LHB Antibody - #DF7140

Figure 2. LncSM2 knockdown inhibited the secretion of gonadotropins in pituitary cells. (A) Morphology of pituitary cells under a microscope. The left picture shows F1 generation pituitary cells, and the right picture shows F2 generation pituitary cells. (B) Identification of sheep pituitary cells by FSHβ and LHβ marker gene immunofluorescence staining. Scale bar, 100 μm. (C) mRNA expression of lncRNA SM2 in sheep pituitary cells treated with si-lncRNA SM2-2592, si-lncRNA SM2-3415 and si-lncRNA SM2-3794 was determined by qPCR. (D) FSH and LH secretion was detected by ELISA. (E,F) mRNA and protein expression of FSHβ and LHβ in pituitary cells in each group. All data are shown as the mean ± SEM of at least three replicates (* p < 0.05; ** p < 0.01).

Figure 2. LncSM2 knockdown inhibited the secretion of gonadotropins in pituitary cells. (A) Morphology of pituitary cells under a microscope. The left picture shows F1 generation pituitary cells, and the right picture shows F2 generation pituitary cells. (B) Identification of sheep pituitary cells by FSHβ and LHβ marker gene immunofluorescence staining. Scale bar, 100 μm. (C) mRNA expression of lncRNA SM2 in sheep pituitary cells treated with si-lncRNA SM2-2592, si-lncRNA SM2-3415 and si-lncRNA SM2-3794 was determined by qPCR. (D) FSH and LH secretion was detected by ELISA. (E,F) mRNA and protein expression of FSHβ and LHβ in pituitary cells in each group. All data are shown as the mean ± SEM of at least three replicates (* p < 0.05; ** p < 0.01).

Figure 7. Spp1 promotes FSH synthesis and secretion. (A) Pituitary single-cell transcriptomic analysis of Spp1 mRNA enrichment in different cell populations. (B) Immunofluorescence analysis OPN (green), FSHB (red), and LHB (purple) in pituitary tissue of 4-week-old and 12-week-old rats. The white arrows point to representative co-localization. (C) RT-qPCR analysis Spp1 mRNA expression in LβT2 cells transfected with siNC and three Spp1 siRNAs (n = 3). (D) RT-qPCR analysis Fshb mRNA expression transfected with siNC and Spp1 siRNA in LβT2 cells (n = 3). (E) ELISA analysis secretion level of FSH transfected with siNC and Spp1 siRNA in LβT2 cells (n = 9). (F) RT-qPCR analysis Spp1 mRNA expression in LβT2 cells transfected with vector and different quality of Spp1 plasmid (n = 3). (G) RT-qPCR analysis Fshb mRNA expression transfected with vector and Spp1 plasmid (1000 ng) in LβT2 cells (n = 3). (H) ELISA analysis secretion level of FSH transfected with vector and Spp1 plasmid (1000 ng) in LβT2 cells (n = 9). **, p < 0.01; ***, p < 0.001. Cort, corticotropes; End, endothelial; Epi, epithelial; Gon, gonadotrope; Imm, immune cells; Lac, lactotropes; Mel, melanotropes; Mes, mesenchymal; Mki67+, Mki67+ proliferating cells; Per, pericytes; PL, posterior lobe; Som, somatotropes; SomLac, somatolactotropes; Fsc, folliculostellate cells; Others, other cells.