CX3CR1 Antibody - #DF7096

Figure 2. mRNA and protein expression of CX3CL1 and CX3CR1 in OSCC cell lines. Notes: (A) and (B) show the mRNA expression levels of CX3CL1 and CX3CR1 in HOK and various OSCC cell lines using PCR; (C) and (D) show the protein expression levels of membrane-bound CX3CL1 and CX3CR1 in HOK and various OSCC cell lines using WB, along with their corresponding gray value analysis; (E) shows the OD value analysis of soluble CX3CL1 protein expression in HOK and various OSCC cell lines using ELISA. The results indicate that CX3CL1 mRNA and protein expression levels are significantly decreased in most OSCC cell lines compared to HOK, while soluble CX3CL1 that has the function of chemoattracting cells expressing CX3CR1. Expression is increased and CX3CR1 protein content is significantly decrease. Data were shown as mean with SD (n ≥ 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns p > 0.05. The inconsistent results between PCR and WB may be due to differences in transcription and translation control. The low expression levels of CX3CL1 and CX3CR1 genes in OSCC, as validated by mRNA and protein content analysis in OSCC tissues and cell lines, suggest that they may play a suppressive role in the development and progression of OSCC.

Figure 4. Hepatocyte MagL promotes inflammation resolution after LIR by affecting macrophage M2 polarization The mice in the siMagL group and the siCtrl group were subjected to partial warm ischemia for 90 minutes, and the ischemic lobes of the mice were harvested after 3 days of reperfusion. A NPCs were sorted, and the expressions of Ly6C, CD11b, CX3CR1 and their corresponding quantification were analyzed by flow cytometry. B Representative immunofluorescence images of CD206 (red) and Mertk (green) in ischemic lobes of the liver, Scale bar, 100mm. C Representative immunofluorescence images of Arg1 (red), CX3CR1 (green) and TGF-β (pink) in the ischemic lobe of the liver, Scale bar, 100mm. D Detection of M2 polarization-related markers and cytokine gene expression in liver ischemic lobes by qPCR. E The protein levels of M2 polarization-related markers in ischemic lobes of liver were detected by Western Blot. Data are presented as mean ± SD. ( *, P < 0.05; * *, P < 0.01). The data represent three separate experiments.