产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0137, RRID:AB_2833319.
展开/折叠
Connexin 43; Connexin-43; Cx 43; Cx43; CXA1_HUMAN; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; Gap junction protein alpha 1 43kDa (connexin 43); Gap junction protein alpha 1 43kDa; Gap junction protein alpha like; GJA 1; Gja1; GJAL; ODD; ODDD; ODOD; SDTY3;
抗原和靶标
- P17302 CXA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P17302 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K9 | Ubiquitination | Uniprot | |
K109 | Ubiquitination | Uniprot | |
K128 | Ubiquitination | Uniprot | |
K136 | Ubiquitination | Uniprot | |
Y137 | Phosphorylation | Uniprot | |
K144 | Ubiquitination | Uniprot | |
R148 | Methylation | Uniprot | |
K243 | Ubiquitination | Uniprot | |
S244 | Phosphorylation | Uniprot | |
Y247 | Phosphorylation | P12931 (SRC) | Uniprot |
T250 | Phosphorylation | Uniprot | |
S251 | Phosphorylation | Uniprot | |
S255 | Phosphorylation | P28482 (MAPK1) , P06493 (CDK1) , P27361 (MAPK3) , Q13164 (MAPK7) | Uniprot |
K258 | Ubiquitination | Uniprot | |
S262 | Phosphorylation | P17252 (PRKCA) | Uniprot |
K264 | Ubiquitination | Uniprot | |
Y265 | Phosphorylation | P12931 (SRC) | Uniprot |
Y267 | Phosphorylation | Uniprot | |
S272 | Phosphorylation | Uniprot | |
T275 | Phosphorylation | Uniprot | |
S279 | Phosphorylation | Q16539 (MAPK14) , P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
S282 | Phosphorylation | P28482 (MAPK1) , Q16539 (MAPK14) , P27361 (MAPK3) , Q13164 (MAPK7) | Uniprot |
Y286 | Phosphorylation | Uniprot | |
K287 | Ubiquitination | Uniprot | |
T290 | Phosphorylation | Uniprot | |
S296 | Phosphorylation | Uniprot | |
S297 | Phosphorylation | Uniprot | |
Y301 | Phosphorylation | Uniprot | |
K303 | Ubiquitination | Uniprot | |
S306 | Phosphorylation | Uniprot | |
Y313 | Phosphorylation | Uniprot | |
S314 | Phosphorylation | Uniprot | |
S325 | Phosphorylation | P48730 (CSNK1D) | Uniprot |
T326 | Phosphorylation | Uniprot | |
S328 | Phosphorylation | P48730 (CSNK1D) | Uniprot |
S330 | Phosphorylation | P48730 (CSNK1D) | Uniprot |
S344 | Phosphorylation | Uniprot | |
K345 | Ubiquitination | Uniprot | |
K346 | Ubiquitination | Uniprot | |
S364 | Phosphorylation | Uniprot | |
S365 | Phosphorylation | Q02156 (PRKCE) , P17612 (PRKACA) | Uniprot |
S368 | Phosphorylation | P17252 (PRKCA) , P17612 (PRKACA) , Q02156 (PRKCE) , P05771 (PRKCB) , P05129 (PRKCG) , Q05655 (PRKCD) | Uniprot |
S369 | Phosphorylation | P31749 (AKT1) , Q02156 (PRKCE) , P17612 (PRKACA) | Uniprot |
S372 | Phosphorylation | Uniprot | |
S373 | Phosphorylation | P17612 (PRKACA) , P31749 (AKT1) , Q02156 (PRKCE) | Uniprot |
研究背景
Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).
Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (By similarity).
Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2.
S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.
Acetylated in the developing cortex; leading to delocalization from the cell membrane.
Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction. Endoplasmic reticulum.
Note: Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65.
Expressed in the heart and fetal cochlea.
A connexon is composed of a hexamer of connexins. Interacts (via C-terminus) with TJP1 (By similarity). Interacts (via C-terminus) with SRC (via SH3 domain) (By similarity). Interacts (not ubiquitinated) with UBQLN4 (via UBA domain) (By similarity). Interacts with SGSM3 and CNST (By similarity). Interacts with RIC1/CIP150. Interacts with CSNK1D. Interacts with NOV. Interacts with TMEM65 (By similarity).
Belongs to the connexin family. Alpha-type (group II) subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
文献引用
Application: WB Species: Mouse Sample:
Application: IF/ICC Species: Mouse Sample:
Application: IF/ICC Species: rat Sample:
Application: WB Species: Human Sample: HPMECs
Application: WB Species: rat Sample: sacral spinal cord
Application: WB Species: Human Sample: A549 cells
Application: WB Species: Human Sample: A549 and Beas-2B cells
Application: WB Species: Rat Sample:
限制条款
产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.