HADHA Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IHC
IF/ICC

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Western blot analysis of extracts from rat brain, using HADHA Antibody.

DF7288 at 1/100 staining Mouse stomach tissue by IHC-P.

DF7288 staining A549 cells by IF/ICC.

DF7288 staining Hela cells by IF/ICC.

产品:	HADHA 抗体
货号:	DF7288
描述:	Rabbit polyclonal antibody to HADHA
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量:	83kDa; 83kD(Calculated).
蛋白号:	P40939
RRID:	AB_2839227

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)

克隆:

Polyclonal

特异性:

HADHA Antibody detects endogenous levels of total HADHA.

RRID:

AB_2839227
引用格式: Affinity Biosciences Cat# DF7288, RRID:AB_2839227.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

3 ketoacyl Coenzyme A (CoA) thiolase alpha subunit; 3 oxoacyl CoA thiolase; 78 kDa gastrin binding protein; 78 kDa gastrin-binding protein; ECHA; ECHA_HUMAN; GBP; HADH; HADHA; Hydroxyacyl Coenzyme A dehydrogenase/3 ketoacyl Coenzyme A thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit; LCEH; LCHAD; Long chain 3-hydroxyacyl-CoA dehydrogenase; Mitochondrial long chain 2 enoyl Coenzyme A (CoA) hydratase alpha subunit; Mitochondrial long chain L 3 hydroxyacyl Coenzyme A dehydrogenase alpha subunit; Mitochondrial trifunctional enzyme alpha subunit; Mitochondrial trifunctional protein alpha subunit; MTPA; Thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit; TP ALPHA; TP-alpha; Trifunctional enzyme subunit alpha mitochondrial precursor;

抗原和靶标

免疫原:

Uniprot:

P40939(ECHA_HUMAN) >>Visit HPA database.

基因/基因ID:

HADHA(3030)

描述:

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.

序列:

P40939 ECHA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVACRAIGILSRFSAFRILRSRGYICRNFTGSSALLTRTHINYGVKGDVAVVRINSPNSKVNTLSKELHSEFSEVMNEIWASDQIRSAVLISSKPGCFIAGADINMLAACKTLQEVTQLSQEAQRIVEKLEKSTKPIVAAINGSCLGGGLEVAISCQYRIATKDRKTVLGTPEVLLGALPGAGGTQRLPKMVGVPAALDMMLTGRSIRADRAKKMGLVDQLVEPLGPGLKPPEERTIEYLEEVAITFAKGLADKKISPKRDKGLVEKLTAYAMTIPFVRQQVYKKVEEKVRKQTKGLYPAPLKIIDVVKTGIEQGSDAGYLCESQKFGELVMTKESKALMGLYHGQVLCKKNKFGAPQKDVKHLAILGAGLMGAGIAQVSVDKGLKTILKDATLTALDRGQQQVFKGLNDKVKKKALTSFERDSIFSNLTGQLDYQGFEKADMVIEAVFEDLSLKHRVLKEVEAVIPDHCIFASNTSALPISEIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTEKTSKDTSASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDSLTTSFGFPVGAATLVDEVGVDVAKHVAEDLGKVFGERFGGGNPELLTQMVSKGFLGRKSGKGFYIYQEGVKRKDLNSDMDSILASLKLPPKSEVSSDEDIQFRLVTRFVNEAVMCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKIVDRLKKYEAAYGKQFTPCQLLADHANSPNKKFYQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Chicken

