PARP1 Antibody - #DF7198

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产品: PARP1 抗体
货号: DF7198
描述: Rabbit polyclonal antibody to PARP1
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P09874
RRID: AB_2839150

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PARP1 Antibody detects endogenous levels of total PARP1.
RRID:
AB_2839150
引用格式: Affinity Biosciences Cat# DF7198, RRID:AB_2839150.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADP-ribosyltransferase diphtheria toxin-like 1; ADPRT 1; ADPRT; ADPRT1; APOPAIN; ARTD1; NAD(+) ADP-ribosyltransferase 1; PARP; PARP-1; PARP1; PARP1_HUMAN; Poly [ADP-ribose] polymerase 1; Poly ADP ribose polymerase 1; Poly[ADP-ribose] synthase 1; PPOL; SCA1;

抗原和靶标

免疫原:

A synthesized peptide derived from human PARP1, corresponding to a region within the internal amino acids.

基因/基因ID:
PARP1(142)
描述:
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Genetic Information Processing > Replication and repair > Base excision repair.

文献引用

1). Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. NUCLEIC ACIDS RESEARCH, 2020 (PubMed: 32710621) [IF=16.6]

Application: WB    Species: mouse    Sample: liver

Figure 7. Effect of A22 on ameliorating apoptosis, ER stress, inflammation, metabolic syndrome, and fibrogenesis in HF diet-fed mice. (A) Effect of A22 on BCL-2 gene transcription. (B) Effect of A22 on BAX gene transcription. (C) Effect of A22 on expressions of apoptosis-related proteins in liver. The extracted proteins from the liver were immunoblotted with specific antibodies, and quantified based on the loading control of ACTIN. (D) Effect of A22 on ER stress. The UPR proteins (IRE-1, PERK, elF-2 and CHOP) were analyzed by using western Blot. (E) Effect of A22 on expressions of inflammatory factors. (F) Effect of A22 on expressions of fibrogenic proteins.
2). Cooperative STAT3-NFkB signaling modulates mitochondrial dysfunction and metabolic profiling in hepatocellular carcinoma. Metabolism: clinical and experimental, 2024 (PubMed: 38184165) [IF=10.8]
3). Nicotinamide mononucleotide combined with PJ-34 protects microglial cells from lipopolysaccharide-induced mitochondrial impairment through NMNAT3-PARP1 axis. Journal of translational medicine, 2025 (PubMed: 40050860) [IF=7.4]
4). Danhong injection alleviates cerebral ischemia/reperfusion injury by improving intracellular energy metabolism coupling in the ischemic penumbra. Biomedicine & Pharmacotherapy, 2021 (PubMed: 34058441) [IF=6.9]

Application: WB    Species: rat    Sample:

Fig. 4.| Effect of DHI on the activity of PARP1/AIF signaling pathway and the content of molecules associated with cytoplasmic glycolysis. A-E: Representative images of WB analysis and the semi-quantification of PARP1, PAR, AIF, and HSP70. Data are expressed as mean ± SD (n = 3).

Application: IHC    Species: rat    Sample: brain

Fig. 5.| Effect of DHI on the content of molecules associated with cytoplasmic glycolysis. A-F: Immunostaining photomicrographs of PARP1, AIF, HSP70 and quantitative analysis of the IOD.
5). Perilla frutescens L. alleviates trimethylamine N-oxide–induced apoptosis in the renal tubule by regulating ASK1-JNK phosphorylation. Phytotherapy Research, 2023 (PubMed: 36420586) [IF=6.1]
6). Exploring the Critical Components and Therapeutic Mechanisms of Perilla frutescens L. in the Treatment of Chronic Kidney Disease via Network Pharmacology. Frontiers in Pharmacology, 2021 (PubMed: 34899287) [IF=5.6]

