DAO Antibody - #DF7266

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产品: DAO 抗体
货号: DF7266
描述: Rabbit polyclonal antibody to DAO
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 39kDa; 39kD(Calculated).
蛋白号: P14920
RRID: AB_2839205

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
DAO Antibody detects endogenous levels of total DAO.
RRID:
AB_2839205
引用格式: Affinity Biosciences Cat# DF7266, RRID:AB_2839205.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

D-amino-acid oxidase; DAAO; DAMOX; DAO; DAO1; EC 1.4.3.3; MGC35381; OXDA; OXDA_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human DAO, corresponding to a region within C-terminal amino acids.

Uniprot:

P14920(OXDA_HUMAN) >>Visit HPA database.

基因/基因ID:
DAO(1610)
描述:
This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia.
序列:
MRVVVIGAGVIGLSTALCIHERYHSVLQPLDIKVYADRFTPLTTTDVAAGLWQPYLSDPNNPQEADWSQQTFDYLLSHVHSPNAENLGLFLISGYNLFHEAIPDPSWKDTVLGFRKLTPRELDMFPDYGYGWFHTSLILEGKNYLQWLTERLTERGVKFFQRKVESFEEVAREGADVIVNCTGVWAGALQRDPLLQPGRGQIMKVDAPWMKHFILTHDPERGIYNSPYIIPGTQTVTLGGIFQLGNWSELNNIQDHNTIWEGCCRLEPTLKNARIIGERTGFRPVRPQIRLEREQLRTGPSNTEVIHNYGHGGYGLTIHWGCALEAAKLFGRILEEKKLSRMPPSHL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Rabbit
100
Bovine
92
Sheep
92
Chicken
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.

细胞定位:

Peroxisome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the DAMOX/DASOX family.

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Metabolism of other amino acids > D-Arginine and D-ornithine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Triiodothyronine acts on DAO to regulate pulmonary fibrosis progression by facilitating cell senescence through the p53/p21 signaling pathway. Frontiers in pharmacology, 2024 (PubMed: 39323641) [IF=5.6]

Application: WB    Species: Mouse    Sample: lungs

FIGURE 1. DAO expression was downregulated in IPF lungs and BLM-induced fibrotic mouse lungs. (A) The results of IPF gene sequencing showed that DAO expression was significantly lower in IPF patients than in the controls; (B) A549 cells treated with 0.02 U/mL of BLM for 24 h. Representative immunoblot image of the whole-cell lysate of the DAO protein expression; (C) Representative immunoblot images (up) and densitometry analysis (down) of the Dao protein expression; (D) RT-qPCR analysis of the Dao expression levels in mouse lungs; (E) Representative images of HE staining of mouse lung paraffin sections (Saline control, left; BLM treated, right); (F) Representative images of Masson’s staining of mouse lung paraffin sections (Saline control, left; BLM treated, right); (G) Representative Dao and Sp-c immunofluorescence staining images of mouse lung paraffin sections showing that Dao expression was downregulated and Sp-c expression was upregulated. *p < 0.05, **p < 0.01.

Application: IF/ICC    Species: Mouse    Sample: lungs

FIGURE 1. DAO expression was downregulated in IPF lungs and BLM-induced fibrotic mouse lungs. (A) The results of IPF gene sequencing showed that DAO expression was significantly lower in IPF patients than in the controls; (B) A549 cells treated with 0.02 U/mL of BLM for 24 h. Representative immunoblot image of the whole-cell lysate of the DAO protein expression; (C) Representative immunoblot images (up) and densitometry analysis (down) of the Dao protein expression; (D) RT-qPCR analysis of the Dao expression levels in mouse lungs; (E) Representative images of HE staining of mouse lung paraffin sections (Saline control, left; BLM treated, right); (F) Representative images of Masson’s staining of mouse lung paraffin sections (Saline control, left; BLM treated, right); (G) Representative Dao and Sp-c immunofluorescence staining images of mouse lung paraffin sections showing that Dao expression was downregulated and Sp-c expression was upregulated. *p < 0.05, **p < 0.01.
2). D-serine metabolism in the medial prefrontal cortex, but not the hippocampus, is involved in AD/HD-like behaviors in SHRSP/Ezo. European Journal of Pharmacology, 2022 (PubMed: 35364072) [IF=4.2]
3). Decreased Endogenous Hydrogen Sulfide Generation in Penile Tissues of Diabetic Rats with Erectile Dysfunction. Journal of Sexual Medicine, 2016 (PubMed: 26853047) [IF=3.3]

Application: WB    Species:    Sample:

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