Dnmt1 Antibody - #DF7376

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产品: Dnmt1 抗体
货号: DF7376
描述: Rabbit polyclonal antibody to Dnmt1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep
蛋白号: P26358
RRID: AB_2839314

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Dnmt1 Antibody detects endogenous levels of total Dnmt1.
RRID:
AB_2839314
引用格式: Affinity Biosciences Cat# DF7376, RRID:AB_2839314.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADCADN; AIM; CXXC finger protein 9; CXXC-type zinc finger protein 9; CXXC9; DNA (cytosine 5 ) methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase 1; DNA methyltransferase HsaI; DNA methyltransferase M.HsaI.; DNA MTase; DNA MTase HsaI; DNMT 1; DNMT; Dnmt1; DNMT1_HUMAN; Dnmt1o; FLJ16293; HSN1E; M.HsaI; MCMT; Met1; MGC104992; mMmul; MommeD2;

抗原和靶标

免疫原:

A synthesized peptide derived from human Dnmt1, corresponding to a region within the internal amino acids.

基因/基因ID:
DNMT1(1786)
描述:
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene.

研究领域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Downregulation of Ambra1 by altered DNA methylation exacerbates dopaminergic neuron damage in a fenpropathrin-induced Parkinson-like mouse model. Ecotoxicology and environmental safety, 2024 (PubMed: 38245935) [IF=6.2]
2). Knockdown of DNMT1 Induces SLCO3A1 to Promote Follicular Growth by Enhancing the Proliferation of Granulosa Cells in Mammals. International journal of molecular sciences, 2024 (PubMed: 38473715) [IF=5.6]

Application: WB    Species: Mouse    Sample: GCs

Figure 5. Knockdown of DNMT1 upregulates the mRNA and protein levels of SLCO3A1. (A) RT-qPCR analysis of SLCO3A1 expression in the small (3 mm in diameter) follicles. (B) Western blot analysis of SLCO3A1 in the small and large follicles. (C) RT-qPCR analysis of SLCO3A1 expression in the DMSO or 5-Aza-CdR treated-cells. (D) Western blot analysis of SLCO3A1 in the DMSO or 5-Aza-CdR treated-cells. (E) Chromatin accessibility assay of region-1, region-2, and region-3 in the SLCO3A1 promoter treated with DMSO or 5-Aza-CdR (region-1, +971/+1252 bp, region-2, +1433/+1557 bp, and region-3, +1623/+1831 bp, transcription start site = +2000 bp). (F) RT-qPCR analysis of SLCO3A1 expression in GCs transfected with siRNAs of DNMT1, DNMT3A, or DNMT3B. Western blot analysis of SLCO3A1, DNMT1 (G), DNMT3A (H), and DNMT3B (I) in the siRNAs of DNMT1, DNMT3A, or DNMT3B treated-cells. *** p < 0.001.
3). Gandouling induces GSK3β promoter methylation to improve cognitive impairment in Wilson's disease. Journal of ethnopharmacology, 2024 (PubMed: 38925320) [IF=4.8]
4). Decreased ovarian function and autophagy gene methylation in aging rats. Journal of Ovarian Research, 2020 (PubMed: 32014030) [IF=4.0]

Application: WB    Species: rat    Sample: ovaries

Fig. 6| Protein expression of Dnmt1, Dnmt2, Dnmt3A and Dnmt3B in the ovaries of rats.(a) Western blot results.
5). Tacrolimus Maintains the Balance of Neutrophil Extracellular Traps by Inducing DNA Methylation of Neutrophils to Reduce Immune Rejection. Life (Basel, Switzerland), 2023 (PubMed: 38137854) [IF=3.2]

Application: WB    Species: Human    Sample: HL-60 Cells

Figure 4 The gene expression of DNMTs and TETs in the four groups of cells. (A) Western blot picture of TET1 and DNMT1 in the four groups of cells. Western blot data of (B) DNMT1 and (C) TET1 expression levels. (D–I) The protein content of DNMT1, DNMT3A, DNMT3B, TET1, TET2, TET3, respectively. ns indicates no significant difference.
6). Cissus quadrangularis extract attenuates diabetic nephropathy by altering SIRT1/DNMT1 axis. JOURNAL OF PHARMACY AND PHARMACOLOGY, 2021 (PubMed: 34128987) [IF=2.8]
7). Determination of DNA Methyltransferase 1 in Cells Using a RG108-Fluorescein Conjugate to Monitor the Fluorescent Ratio with a Microplate Reader. ANALYTICAL LETTERS, 2023 [IF=1.6]
8). The expressions of DNA methyltransferase 1 (DNMT1) and cyclin A1 (CCNA1) in cervical carcinogenesis. International Journal of Clinical and Experimental Pathology, 2019 (PubMed: 31933719) [IF=1.1]
9). Prenatal stress increases corticosterone levels in offspring by impairing placental glucocorticoid barrier function. Research Square, 2023

Application: WB    Species: Rat    Sample:

Figure 4. Effects of prenatal stress on offspring enzymes of DNA methylation in placenta. The figure showed that the expressions of DNMT1, DNMT 3A, DNMT 3B, and β-actin were identified by q-PCR and western blot. The levels of DNMT 3A and DNMT 3B were reduced but DNMT1 expression was enhanced by prenatal stress on offspring. Three biological replicates were carried out. DNMT1, DNA Methyltransferase 1; DNMT 3A, DNA Methyltransferase 3A; DNMT 3B, DNA Methyltransferase 3B. All data are shown as mean ± SD. Number of animals in each group = 3. *Statistically significant difference compared with the OPC group (P

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