Application: WB    Species: Rat    Sample: NRK-52E cells

FIGURE 8 Luteolin reduces ADR-induced apoptosis. (A) Effects of luteolin in ADR-induced apoptosis by TUNEL and DAPI staining assay. NRK-52E cells were incubated with ADR for 24 h in the presence and absence of luteolin. Apoptotic cells were detected by TUNEL and DAPI staining. Fluorescence staining was observed by fluorescence microscope (magnification ×200) and the analysis result on the right was expressed as the percentages of dead cells compared with the Ctrl (mean ± SD, n = 3; *p
7). Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway. Marine Drugs, 2018 (PubMed: 29509717) [IF=5.4]

Application: WB    Species: mouse    Sample:

Figure 5. | Effects on apoptosis-related protein expression, poly ADP ribose polymerase (PARP), and caspase-3 activity in vivo. After pretreated with MPTP for seven days, the C57BL/6 mice were administrated with MA or different concentrations of OP for the followed 7 days. (A): Original bands of cytochrome c (CytC), cleaved caspased-3, B-cell lymphoma 2 (Bcl-2), BCL2 associated X (Bax), PARP, and β-actin.
8). FGFRL1 affects chemoresistance of small-cell lung cancer by modulating the PI3K/Akt pathway via ENO1. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020 (PubMed: 31957179) [IF=5.3]

Application: WB    Species: Human    Sample: SCLC cells

Figure 3 FGFRL1 induces chemoresistance of SCLC mainly by decreasing drug‐induced apoptosis and cell cycle arrest. A, B, Cell apoptosis and cell cycle arrest were evaluated by flow cytometric analysis in FGFRL1–down‐regulated SCLC cells after ADM exposure. C, D, Flow cytometric analysis of cell apoptosis and cell cycle arrest induced by ADM in FGFRL1‐overexpressing SCLC cells. E, F, Apoptosis‐related proteins were measured by Western blot following anticancer drug exposure in SCLC cells with down‐regulated or up‐regulated FGFRL1 expression. **P < .01; ***P < .001
9). Zinc defends against Parthanatos and promotes functional recovery after spinal cord injury through SIRT3-mediated anti-oxidative stress and mitophagy. CNS neuroscience & therapeutics, 2023 (PubMed: 37063066) [IF=4.8]

Application: IF/ICC    Species: Mouse    Sample:

FIGURE 1. Zinc can inhibit Parthanatos in the spinal cord of SCI mice. (A) Western blot and (B) statistical results of western blots of PARP‐1 (n = 5, one‐way ANOVA followed by Bonferroni's post hoc test). (C) Immunofluorescence and (D) statistical analysis of immunofluorescence staining of PARP‐1 in nerve cells from each group (Scale bar = 50 μm, n = 3, one‐way ANOVA followed by Games–Howell post hoc test). (E) Mitochondrial membrane potential was measured with the JC‐1 probe (n = 5, one‐way ANOVA followed by Bonferroni's post hoc test). (F, H, J) Western blot and (G, I, K) statistical results of western blots of Mito‐AIF, Cyto‐AIF, and Nucleo‐AIF (n = 5, one‐way ANOVA followed by Bonferroni's post hoc test). The data represent means ± SD, * means, p 

Application: WB    Species: Mouse    Sample:

FIGURE 1. Zinc can inhibit Parthanatos in the spinal cord of SCI mice. (A) Western blot and (B) statistical results of western blots of PARP‐1 (n = 5, one‐way ANOVA followed by Bonferroni's post hoc test). (C) Immunofluorescence and (D) statistical analysis of immunofluorescence staining of PARP‐1 in nerve cells from each group (Scale bar = 50 μm, n = 3, one‐way ANOVA followed by Games–Howell post hoc test). (E) Mitochondrial membrane potential was measured with the JC‐1 probe (n = 5, one‐way ANOVA followed by Bonferroni's post hoc test). (F, H, J) Western blot and (G, I, K) statistical results of western blots of Mito‐AIF, Cyto‐AIF, and Nucleo‐AIF (n = 5, one‐way ANOVA followed by Bonferroni's post hoc test). The data represent means ± SD, * means, p 
10). Perillaldehyde targeting PARP1 to inhibit TRPM2-CaMKII/CaN signal transduction in diabetic cardiomyopathy. International immunopharmacology, 2025 (PubMed: 39970708) [IF=4.8]
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