DRD1 Antibody - #DF7097

Fig. 2: The mechanisms of 5-d UMS in inducing depression-like behavior involve RASD2 and DRD2. a The representative images showing RASD2 expression in the NAc (top, scale bar = 200 and 20 μm), and the number of RASD2-expressing neurons in NAcc and NAcs (bottom). N = 4 mice per group. Two-tailed unpaired t-test: NAcc, t(19) = 2.418, p = 0.0258; NAcs, t(20) = 2.591, p = 0.0175. b Representative images showing RASD2 expression in the mPFC (top, scale bar = 200 and 20 μm), and the number of RASD2-expressing neurons in Cg1, PrL, and IL (bottom). N = 3 mice per group. Two-tailed unpaired t-test: Cg1, t(10) = 2.721, p = 0.0215; PrL, t(11) = 0.9488, p = 0.3631; IL, t(11) = 3.407, p = 0.0059. c 5-d UMS caused changes in RASD2 and dopamine-related protein expression in the NAc. N = 4–7 mice. Two-tailed unpaired t-test: RASD2, t(7) = 3.050, p = 0.0186; DRD1, t(9) = 2.315, p = 0.0458; DRD2, t(7) = 2.597, p = 0.0356; DARPP-32, t(8) = 2.313, p = 0.0494; GluA1, t(10) = 1.035, p = 0.3251. d 5-d UMS caused changes in RASD2 and dopamine-related protein expression in the mPFC. N = 4–7 mice. Two-tailed unpaired t-test: RASD2, t(8) = 2.507, p = 0.0365; DRD1, t(10) = 0.4337, p = 0.6737; DRD2, t(8) = 0.9415, p = 0.3740; DARPP-32, t(7) = 2.981, p = 0.0205; GluA1, t(10) = 2.250, p = 0.0482. e Western blot images of a RASD2 immunoprecipitation showing co-immunoprecipitation of RASD2 and DRD2 in the NAc (left). Quantification of DRD2 co-immunoprecipitation with RASD2 immunoprecipitation. Each sample was normalized by its input and then DRD2 was normalized for the amount of RASD2 immunoprecipitated (right). N = 5 mice per group. Two-tailed unpaired t-test: t(8) = 6.616, p = 0.0002. f Schematic of experimental design and timeline. g 5-d UMS reduces the projection of PrL to the NAcc (scale bar, 20 μm). N = 4 mice per group. Two-tailed unpaired t-test: Cg1, t(22) = 1.665, p = 0.1102; PrL, t(22) = 2.202, p = 0.0385; IL, t(22) = 0.4018, p = 0.6917. The data are expressed as mean ± s.e.m. *p 

Fig. 3. The effect of TCS on dopaminergic associated mRNA and proteins in vivo. (A) The DA levels in PFC of long-life TCS exposed model rats. (B) Illustration of protein bands of TH, DDC, VMAT2, DRD1, DRD2 and DAT. (C) The relative mRNA and protein expression levels of TH. (D) The relative mRNA and protein expression levels of DDC. (E) The relative mRNA and protein expression levels of VMAT2. (F) The relative mRNA and protein expression levels of DRD1. (G) The relative mRNA and protein expression levels of DRD2. (H) The relative mRNA and protein expression levels of DAT. n = 6.

Figure 4D1R and D2R expression profiles in the mPFC in different rank groups. (A, B) Representative western-blot images of D1Rs and D2Rs. (C, D) Quantitative analysis of D1R and D2R expression in the mPFC in different rank groups. (E, F) qRT-PCR results showing the relative expression of D1R and D2R mRNA in different rank groups. (G, H) Representative melting curves of D1R and D2R expression during qRT-PCR, showing primer availability and PCR product homogeneity (lines without arrows are β-actin). Panels C–E are analyzed with 1-way ANOVA. Panel F was analyzed with the Kruskal–Wallis test. Data are shown as the mean ± SE; n = 6 in each group; *P < 0.05. dmPFC: dorsal part of the medial prefrontal cortex; vmPFC: ventral part of the medial prefrontal cortex.