产品: Collagen II 抗体
货号: AF0135
描述: Rabbit polyclonal antibody to Collagen II
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 140kDa, 30~50kd(possible isoform); 142kD(Calculated).
蛋白号: P02458
RRID: AB_2833318

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(86%), Xenopus(86%)
克隆:
Polyclonal
特异性:
Collagen II Antibody detects endogenous levels of total Collagen II.
RRID:
AB_2833318
引用格式: Affinity Biosciences Cat# AF0135, RRID:AB_2833318.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alpha 1 type II collagen;Alpha-1 type II collagen;AOM;Cartilage collagen;Chondrocalcin;CO2A1_HUMAN;COL11A3;Col2a1;Collagen II alpha 1 polypeptide;SEDC;col 2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P02458 CO2A1_HUMAN:

Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.

描述:
COL2A1 Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Belongs to the fibrillar collagen family. Homotrimers of alpha 1(II) chains. 3 isoforms of the human protein are produced by alternative splicing
序列:
MIRLGAPQTLVLLTLLVAAVLRCQGQDVQEAGSCVQDGQRYNDKDVWKPEPCRICVCDTGTVLCDDIICEDVKDCLSPEIPFGECCPICPTDLATASGQPGPKGQKGEPGDIKDIVGPKGPPGPQGPAGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPPGPPGPPGPPGLGGNFAAQMAGGFDEKAGGAQLGVMQGPMGPMGPRGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPPGKPGDDGEAGKPGKAGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDGQPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGPRGEPGTPGSPGPAGASGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIAGFKGEQGPKGEPGPAGPQGAPGPAGEEGKRGARGEPGGVGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLAGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPGLRGLPGKDGETGAAGPPGPAGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKPGDQGVPGEAGAPGLVGPRGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGASGPAGPPGAQGPPGLQGMPGERGAAGIAGPKGDRGDVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPAGSAGARGAPGERGETGPPGPAGFAGPPGADGQPGAKGEQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGSNGNPGPPGPPGPSGKDGPKGARGDSGPPGRAGEPGLQGPAGPPGEKGEPGDDGPSGAEGPPGPQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGRDGAAGVKGDRGETGAVGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIQGPQGPRGDKGEAGEPGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGANGIPGPIGPPGPRGRSGETGPAGPPGNPGPPGPPGPPGPGIDMSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPANVPKKNWWSSKSKEKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGPVCFL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Xenopus
86
Chicken
86
Dog
67
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P02458 作为底物

Site PTM Type Enzyme
Y41 Phosphorylation
K490 Ubiquitination
S536 Phosphorylation
K542 Acetylation
K764 Acetylation
K773 Acetylation
T1307 Phosphorylation
S1416 Phosphorylation

研究背景

功能:

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

翻译修饰:

The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2.

Contains mostly 4-hydroxyproline. Prolines at the third position of the tripeptide repeating unit (G-X-P) are 4-hydroxylated in some or all of the chains.

Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline.

Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains.

O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.

亚基结构:

Homotrimers of alpha 1(II) chains.

蛋白家族:

The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function (By similarity).

Belongs to the fibrillar collagen family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Digestive system > Protein digestion and absorption.

文献引用

1). Hydrogen Ion Capturing Hydrogel Microspheres for Reversing Inflammaging. Advanced materials (Deerfield Beach, Fla.) (PubMed: 37699155) [IF=29.4]

2). HSP70 attenuates compression-induced apoptosis of nucleus pulposus cells by suppressing mitochondrial fission via upregulating the expression of SIRT3. EXPERIMENTAL AND MOLECULAR MEDICINE (PubMed: 35338257) [IF=12.8]

3). Opsonized nanoparticles target and regulate macrophage polarization for osteoarthritis therapy: A trapping strategy. Journal of Controlled Release (PubMed: 35489544) [IF=10.8]

4). Co-culture pellet of human Wharton’s jelly mesenchymal stem cells and rat costal chondrocytes as a candidate for articular cartilage regeneration: in vitro and in vivo study. Stem Cell Research & Therapy (PubMed: 35907866) [IF=7.5]

Application: IHC    Species: Rat    Sample:

Fig. 3Histological and immunochemistry staining of pellets with semiquantitative analysis. A H–E and Safranin-O staining of pellets and partial enlargement in five groups. B Immunochemistry staining of COLII and COLX of pellets and partial enlargement in five groups. C–E Semiquantitative analysis of AOI of each staining (n = 4). Significant difference symbols: *p < 0.05, **p < 0.01 compared to CCs group, #p < 0.05, ##p < 0.01 compared to hWJMSCs group

5). MicroRNA-145 overexpression attenuates apoptosis and increases matrix synthesis in nucleus pulposus cells. LIFE SCIENCES (PubMed: 30797016) [IF=6.1]

Application: WB    Species: rat    Sample: NP cells

Fig. 3. |miR-145 increased matrix metabolism in rat NP cells.(A, B) Real-time PCR analysis was performed to evaluate the mRNA expression of aggrecan (A) and collagen IIa (B) after a single transfection with agomir-145 or miR-155 inhibitors. (C, D, E) Western blotting (C) and subsequent densitometric analysis (D) demonstrated that aggrecan (C, E) and collagen IIa (D, E) protein were significantly upregulated in rat NP cells after transfection with agomir-145.

6). Lysyl oxidase inhibits TNF-α induced rat nucleus pulposus cell apoptosis via regulating Fas/FasL pathway and the p53 pathways. LIFE SCIENCES (PubMed: 32979358) [IF=6.1]

Application: WB    Species: rat    Sample: NP cells

Fig. 3. |LOX improved the ECM expression pattern in TNF-α-treated rat NP cells. A. and B. Relative mRNA expressions of type II collagen and aggrecan in rat NP cells with different treatments were analyzed by RT-qPCR. C. Protein expressions of type II collagen and aggrecan in rat NP cells with different treatments were analyzed by western blot.

7). L-Glutamine alleviates osteoarthritis by regulating lncRNA-NKILA expression through the TGF-β1/SMAD2/3 signalling pathway. Clinical Science (PubMed: 35730575) [IF=6.0]

8). PD184352 exerts anti-inflammatory and antioxidant effects by promoting activation of the Nrf2/HO-1 axis. Biochemical Pharmacology (PubMed: 37028460) [IF=5.8]

9). Nerve growth factor promotes ASIC1a expression via the NF-κB pathway and enhances acid-induced chondrocyte apoptosis. International Immunopharmacology (PubMed: 32146316) [IF=5.6]

Application: IHC    Species: rat    Sample: articular chondrocytes

Fig. 1. |NGF up-regulated ASIC1a expression in rat articular chondrocytes. (A) Extracorporeal culture and identification of primary articular chondrocytes in rats. (i) The biosynthesis of intracellular glycosaminoglycan stained positive with toluidine blue. (ii) Immunohistochemical staining of collagen.

10). Tocotrienol-Rich Fractions Offer Potential to Suppress Pulmonary Fibrosis Progression. International Journal of Molecular Sciences (PubMed: 36430808) [IF=5.6]

Application: IHC    Species: Rat    Sample: lung

Figure 8 Amelioration of bleomycin-induced PF: Lungs from different groups were collected, and 5-μm thick sections were prepared and used for the assessment of tissue injury; representative images of IHC staining for collagen II in the lungs: (a) original magnification: 100×; (b) original magnification: 400×; n = 8. Note: * represents p < 0.05 in comparison to the control group; # represents p < 0.05 in comparison to the model group.

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