Connexin 43 / GJA1 Antibody - #AF0137

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产品: Connexin 43 / GJA1 抗体
货号: AF0137
描述: Rabbit polyclonal antibody to Connexin 43 / GJA1
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P17302
RRID: AB_2833319

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Connexin 43 / GJA1 Antibody detects endogenous levels of total Connexin 43 / GJA1.
RRID:
AB_2833319
引用格式: Affinity Biosciences Cat# AF0137, RRID:AB_2833319.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Connexin 43; Connexin-43; Cx 43; Cx43; CXA1_HUMAN; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; Gap junction protein alpha 1 43kDa (connexin 43); Gap junction protein alpha 1 43kDa; Gap junction protein alpha like; GJA 1; Gja1; GJAL; ODD; ODDD; ODOD; SDTY3;

抗原和靶标

免疫原:

A synthesized peptide derived from human Connexin 43 / GJA1, corresponding to a region within the internal amino acids.

基因/基因ID:
GJA1(2697)
描述:
Cx43 an integral membrane protein of the connexin family, alpha-type (group II) subfamily. Hexamers of connexin-43 form connexons, which aggregate together to form gap junctions, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

文献引用

1). Protective effect of oxyberberine against acute lung injury in mice via inhibiting RhoA/ROCK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 35753262) [IF=6.9]
2). USP38 exacerbates pressure overload-induced left ventricular electrical remodeling. Molecular medicine (Cambridge, Mass.), 2024 (PubMed: 38937697) [IF=6.0]

Application: WB    Species: Mouse    Sample:

Fig. 5 USP38 reduces the expression of Cx43 in mice four weeks after the AB surgery. A The left ventricle of mice was stained with anti-Cx43 antibody (red) and DAPI (blue). Representative Cx43 immunofluorescence staining images in cardiac-conditional USP38 knockout mice (n = 4). B, C Representative Western blotting and statistical analysis of Cx43 in cardiac-conditional USP38 knockout mice (n = 3). * P 

Application: IF/ICC    Species: Mouse    Sample:

Fig. 5 USP38 reduces the expression of Cx43 in mice four weeks after the AB surgery. A The left ventricle of mice was stained with anti-Cx43 antibody (red) and DAPI (blue). Representative Cx43 immunofluorescence staining images in cardiac-conditional USP38 knockout mice (n = 4). B, C Representative Western blotting and statistical analysis of Cx43 in cardiac-conditional USP38 knockout mice (n = 3). * P 
3). Lithospermic acid alleviates oxidative stress and inflammation in DSS-induced colitis through Nrf2. European journal of pharmacology, 2025 (PubMed: 39956261) [IF=4.2]
4). Carbenoxolone has the potential to ameliorate acute incision pain in rats. Molecular Medicine Reports, 2021 (PubMed: 34013377) [IF=3.4]

Application: IF/ICC    Species: rat    Sample:

Figure 4.| Co‑localization of Cx43 and GFAP in IP model rats. Immunofluorescence assay was used to evaluate the co‑localization of Cx43 (green) and GFAP(red). Magnification, x200; scale bar, 10 µm. C, control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin‑1 mimetic inhibitory peptide; Cx43,connexin 43; GFAP, glial fibrillary acidic protein.
5). GJA1 reverses arsenic-induced EMT via modulating MAPK/ERK signaling pathway. TOXICOLOGY AND APPLIED PHARMACOLOGY, 2022 (PubMed: 35750204) [IF=3.3]
6). AKAP95 regulates ubiquitination and degradation of cyclin Ds/Es, influencing the G1/S transition of lung cancer cells. Molecular carcinogenesis, 2024 (PubMed: 38923703) [IF=3.0]
7). Dapagliflozin Improves Diabetic Cardiomyopathy by Modulating the Akt/MTOR Signaling Pathway. Biomed Research International, 2022 (PubMed: 35928916) [IF=2.6]

Application: WB    Species: Rat    Sample:

Figure 3 Immunohistochemical staining for Cx43 (×400): A: normal control group, B: normal administration group, C: diabetes control group, D: early administration group, and E: late administration group. ∗p < 0.05 vs. A; &p > 0.05vs. A; #p < 0.05vs. C; @p < 0.05vs. D.
8). Connexin 43 overexpression induces lung cancer angiogenesis in vitro following phosphorylation at Ser279 in its C‑terminus. Oncology Letters, 2022 (PubMed: 35949588) [IF=2.5]

Application: WB    Species: Human    Sample: HPMECs

Figure 2. Relative expression levels (normalized with NC) of various proteins in different groups as determined through western blotting (the resulting data are presented as the mean ± standard deviation; *P
9). Correlations of the expression of Cx43, SCFFBXW7, p-cyclin E1 (Ser73), p-cyclin E1 (Thr77) and p-cyclin E1 (Thr395) in colon cancer tissues. World journal of gastrointestinal oncology, 2025 (PubMed: 39817129) [IF=2.5]
10). Effect of high-throughput screening of circRNA01724 on a rat model of myocardial ischemia ventricular arrhythmia. Journal of thoracic disease, 2022 (PubMed: 35572876) [IF=2.1]
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