CEA Antibody - #BF0092

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产品: CEA 抗体
货号: BF0092
描述: Mouse monoclonal antibody to CEA
应用: WB IHC IF/ICC ELISA
反应: Human
分子量: 77kDa; 77kD(Calculated).
蛋白号: P06731
RRID: AB_2833881

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 50ul RMB¥ 1800 现货
 100ul RMB¥ 2800 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse
应用:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFB1857]
特异性:
CEA antibody detects endogenous levels of total CEA.
RRID:
AB_2833881
引用格式: Affinity Biosciences Cat# BF0092, RRID:AB_2833881.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Carcinoembryonic antigen; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; Ceacam5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100; OTTHUMP00000199032; OTTHUMP00000199033; OTTHUMP00000199034;

抗原和靶标

免疫原:

Purified recombinant fragment of human CEA expressed in E. Coli.

Uniprot:

P06731(CEAM5_HUMAN) >>Visit HPA database.

基因/基因ID:
CEACAM5(1048)
表达:
P06731 CEAM5_HUMAN:

Expressed in columnar epithelial and goblet cells of the colon (at protein level) (PubMed:10436421). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

描述:
Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
序列:
MESPSAPPHRWCIPWQRLLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLVHNLPQHLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYTLHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWVNNQSLPVSPRLQLSNGNRTLTLFNVTRNDTASYKCETQNPVSARRSDSVILNVLYGPDAPTISPLNTSYRSGENLNLSCHAASNPPAQYSWFVNGTFQQSTQELFIPNITVNNSGSYTCQAHNSDTGLNRTTVTTITVYAEPPKPFITSNNSNPVEDEDAVALTCEPEIQNTTYLWWVNNQSLPVSPRLQLSNDNRTLTLLSVTRNDVGPYECGIQNKLSVDHSDPVILNVLYGPDDPTISPSYTYYRPGVNLSLSCHAASNPPAQYSWLIDGNIQQHTQELFISNITEKNSGLYTCQANNSASGHSRTTVKTITVSAELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLFNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTPIISPPDSSYLSGANLNLSCHSASNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFVSNLATGRNNSIVKSITVSASGTSPGLSAGATVGIMIGVLVGVALI

翻译修饰 - P06731 作为底物

Site PTM Type Enzyme
T199 Phosphorylation
T201 Phosphorylation
T206 Phosphorylation
Y213 Phosphorylation
S226 Phosphorylation
N246 N-Glycosylation
S404 Phosphorylation
T419 Phosphorylation
S421 Phosphorylation
T476 Phosphorylation
N560 N-Glycosylation
S680 Phosphorylation

研究背景

功能:

Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.

(Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.

翻译修饰:

Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.

细胞定位:

Cell membrane>Lipid-anchor. Apical cell membrane. Cell surface.
Note: Localized to the apical glycocalyx surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in columnar epithelial and goblet cells of the colon (at protein level). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

亚基结构:

Homodimer.

蛋白家族:

Belongs to the immunoglobulin superfamily. CEA family.

文献引用

1). Upregulation of ITGB6 in primary palmar hyperhidrosis. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 2023 (PubMed: 37212774) [IF=2.1]

Application: IF/ICC    Species: Human    Sample:

Fig. 2. Identification of human sweat gland cells and verification of integrin β6 (ITGB6) overexpression transfection efficiency. A. Immunofluorescence staining of CEA (green) and CK7 (red) in sweat gland cells extracted from patients with primary palmar hyperhidrosis. The cell nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (blue); B. Quantitative polymerase chain reaction (ITGB6 compared to control, Tukey’s honest significant difference (HSD), p < 0.001; ITGB6 compared to negative control (NC), Tukey’s HSD, p < 0.001); C,D. Western blot detection of the transfection efficiency of ITGB6 overexpression analysis results (ITGB6 compared to control, Tukey’s HSD, p < 0.001; ITGB6 compared to NC, Tukey’s HSD, p < 0.001). The data are presented as mean ± 95% confidence interval (95% CI)

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