POMC Antibody - #DF7154

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产品: POMC 抗体
货号: DF7154
描述: Rabbit polyclonal antibody to POMC
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Dog, Chicken
分子量: 29kDa; 29kD(Calculated).
蛋白号: P01189
RRID: AB_2839106

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(80%), Bovine(90%), Sheep(90%), Dog(90%), Chicken(80%)
克隆:
Polyclonal
特异性:
POMC Antibody detects endogenous levels of total POMC.
RRID:
AB_2839106
引用格式: Affinity Biosciences Cat# DF7154, RRID:AB_2839106.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACTH; Adrenocorticotropic hormone; Adrenocorticotropin; Alpha melanocyte stimulating hormone; alpha-MSH; alphaMSH; Beta LPH; Beta melanocyte stimulating hormone; Beta-endorphin; beta-MSH; CLIP; Corticotropin; Corticotropin lipotropin; Corticotropin-like intermediary peptide; Gamma LPH; gamma-MSH; Lipotropin beta; Lipotropin gamma; Lipotropin, included; LPH; Melanocyte-stimulating hormone, included; Melanotropin alpha; Melanotropin beta; Melanotropin gamma; Melanotropin, included; Met-enkephalin; MSH; NPP; Opiomelanocortin prepropeptide; POC; POMC; Pomc-1; Pomc1; Pomc2; Pro ACTH endorphin; Pro opiomelanocortin; Pro-opiomelanocortin-alpha; Proopiomelanocortin; Proopiomelanocortin preproprotein; Tetracosactide;

抗原和靶标

免疫原:
Uniprot:

P01189(COLI_HUMAN) >>Visit HPA database.

基因/基因ID:
POMC(5443)
表达:
P01189 COLI_HUMAN:

ACTH and MSH are produced by the pituitary gland.

描述:
This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation.
序列:
MPRSCCSRSGALLLALLLQASMEVRGWCLESSQCQDLTTESNLLECIRACKPDLSAETPMFPGNGDEQPLTENPRKYVMGHFRWDRFGRRNSSSSGSSGAGQKREDVSAGEDCGPLPEGGPEPRSDGAKPGPREGKRSYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEFKRELTGQRLREGDGPDGPADDGAGAQADLEHSLLVAAEKKDEGPYRMEHFRWGSPPKDKRYGGFMTSEKSQTPLVTLFKNAIIKNAYKKGE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
90
Sheep
90
Dog
90
Pig
80
Chicken
80
Horse
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P01189 作为底物

Site PTM Type Enzyme
T71 O-Glycosylation
S92 Phosphorylation
S95 Phosphorylation
S97 Phosphorylation
S108 Phosphorylation
S168 Phosphorylation
S230 Phosphorylation

研究背景

功能:

Stimulates the adrenal glands to release cortisol.

Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes.

Increases the pigmentation of skin by increasing melanin production in melanocytes.

Endogenous orexigenic opiate.

Endogenous opiate.

翻译修饰:

Specific enzymatic cleavages at paired basic residues yield the different active peptides.

O-glycosylated; reducing sugar is probably N-acetylgalactosamine.

细胞定位:

Secreted.
Note: Melanocyte-stimulating hormone alpha and beta-endorphin are stored in separate granules in hypothalamic POMC neurons, suggesting that secretion may be under the control of different regulatory mechanisms.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

ACTH and MSH are produced by the pituitary gland.

蛋白家族:

Belongs to the POMC family.

研究领域

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

文献引用

1). Metabolic regulation of Ganoderma lucidum extracts in high sugar and fat diet-induced obese mice by regulating the gut-brain axis. Journal of Functional Foods [IF=5.6]

Application: WB    Species: Mouse    Sample: hypothalamus

Fig. 12. AGL regulates the leptin-mediated related signaling molecules. The expression of LEPR, AGPR, NPY, POMC and GAD65 in the hypothalamus assessed by western blotting. Data are presented as the means ± SD of more than 3 independent experiments. *p 
2). Chronic heat stress alters hypothalamus integrity, the serum indexes and attenuates expressions of hypothalamic appetite genes in broilers. Journal of Thermal Biology (PubMed: 30975407) [IF=2.7]

Application: IF/ICC    Species: broilers    Sample: hypothalamus

Fig. 6.| Effect of chronic heat stress on protein levels of POMC in the hypothalamus of broilers after 14 days heat stress based on the whole-brain immunofluorescent method of the control group (A), heat stress group (B), pair-fed group (C). POMC, proopiomelanocortin; White arrows on the right panels indicates POMC proteins which colors are red. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).

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