100

Rabbit

100

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P40939 作为底物

P40939

Site	PTM Type	Source
K60	Acetylation	Uniprot
K60	Ubiquitination	Uniprot
T117	Phosphorylation	Uniprot
S120	Phosphorylation	Uniprot
K129	Acetylation	Uniprot
K129	Ubiquitination	Uniprot
K163	Acetylation	Uniprot
T167	Phosphorylation	Uniprot
T171	Phosphorylation	Uniprot
T203	Phosphorylation	Uniprot
S206	Phosphorylation	Uniprot
K214	Ubiquitination	Uniprot
Y239	Phosphorylation	Uniprot
K249	Acetylation	Uniprot
S257	Phosphorylation	Uniprot
K259	Acetylation	Uniprot
K285	Acetylation	Uniprot
K289	Acetylation	Uniprot
T294	Phosphorylation	Uniprot
K295	Acetylation	Uniprot
Y298	Phosphorylation	Uniprot
K303	Acetylation	Uniprot
K303	Ubiquitination	Uniprot
K309	Ubiquitination	Uniprot
S316	Phosphorylation	Uniprot
C322	S-Nitrosylation	Uniprot
K326	Acetylation	Uniprot
K334	Acetylation	Uniprot
K350	Acetylation	Uniprot
K350	Ubiquitination	Uniprot
K351	Acetylation	Uniprot
K353	Acetylation	Uniprot
K353	Ubiquitination	Uniprot
K359	Acetylation	Uniprot
K359	Ubiquitination	Uniprot
K383	Acetylation	Uniprot
K383	Ubiquitination	Uniprot
T387	Phosphorylation	Uniprot
K390	Acetylation	Uniprot
K390	Ubiquitination	Uniprot
T393	Phosphorylation	Uniprot
T395	Phosphorylation	Uniprot
R399	Methylation	Uniprot
K406	Acetylation	Uniprot
K406	Ubiquitination	Uniprot
K411	Acetylation	Uniprot
K413	Acetylation	Uniprot
K415	Ubiquitination	Uniprot
T418	Phosphorylation	Uniprot
S419	Phosphorylation	Uniprot
S427	Phosphorylation	Uniprot
Y435	Phosphorylation	Uniprot
S453	Phosphorylation	Uniprot
K460	Acetylation	Uniprot
K489	Ubiquitination	Uniprot
Y499	Phosphorylation	Uniprot
S501	Phosphorylation	Uniprot
K505	Acetylation	Uniprot
K519	Acetylation	Uniprot
K519	Ubiquitination	Uniprot
T521	Phosphorylation	Uniprot
S522	Phosphorylation	Uniprot
S524	Phosphorylation	Uniprot
K531	Ubiquitination	Uniprot
K540	Acetylation	Uniprot
Y546	Phosphorylation	Uniprot
R549	Methylation	Uniprot
K569	Acetylation	Uniprot
K570	Acetylation	Uniprot
K597	Acetylation	Uniprot
K605	Acetylation	Uniprot
K605	Ubiquitination	Uniprot
K625	Ubiquitination	Uniprot
Y637	Phosphorylation	Uniprot
Y639	Phosphorylation	Uniprot
K644	Acetylation	Uniprot
K646	Ubiquitination	Uniprot
S650	Phosphorylation	Uniprot
S665	Phosphorylation	Uniprot
S668	Phosphorylation	Uniprot
S669	Phosphorylation	Uniprot
Y724	Phosphorylation	Uniprot
K728	Acetylation	Uniprot
K728	Ubiquitination	Uniprot
K735	Ubiquitination	Uniprot
K742	Acetylation	Uniprot
K742	Ubiquitination	Uniprot
T745	Phosphorylation	Uniprot
S756	Phosphorylation	Uniprot
K759	Acetylation	Uniprot
K760	Acetylation	Uniprot

研究背景

P40939_ECHA_HUMAN

功能:

Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA described here carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities while the trifunctional enzyme subunit beta/HADHB bears the 3-ketoacyl-CoA thiolase activity. Independently of the subunit beta, the trifunctional enzyme subunit alpha/HADHA also has a monolysocardiolipin acyltransferase activity. It acylates monolysocardiolipin into cardiolipin, a major mitochondrial membrane phospholipid which plays a key role in apoptosis and supports mitochondrial respiratory chain complexes in the generation of ATP. Allows the acylation of monolysocardiolipin with different acyl-CoA substrates including oleoyl-CoA for which it displays the highest activity.

细胞定位:

Mitochondrion. Mitochondrion inner membrane.
Note: Protein stability and association with mitochondrion inner membrane do not require HADHB.

亚基结构:

Heterotetramer of 2 alpha/HADHA and 2 beta/HADHB subunits; forms the mitochondrial trifunctional enzyme. Also purified as higher order heterooligomers including a 4 alpha/HADHA and 4 beta/HADHB heterooligomer which physiological significance remains unclear.

蛋白家族:

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

研究领域

· Metabolism > Lipid metabolism > Fatty acid elongation.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Amino acid metabolism > Lysine degradation.

· Metabolism > Amino acid metabolism > Tryptophan metabolism.

· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Carbohydrate metabolism > Butanoate metabolism.

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Fatty acid metabolism.

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HADHA Antibody - #DF7288

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