SETD2 Antibody - #DF7246

Figure 4 Circ-ITCH acts as a ceRNA to negatively regulate miR-106b-5p to target SETD2. A. miR-106b-5p expression levels were measured using qRT–PCR, which showed that miR-106b-5p expression was upregulated in PCa tissue (tumor) compared to adjacent normal tissue (normal) (n = 6, *P < 0.05, **P < 0.01, ***P < 0.001, Student's t test). B. Putative miR-106b-5p binding sequence in the 3′-UTR of circ-ITCH mRNA. C. Relative luciferase activity was determined 48 h after transfection of miR฀106b-5p mimic/NC or the wild-type (WT)/mutant (Mut) 3′UTR of circ-ITCH into DU145 cells. The data are presented as the means ± S.D.s (*P < 0.05, **P < 0.01, ***P < 0.001, Student's t test). D. The expression of miR-106b-5p in PCa cells was reduced by upregulation of circ-ITCH expression, as determined by qRT–PCR. E. Specic miR- 106b-5p binding sequence in the 3′-UTR of SETD2 mRNA. F. Construction of SETD2 luciferase reporter vectors (mutant (MUT) and wild-type (WT)). Dual luciferase reporter assays demonstrated that SETD2 is a direct target of miR-106b-5p (*P < 0.05, **P < 0.01, ***P < 0.001, Student's t test). G. qRT–PCR revealed that SETD2 expression was upregulated in PCa tissue compared to adjacent normal tissue (normal) (n = 6, *P < 0.05, **P < 0.01, ***P < 0.001, Student's t test). H. The expression of miR- 106b-5p in DU145 cells was reduced by upregulation of SETD2 expression, as determined by Western blotting. I. CircITCH upregulates SETD2 mRNA and protein expression levels in DU145 cells.