CALCA Antibody - #DF7386

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产品: CALCA 抗体
货号: DF7386
描述: Rabbit polyclonal antibody to CALCA
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Sheep, Dog, Chicken
分子量: 13kDa; 14kD(Calculated).
蛋白号: P06881
RRID: AB_2839324

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(83%), Zebrafish(%), Sheep(%), Dog(%), Chicken(%)
克隆:
Polyclonal
特异性:
CALCA Antibody detects endogenous levels of total CALCA.
RRID:
AB_2839324
引用格式: Affinity Biosciences Cat# DF7386, RRID:AB_2839324.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Calcitonin gene-related peptide 1;Alpha-type CGRP;Calcitonin gene-related peptide I;CGRP-I;CALCA;CALC1;

抗原和靶标

免疫原:

A synthesized peptide derived from human CALCA, corresponding to a region within the internal amino acids.

Uniprot:

P06881(CALCA_HUMAN) >>Visit HPA database.

基因/基因ID:
CALCA(796)
描述:
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
序列:
MGFQKFSPFLALSILVLLQAGSLHAAPFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSRIIAQKRACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAFGRRRRDLQA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
92
Zebrafish
89
Pig
83
Sheep
83
Chicken
83
Horse
0
Bovine
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the calcitonin family.

文献引用

1). Danggui niantong decoction attenuates synovial fibrosis through regulating PI3k/AKT signaling pathway. Journal of ethnopharmacology, 2025 (PubMed: 39832630) [IF=4.8]
2). Activation of oxytocin receptor in the trigeminal ganglion attenuates orofacial ectopic pain attributed to inferior alveolar nerve injury. Journal of Neurophysiology, 2021 (PubMed: 33326336) [IF=2.1]

Application: IF/ICC    Species: Rat    Sample:

Figure 2.Oxytocin receptor (OXTR) wasexpressed in neurons but not glial cells inthe trigeminal ganglion (TG) and spinal tri-geminal nucleus caudalis (SpVc) of inferioralveolar nerve (IAN) transection (IANX)rats.A: in TG slices, arrowheads showcolocalization of NeuN (neuron marker)and OXTR (red, NeuN; green, OXTR; blue,DAPI).B: in TG slices, activated satelliteglial cells (arrows) surrounded OXTR-posi-tive neurons (arrowheads), and there wasno coexpression [red, glialfibrillary acidicprotein (GFAP); green, OXTR; blue, DAPI].C: in TG slices, coexpression of OXTR andcalcitonin gene-related peptide (CGRP) isindicated with arrowheads (red, CGRP;green, OXTR; blue, DAPI).D:inSpVcsli-ces, double immunohistochemistry indi-cates colocalization of NeuN and OXTR(arrowheads: red, NeuN; green, OXTR;blue, DAPI).E:inSpVcslices,immunofluo-rescence (IF) staining shows no OXTR(arrowheads) colocalized with GFAP(arrows: red, GFAP; green, OXTR; blue,DAPI) (n= 3 rats in each group; scale bars,50μm).INFLUENCE OF OXYTOCIN RECEPTOR ON OROFACIAL ECTOPIC PAINJ Neurophysioldoi:10.1152/jn.00646.2020www.jn.org227

Application: WB    Species: Rat    Sample:

Figure 3.Inferior alveolar nerve (IAN) transection (IANX) resulted in upregulated expression of oxytocin receptor (OXTR), calcitonin gene-related peptide(CGRP), IL-1b, and TNFain the trigeminal ganglion (TG) ondays 1, 4,and7after surgery.AandD: representative Western blot (WB) bands of OXTR,CGRP, IL-1b, and TNFaexpression in the trigeminal ganglion (TG) ondays 1, 4,and7after operation.B, C, E,andF: bar graphs show increased OXTR,CGRP, IL-1band TNFaexpression of IANX rats vs. 2 other groups; sham operation did not result in significant change vs. naive rats (P

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