beta-Tubulin Antibody - #T0023

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产品: beta-Tubulin 抗体
货号: T0023
描述: Mouse monoclonal antibody to beta-Tubulin
应用: WB IHC IF/ICC ELISA
文献验证: WB
反应: Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken
蛋白号: P07437
RRID: AB_2813772

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   规格 价格 库存
 50ul RMB¥ 450 现货
 100ul RMB¥ 750 现货
 200ul RMB¥ 1000 现货
 1ml RMB¥ 5000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse
应用:
WB 1:10000-1:500000, IHC 1:50-1:200, IF/ICC: 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken
克隆:
Monoclonal [AFfirm039]
特异性:
beta-Tubulin Mouse Monoclonal antibody detects endogenous levels of total beta-Tubulin protein.
RRID:
AB_2813772
引用格式: Affinity Biosciences Cat# T0023, RRID:AB_2813772.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

TUBB3, CDCBM, Beta III Tubulin, Class III beta-tubulin, TUBB4, Tubulin, beta 3, Tubulin beta-III, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin, beta 3 class III, CFEOM3A

抗原和靶标

免疫原:

Full-length beta-tubulin protein of human.

基因/基因ID:
TUBB(203068)
描述:
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells. For example, expression of beta-Tubulin in adipose tissue is very low and therefore beta-Tubulin should not be used as loading control for these tissues.

研究领域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

文献引用

1). PRMT1-methylated MSX1 phase separates to control palate development. Nature communications, 2025 (PubMed: 39843447) [IF=16.6]
2). The clinical antiprotozoal drug nitazoxanide and its metabolite tizoxanide extend Caenorhabditis elegans lifespan and healthspan. Acta pharmaceutica Sinica. B, 2024 (PubMed: 39027239) [IF=14.7]
3). ALKBH5 regulates cardiomyocyte proliferation and heart regeneration by demethylating the mRNA of YTHDF1. Theranostics, 2021 (PubMed: 33456585) [IF=12.4]

Application: WB    Species: mouse    Sample: cardiomyocytes

Figure 8.| ALKBH5 upregulates the mRNA stability of YTHDF1 in turn promotes the translation of YAP. (A) m6A-meRIP-qPCR of YTHDF1 in CTL or ALKBH5 OE P1 CMs (***P < 0.001, n = 4). (B) RT-qPCR analysis of YTHDF1 in cultured P1 cardiomyocytes transfected with control or ALKBH5 OE plasmid (**P < 0.01, n = 4). (C) RT-qPCR analysis of YTHDF1 in cultured P1 cardiomyocytes transfected with control siRNA or ALKBH5 siRNAs (*P < 0.05, n = 4). (D) Western blot analysis of ALKBH5 and YTHDF1 in cultured P1 cardiomyocytes transfected with control or ALKBH5 OE plasmid.
4). Magnetic sculpture-like tumor cell vaccines enable targeted in situ immune activation and potent antitumor effects. Theranostics, 2025 (PubMed: 40303352) [IF=12.4]
5). Exercise-derived peptide protects against pathological cardiac remodeling. EBioMedicine, 2022 (PubMed: 35843176) [IF=9.7]
6). Free CA125 promotes ovarian cancer cell migration and tumor metastasis by binding Mesothelin to reduce DKK1 expression and activate the SGK3/FOXO3 pathway. International Journal of Biological Sciences, 2023 (PubMed: 33613114) [IF=8.2]

Application: WB    Species: Human    Sample: A2780 and OVCAR3 cells

Figure 2 CA125 downregulates DKK1 expression in ovarian cancer cells. qPCR (A-B), ELISA (C-D), and Western blot (E-F) analysis of DKK1 mRNA levels in A2780 and OVCAR3 cells treated with CA125 at 0, 0.2. and 0.4 µg/mL for 48 h. The results represent the mean ± SD. *p<0.05, **p<0.01, ****p<0.0001.
7). EGFR tyrosine kinase activity and Rab GTPases coordinate EGFR trafficking to regulate macrophage activation in sepsis. Cell Death & Disease, 2022 (PubMed: 36344490) [IF=8.1]
8). Imatinib inhibits pericyte-fibroblast transition and inflammation and promotes axon regeneration by blocking the PDGF-BB/PDGFRβ pathway in spinal cord injury. Inflammation and regeneration, 2022 (PubMed: 36163271) [IF=8.1]
9). Knockdown of RUVBL2 improves hnRNPA2/B1-stress granules dynamics to inhibit perioperative neurocognitive disorders in aged mild cognitive impairment rats. Aging cell, 2025 (PubMed: 39610020) [IF=8.0]
10). A bioactive xyloglucan polysaccharide hydrogel mechanically enhanced by Pluronic F127 micelles for promoting chronic wound healing. International journal of biological macromolecules, 2024 (PubMed: 39047998) [IF=7.7]
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