PDX1 Antibody - #DF7170

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(4)   (6)  
产品: PDX1 抗体
货号: DF7170
描述: Rabbit polyclonal antibody to PDX1
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P52945
RRID: AB_2839122

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PDX1 Antibody detects endogenous levels of total PDX1.
RRID:
AB_2839122
引用格式: Affinity Biosciences Cat# DF7170, RRID:AB_2839122.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Glucose sensitive factor; Glucose-sensitive factor; GSF; IDX 1; IDX-1; IDX1; Insulin promoter factor 1; Insulin promoter factor 1 homeodomain transcription factor; Insulin upstream factor 1; IPF 1; IPF-1; IPF1; Islet/duodenum homeobox 1; Islet/duodenum homeobox-1; IUF 1; IUF-1; IUF1; MODY4; Pancreas/duodenum homeobox 1; Pancreas/duodenum homeobox protein 1; pancreatic and duodenal homeobox P; PDX 1; PDX-1; PDX1; PDX1_HUMAN; Somatostatin transactivating factor 1; Somatostatin-transactivating factor 1; STF 1; STF-1; STF1;

抗原和靶标

免疫原:

A synthesized peptide derived from human PDX1, corresponding to a region within the internal amino acids.

基因/基因ID:
PDX1(3651)
描述:
The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).

研究领域

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

文献引用

1). Wheat starch-Lonicera caerulea berry polyphenols complex regulates blood glucose and improves intestinal flora in type 2 diabetic mice. Carbohydrate polymers, 2025 (PubMed: 39778992) [IF=10.7]
2). Sall2 knockdown exacerbates palmitic acid induced dysfunction and apoptosis of pancreatic NIT-1 beta cells. BIOMEDICINE & PHARMACOTHERAPY, 2018 (PubMed: 29783189) [IF=6.9]

Application: WB    Species: mouse    Sample: NIT-1-shc and NIT-1-sh2 cells

Fig. 4.| Sall2 knockdown impaired insulin synthesis and secretion in NIT-1 cell. NIT-1-sh2 cells and NIT-1-shc cells were incubated with or without 0.5 mM PA for 48 h. (A) GSIS was performed in NIT-1-shc cells and NIT-1-sh2 cells. (B) Pdx-1, MafA, Ins1, and Ins2 mRNA levels in NIT-1-shc and NIT-1-sh2 cells. (C)Western blot analysis of Sall2 and Pdx-1 expression levels in NIT-1-shc and NIT-1-sh2 cells. Data represent mean ± SD of three independent experiments. *p < 0.05,**p < 0.01, ***p < 0.001.
3). A New Way for Beta Cell Neogenesis: Transdifferentiation from Alpha Cells Induced by Glucagon-Like Peptide 1. Journal of Diabetes Research, 2019 (PubMed: 31001561) [IF=3.6]

Application: WB    Species: rat    Sample: islets

Figure 5: | Western blotting analysis for Akt, FoxO1, Pdx1, MafA, and MafB in rat islets. GAPDH was used as a loading control (#P < 0 05 vs.NC; ∗P < 0 05 vs. DM; •P < 0 05 vs. GLP1 50 μg/kg; and ΔP < 0 05 vs. GLP1 100 μg/kg). GLP1, glucagon-like peptide 1; NC, negative control;DM, diabetes mellitus; Akt, AKT kinase; FoxO1, forkhead box O 1; Pdx1, pancreatic and duodenal homeobox 1; MafA, MAF BZIP transcription factor A; MafB, MAF BZIP transcription factor B; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
4). Nickel Sulfate-Induced GSIS Injury in MIN6 Cells by Activating the JNK Pathway Through Oxidative Stress. Biological trace element research, 2024 (PubMed: 39661292) [IF=3.4]

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.