TWEAK Antibody - #DF7444

Fig. 1. CD163 regulates bone homeostasis and its level is reduced in inflammatory bone loss. (A, B) Representative μCT images and quantification of the distal trabecular bone and middle cortical bone of femora from CD163−/− male mice or their WT male littermates. (C, D). Representative images and quantification of TRAP staining from CD163−/− male mice or their WT male littermates. Scale bar: 200 μm (top), 100 μm (bottom). (E–H) Representative images and quantification of NFATc1 and CtsK from CD163−/− male mice or their WT male littermates. Scale bar: 50 μm (top), 10 μm (bottom). (I, J) Representative images and quantification of CD163 from mice treated with or without LPS. Scale bar: 50 μm (top), 10 μm (bottom). (K, L) Representative immunoblotting and quantification of CD163 and TWEAK in the bone marrow contents from mice treated with or without LPS. (M, N) Representative immunoblotting and quantification of CD163, TWEAK, and Fn14 in the bone marrow contents from RA individuals or controls. Dots represent individual mice or human samples.

Fig. 7. GXF interfered the interaction between macrophages and stellate cells. A The mRNA expression of TGFβ and EGFR were evaluated by RT-qPCR. B The protein levels of TGFβ and EGFR. C The mRNA expression of PDGFB and PDGFRα were evaluated by RT-qPCR. D The protein levels of PDGFB and PDGFRα. E The mRNA expression of TNFSF12 and TNFRSF12a were evaluated by RT-qPCR. F The protein levels of TNFSF12 and TNFRSF12a. The data are presented as means ± SEM (n = 4). #p