Leptin Receptor Antibody - #DF7139

Figure 4. Leptin signaling pathway (MAPK/ERK) was activated in the knee cartilage of 7-day male mice from the maternal obesity group. (A) Plasma leptin level and (B) Lepr mRNA level of knee cartilage 7-day male mice offspring in control versus obesity groups. (C) Correlation between Lepr and cartilage key indicators mRNA expression levels. (D) Representative western blot bands of the expression of leptin receptor in the cell cytoplasm and phosphorylated ERK (p-ERK), phosphorylated p38 (p-38), and phosphorylated c-Jun N-terminal kinase (p-JNK) in the nucleus from knee cartilage of the offspring. (E) Statistical results of the expression of key proteins of MAPK signaling pathway in knee cartilage of the offspring. (A) n = 7, (B) n = 6, (C) n = 6, and (D) n = 6; each band represents the expression of cartilage tissue proteins in two mice. All mice in each group from at least three litters of different mothers. O-WT-P7: offspring 7-day male mice from the control mothers. O-OB-P7: offspring 7-day male mice from obese mothers. Results are expressed as mean ± SD. ns not significant, * p < 0.05, ** p < 0.01, and *** p < 0.001.

Figure 5 Effects of the water extract of fermented rice bran (FRB) on hepatic lipid metabolism-related protein expressions in aged rats with high-fat (HF) diet feeding. Values are presented as the mean ± standard deviation (n = 6). * p < 0.05 vs. the normal control (NC) group; significance between two groups was determined using Student’s t-test. In the HF diet-fed groups, significant differences between groups were determined by a one-way ANOVA with Fisher‘s post hoc test. Western blot analysis of (A) NAD-dependent sirtuin-1 (SIRT1), (B) adenosine monophosphate-activated protein kinase-α (AMPKα), (C) phosphorylated (p)-AMPKα, (D) p-AMPKα/AMPKα, (E) leptin receptor, and (F) adiponectin receptor 2 (AdipoR2) protein expressions. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) was used as an internal control. Quantitative analysis of protein levels and the ratio of each internal control were calculated by setting the value of the mean of the NC group.

Figure 3. The relative mRNA (A) and protein (B) levels of OB-RGRP in the cells transfected with OB-RGRP siRNA and expression vector which were determined by RT-PCR and western blot, respectively. si-OB-RGRP1: OB-RGRP-siRNA-1; si-OB-RGRP2: OB-RGRP-siRNA-2; si-OB-RGRP3: OB-RGRP-siRNA-3; si-OB-RGRP NC: OB-RGRP-siRNA negative control; Vector: empty expression vector; OB-RGRP: OB-RGRP expression vector. *p 

Figure 4 Quantitative analysis of the intensity of leptin IR in (A) villi and (B) crypts of small intestine segments, measured by comparison of the optical density (OD) of the microscopic images converted to 8-bit, grey-scale images. (C) Representative images of the leptin IR in sections of duodenum. Quantitative analysis of the intensity of leptin receptor IR in (D) villi and (E) crypts of small intestine segments, measured by comparison of the optical density (OD) of the microscopic images converted to 8-bit, grey-scale images. (F) Representative images of the ghrelin IR in sections of proximal jejunum. (C), (F): All scale bars represent 100 µm. (A), (B), (D), (E): Bar plots show lsmeans value and standard deviation (whiskers). A p-value range was attributed above plots when two groups exhibit significant differences: *p value 

Fig. 12. AGL regulates the leptin-mediated related signaling molecules. The expression of LEPR, AGPR, NPY, POMC and GAD65 in the hypothalamus assessed by western blotting. Data are presented as the means ± SD of more than 3 independent experiments. *p 

Fig. 4. Leptin promoted the AKT-mTOR signaling activities. (a) Western blot analysis result of (a) TSPCs treated with different concentrations of leptin (0, 10, 50, 100 and 200 ng/mL) for 48 h; (b) a time course of LC3-I/II protein expression in TSPCs treated with 100 ng/mL leptin. Results were representatives of at least 3 biological replicates.