates the mRNA stability of YTHDF1 in turn promotes the translation of YAP. (A) m6A-meRIP-qPCR of YTHDF1 in CTL or ALKBH5 OE P1 CMs (***P < 0.001, n = 4). (B) RT-qPCR analysis of YTHDF1 in cultured P1 cardiomyocytes transfected with control or ALKBH5 OE plasmid (**P < 0.01, n = 4). (C) RT-qPCR analysis of YTHDF1 in cultured P1 cardiomyocytes transfected with control siRNA or ALKBH5 siRNAs (*P < 0.05, n = 4). (D) Western blot analysis of ALKBH5 and YTHDF1 in cultured P1 cardiomyocytes transfected with control or ALKBH5 OE plasmid.

Figure 2 CA125 downregulates DKK1 expression in ovarian cancer cells. qPCR (A-B), ELISA (C-D), and Western blot (E-F) analysis of DKK1 mRNA levels in A2780 and OVCAR3 cells treated with CA125 at 0, 0.2. and 0.4 µg/mL for 48 h. The results represent the mean ± SD. *p<0.05, **p<0.01, ****p<0.0001.

Fig. 4 Endothelial PDGF-BB induces pericyte-fibroblast transition and extracellular matrix deposition in vitro. a The PDGF-BB expression levels in bEnd.3 cells treated with 1 mg/ml myelin debris at the indicated time points were measured by ELISA. b, c Western blot analysis (b) and quantification (c) of PDGF-BB in bEnd.3 cells treated as described above in a. d The PDGF-BB expression levels in bEnd.3 cells treated with the indicated concentrations of myelin debris for 72 h were measured by ELISA. e, f Western blot analysis (e) and quantification (f) of PDGF-BB in bEnd.3 cells treated as described above in d. g Experimental schematic diagram of pericyte phenotypic transition induced by culture medium (empty, control), EC-CM treated with PBS, Mye-CM treated with myelin debris, and PDGF-BB. h Immunostaining of PDGFRβ (red), pericyte marker NG2 (green, upper panel), and fibroblast markers FSP1 (green, middle panel) and vimentin (green, lower panel) in primary pericytes treated as in g. i Quantification of the percentage of NG2+, FSP1+, and vimentin+ pericytes. j, k Immunostaining and quantification of extracellular matrix collagen I (green, upper panel) and laminin (green, lower panel) in primary pericytes treated as described in g. The nuclei are stained blue with DAPI. l, m Western blot analysis (l) and quantification (m) of NG2, FSP1, and vimentin in primary pericytes treated as described above. n, o Western blot analysis (n) and quantification (o) of extracellular matrix collagen I and laminin in primary pericytes treated as described above. Scale bar: 25 μm (h and j). Data are expressed as mean ± s.e.m. n = 3 independent cultures. *p < 0.05, **p < 0.01, and ***p < 0.001 versus 0 h, 0 mg/ml or control by one-way ANOVA followed by Tukey’s post hoc test in a, c, d, f, i, k, m, and o