DAO Antibody - #DF7266

FIGURE 1. DAO expression was downregulated in IPF lungs and BLM-induced fibrotic mouse lungs. (A) The results of IPF gene sequencing showed that DAO expression was significantly lower in IPF patients than in the controls; (B) A549 cells treated with 0.02 U/mL of BLM for 24 h. Representative immunoblot image of the whole-cell lysate of the DAO protein expression; (C) Representative immunoblot images (up) and densitometry analysis (down) of the Dao protein expression; (D) RT-qPCR analysis of the Dao expression levels in mouse lungs; (E) Representative images of HE staining of mouse lung paraffin sections (Saline control, left; BLM treated, right); (F) Representative images of Masson’s staining of mouse lung paraffin sections (Saline control, left; BLM treated, right); (G) Representative Dao and Sp-c immunofluorescence staining images of mouse lung paraffin sections showing that Dao expression was downregulated and Sp-c expression was upregulated. *p < 0.05, **p < 0.01.

FIGURE 1. DAO expression was downregulated in IPF lungs and BLM-induced fibrotic mouse lungs. (A) The results of IPF gene sequencing showed that DAO expression was significantly lower in IPF patients than in the controls; (B) A549 cells treated with 0.02 U/mL of BLM for 24 h. Representative immunoblot image of the whole-cell lysate of the DAO protein expression; (C) Representative immunoblot images (up) and densitometry analysis (down) of the Dao protein expression; (D) RT-qPCR analysis of the Dao expression levels in mouse lungs; (E) Representative images of HE staining of mouse lung paraffin sections (Saline control, left; BLM treated, right); (F) Representative images of Masson’s staining of mouse lung paraffin sections (Saline control, left; BLM treated, right); (G) Representative Dao and Sp-c immunofluorescence staining images of mouse lung paraffin sections showing that Dao expression was downregulated and Sp-c expression was upregulated. *p < 0.05, **p < 0.01.

Fig. 2. Western blot analysis of SR and DAAO protein expression in the mPFC and HIP. (A–C) Protein bands of approximately 39 kDa (D-amino acid oxidase [DAAO]), 37 kDa (serine racemase [SR]), and 38 kDa (glyceraldehyde 3-phosphate dehydrogenase [GAPDH]) were detected. SR and DAAO immunorecognition signals were normalized against GAPDH one, which was used as a loading control. The right side of (A) and (B) show the results of absorption tests with DAAO peptide and SR protein. (D–G) The column graphs show densitometric analyses of DAAO and SR corresponding bands relative to the GAPDH one in the mPFC (D, E) and HIP (F, G) (WKY/Ezo, n = 12; SHRSP/Ezo, n = 8). Each value represents the mean ± S.E.M. W, WKY/Ezo; S, SHRSP/Ezo, *P < 0.05 significantly different from WKY/Ezo.