产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7175, RRID:AB_2839127.
展开/折叠
DNA repair protein RAD52; DNA repair protein RAD52; DNA repair protein RAD52 homolog; RAD 52; Rad52; RAD52 homolog (S. cerevisiae); RAD52 homolog (S. cerevisiae); RAD52 homolog; RAD52_HUMAN; Recombination protein RAD52; Recombination protein RAD52; Rhabdomyosarcoma antigen MU RMS 40.23;
抗原和靶标
- P43351 RAD52_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKALRQRLGPEYISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHEDVGYGVSEGLKSKALSLEKARKEAVTDGLKRALRSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQDLEPSVEEARYNSCRPNMALGHPQLQQVTSPSRPSHAVIPADQDCSSRSLSSSAVESEATHQRKLRQKQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPPVTHSTPVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWAVTPDAGDGVVKPSSRADPAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQRTTGNWESHRKSQDMKKRKYDPS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P43351 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| Y104 | Phosphorylation | P00519 (ABL1) , A0A173G4P4 (Abl fusion) , A9UF07 (BCR/ABL fusion) | Uniprot |
| S129 | Phosphorylation | Uniprot | |
| K133 | Acetylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| Y171 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| K177 | Acetylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K190 | Acetylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| Y205 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| T223 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| K262 | Acetylation | Uniprot | |
| K274 | Acetylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| K286 | Acetylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| K323 | Acetylation | Uniprot | |
| T335 | Phosphorylation | Uniprot | |
| K344 | Acetylation | Uniprot | |
| T397 | Phosphorylation | Uniprot | |
| K406 | Acetylation | Uniprot | |
| K411 | Sumoylation | Uniprot | |
| K412 | Acetylation | Uniprot | |
| K412 | Sumoylation | Uniprot | |
| K414 | Acetylation | Uniprot | |
| K414 | Sumoylation | Uniprot |
研究背景
Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR.
Nucleus.
The full-length protein forms heptameric rings. Interacts with ABL1. Interacts with RPA2; the interaction is direct and associates RAD52 with the RPA complex.
Belongs to the RAD52 family.
研究领域
· Genetic Information Processing > Replication and repair > Homologous recombination.
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