DRD1 Antibody - #DF7097

Fig. 2: The mechanisms of 5-d UMS in inducing depression-like behavior involve RASD2 and DRD2. a The representative images showing RASD2 expression in the NAc (top, scale bar = 200 and 20 μm), and the number of RASD2-expressing neurons in NAcc and NAcs (bottom). N = 4 mice per group. Two-tailed unpaired t-test: NAcc, t(19) = 2.418, p = 0.0258; NAcs, t(20) = 2.591, p = 0.0175. b Representative images showing RASD2 expression in the mPFC (top, scale bar = 200 and 20 μm), and the number of RASD2-expressing neurons in Cg1, PrL, and IL (bottom). N = 3 mice per group. Two-tailed unpaired t-test: Cg1, t(10) = 2.721, p = 0.0215; PrL, t(11) = 0.9488, p = 0.3631; IL, t(11) = 3.407, p = 0.0059. c 5-d UMS caused changes in RASD2 and dopamine-related protein expression in the NAc. N = 4–7 mice. Two-tailed unpaired t-test: RASD2, t(7) = 3.050, p = 0.0186; DRD1, t(9) = 2.315, p = 0.0458; DRD2, t(7) = 2.597, p = 0.0356; DARPP-32, t(8) = 2.313, p = 0.0494; GluA1, t(10) = 1.035, p = 0.3251. d 5-d UMS caused changes in RASD2 and dopamine-related protein expression in the mPFC. N = 4–7 mice. Two-tailed unpaired t-test: RASD2, t(8) = 2.507, p = 0.0365; DRD1, t(10) = 0.4337, p = 0.6737; DRD2, t(8) = 0.9415, p = 0.3740; DARPP-32, t(7) = 2.981, p = 0.0205; GluA1, t(10) = 2.250, p = 0.0482. e Western blot images of a RASD2 immunoprecipitation showing co-immunoprecipitation of RASD2 and DRD2 in the NAc (left). Quantification of DRD2 co-immunoprecipitation with RASD2 immunoprecipitation. Each sample was normalized by its input and then DRD2 was normalized for the amount of RASD2 immunoprecipitated (right). N = 5 mice per group. Two-tailed unpaired t-test: t(8) = 6.616, p = 0.0002. f Schematic of experimental design and timeline. g 5-d UMS reduces the projection of PrL to the NAcc (scale bar, 20 μm). N = 4 mice per group. Two-tailed unpaired t-test: Cg1, t(22) = 1.665, p = 0.1102; PrL, t(22) = 2.202, p = 0.0385; IL, t(22) = 0.4018, p = 0.6917. The data are expressed as mean ± s.e.m. *p 

Figure 4D1R and D2R expression profiles in the mPFC in different rank groups. (A, B) Representative western-blot images of D1Rs and D2Rs. (C, D) Quantitative analysis of D1R and D2R expression in the mPFC in different rank groups. (E, F) qRT-PCR results showing the relative expression of D1R and D2R mRNA in different rank groups. (G, H) Representative melting curves of D1R and D2R expression during qRT-PCR, showing primer availability and PCR product homogeneity (lines without arrows are β-actin). Panels C–E are analyzed with 1-way ANOVA. Panel F was analyzed with the Kruskal–Wallis test. Data are shown as the mean ± SE; n = 6 in each group; *P < 0.05. dmPFC: dorsal part of the medial prefrontal cortex; vmPFC: ventral part of the medial prefrontal cortex.