Caspase 9 Antibody - #DF7048

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产品: Caspase 9 抗体
货号: DF7048
描述: Rabbit polyclonal antibody to Caspase 9
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep
蛋白号: P55211
RRID: AB_2839004

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Caspase 9 Antibody detects endogenous levels of total Caspase 9.
RRID:
AB_2839004
引用格式: Affinity Biosciences Cat# DF7048, RRID:AB_2839004.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

APAF-3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP-9; CASP9; CASP9_HUMAN; Caspase 9 apoptosis related cysteine peptidase; Caspase 9 Dominant Negative; Caspase 9c; Caspase-9; Caspase-9 subunit p10; ICE LAP6; ICE like apoptotic protease 6; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1, regulatory subunit 56; RNCASP9;

抗原和靶标

免疫原:

A synthesized peptide derived from human Caspase 9, corresponding to a region within the internal amino acids.

基因/基因ID:
CASP9(842)
描述:
Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

研究领域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). Epiberberine induced p53/p21-dependent G2/M cell cycle arrest and cell apoptosis in gastric cancer cells by activating γ-aminobutyric acid receptor- β3. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38006806) [IF=6.7]
2). Targeting CD161 inhibitory receptors enhances NK cell-mediated changes in synovial fibroblast function in rheumatoid arthritis. International immunopharmacology, 2025 (PubMed: 40945032) [IF=4.8]

Application: WB    Species: human    Sample: NK cells

Fig. 5. IFN-γ could mediate FLS functional changes of CIA by activating the PI3K-AKT pathway. (A) Enrichment results of differential gene pathways after IFN-γ stimulation in FLS of CIA. (B) PI3K cascade in GSEA of differentially expressed genes after IFN-γ stimulation of FLS. (C) Expression of STAT3 and PI3K between model and high-IFN-γ treated FLS. (D,E) Transcription and translation of PI3K-AKT and JAK1-STAT3 pathways in different groups of FLS. (F,G) FLS proliferation and apoptosis activity before and after PI3Ki treatment. (H,I) The invasion and metastasis function of FLS before and after PI3Ki treatment. (J,K) Transcription and translation of apoptosis related proteins in different groups of FLS.
3). Baicalin extracted from Huangqin (Radix Scutellariae Baicalensis) induces apoptosis in gastric cancer cells by regulating B cell lymphoma(Bcl-2)/Bcl-2-associated X protein and activating caspase-3 and caspase-9. Journal of Traditional Chinese Medicine, 2017 (PubMed: 29960296) [IF=2.0]

Application: WB    Species: human    Sample: MGC-803 cells

Figure 4| Western blotting analysis of Bcl-2, Bax, caspase-3,and caspase-9 protein expression in MGC-803 and BGC-823 cell lines.A: Bcl-2, Bax, caspase-3, caspase-9 and GAPDH protein expression in BGC-823 cells treated with 80, 120, and 160 μmol/L baicalin for 48 h and in untreated (control) cells.

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