(39)
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产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
DRP1 Antibody detects endogenous levels of total DRP1.
RRID:
AB_2838993
引用格式: Affinity Biosciences Cat# DF7037, RRID:AB_2838993.
引用格式: Affinity Biosciences Cat# DF7037, RRID:AB_2838993.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
DLP1; dnm1l; DNM1L_HUMAN; Dnm1p/Vps1p-like protein; dnml1; DRP1; DVLP; Dymple; Dynamin 1 like; Dynamin family member proline-rich carboxyl-terminal domain less; Dynamin like protein; Dynamin related protein 1; Dynamin-1-like protein; Dynamin-like protein 4; Dynamin-like protein; Dynamin-like protein IV; Dynamin-related protein 1; DYNIV 11; EMPF; EMPF1; FLJ41912; HdynIV; VPS1;
抗原和靶标
免疫原:
A synthesized peptide derived from human DRP1, corresponding to a region within C-terminal amino acids.
基因/基因ID:
描述:
Dynamin-related protein 1 (DRP1) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1). DRP1 affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5). The yeast ortholog of DRP1 clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3). The division of the mitochondria, which is required for apoptosis, as well as normal cell growth and development is controlled, in part, by the phosphorylation of DRP1 at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6). When phosphorylated at Ser616, DRP1 stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DRP1 is phosphorylated at Ser637 (reviewed in 6). Dephosphorylation at Ser637 by calcineurin reverses this inhibition (7). In addition to phosphorylation, sumoylation of DRP1 is also an enhancer of mitochondrial fission (8). Balancing fission and fusion events is essential for proper mitochondrial function. Research studies have demonstrated mitochondrial defects in a variety of neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease (reviewed in 6).
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
文献引用
1). CircHIPK3 targets DRP1 to mediate hydrogen peroxide-induced necroptosis of vascular smooth muscle cells and atherosclerotic vulnerable plaque formation. Journal of advanced research, 2025
(PubMed: 38621622)
[IF=11.4]
2). HSP70 attenuates compression-induced apoptosis of nucleus pulposus cells by suppressing mitochondrial fission via upregulating the expression of SIRT3. EXPERIMENTAL AND MOLECULAR MEDICINE, 2022
(PubMed: 35338257)
[IF=9.5]
Application: IF/ICC Species: Rat Sample: NP cells
Fig. 2
HSP70 suppressed compression-induced apoptosis of NP cells.
a, b The effects of TRC on apoptosis of NP cells measured by flow cytometry using Annexin V-FITC/PI staining. c Typical fluorescence photomicrograph of TUNEL staining of NP cells (scale bar: 50 μm). d, e The effects of VER on apoptosis of NP cells measured by flow cytometry using Annexin V-FITC/PI staining. f Typical fluorescence photomicrograph of TUNEL staining of NP cells (scale bar: 50 μm). (N = 3, **P < 0.01; ***P < 0.001).
Application: WB Species: Rat Sample: NP cells
Fig. 5
HSP70 suppressed compression-induced mitochondrial fission in NP cells.
a The effects of TRC on the expression of DRP1, MFF, Fis1, Mfn1, Mfn2, and OPA1 (N = 3). b Representative fluorescence photomicrograph of TOM20 examined by immunofluorescence staining (N = 3, original magnification: ×1000). c The morphological ultrastructural appearance of mitochondria observed by TEM (N = 3). d The effects of VER on the expression of DRP1, MFF, Fis1, Mfn1, Mfn2, and OPA1 in NP cells treated with TRC (N = 3). e Representative fluorescence photomicrograph of TOM20 examined by immunofluorescence staining of NP cells treated with TRC and VER (N = 3, original magnification: ×1000). f Representative fluorescence photomicrograph of TOM20 examined by immunofluorescence staining of NP cells treated with TRC and HSP70-specific siRNAs (N = 3, original magnification: ×1000).
3). YAP1 inhibits the senescence of alveolar epithelial cells by targeting Prdx3 to alleviate pulmonary fibrosis. Experimental & molecular medicine, 2024
(PubMed: 38945958)
[IF=9.5]
4). USP7 promotes cardiometabolic disorders and mitochondrial homeostasis dysfunction in diabetic mice via stabilizing PGC1β. Pharmacological research, 2024
(PubMed: 38815879)
[IF=9.1]
Application: WB Species: Mouse Sample: heart
Fig. 6. USP7 inhibition improved cardiac mitochondrial dynamics in diabetic mice and NMCMs treated with HG+PA or H9c2 cells treated with PA. (A) Representative Western blot and quantification of Drp1, Fsi1, Mfn2 and Opa1 expression in the hearts of diabetic mice treated with P5091. n = 5 in each group. *P
5). Increased TSPO alleviates neuropathic pain by preventing pyroptosis via the AMPK-PGC-1α pathway. The journal of headache and pain, 2025
(PubMed: 39871133)
[IF=7.3]
6). Zinc ions facilitate metabolic bioenergetic recovery post spinal cord injury by activating microglial mitophagy through the STAT3-FOXO3a-SOD2 pathway. Free radical biology & medicine, 2024
(PubMed: 39613048)
[IF=7.1]
7). The Chinese herbal medicine Fufang Zhenzhu Tiaozhi ameliorates diabetic cardiomyopathy by regulating cardiac abnormal lipid metabolism and mitochondrial dynamics in diabetic mice. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023
(PubMed: 37302318)
[IF=6.9]
8). HSP70 alleviates sepsis-induced cardiomyopathy by attenuating mitochondrial dysfunction-initiated NLRP3 inflammasome-mediated pyroptosis in cardiomyocytes. Burns & trauma, 2022
(PubMed: 36439706)
[IF=6.3]
9). FGFR2 Mutation p.Cys342Arg Enhances Mitochondrial Metabolism-Mediated Osteogenesis via FGF/FGFR-AMPK-Erk1/2 Axis in Crouzon Syndrome. Cells, 2022
(PubMed: 36231091)
[IF=6.0]
Application: WB Species: Human Sample:
Figure 7
Mitochondrial dynamics participate in osteogenesis mediated by FGFR/FGFR2-AMPK pathway: (A) Mito-tracker red and mitochondria 2D analysis showed more fragmented or punctate mitochondria with fewer and shorter branches in the MT group, while there were widely reticular mitochondria with longer branches in the WT group. (B) qRT-PCR and Western blot analysis demonstrated that the expressions of mitochondrial-fusion-related factors Mfn2 and Opa1 were downregulated, however, the expression of mitochondrial-fission-related factor Drp1 was upregulated in the MT group compared to the WT group. (C) Mito-tracker red and mitochondria 2D analysis showed a filamentous network of mitochondria with more and longer branches widely spread in the cytoplasm in the siFGFR2 group. (D) qPCR and Western blot analysis showed that the expression of fusion-related genes Mfn2 and Opa1 was increased and the expression of fission-related gene Drp1 was decreased in the siFGFR2 group. (E) After treatment with Compound C, the morphology of the mitochondria which should have tended to split adopted a fused reticular structure with more and longer branches, as shown by Mito-tracker red and mitochondria 2D analysis. (F) Western blot analysis showed the level of MFN2 and OPA1 was increased and then level of DRP1 was decreased, as induced by Compound C. p values were significant at * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.
10). Silibinin Induces G2/M Cell Cycle Arrest by Activating Drp1-Dependent Mitochondrial Fission in Cervical Cancer. Frontiers in Pharmacology, 2020
(PubMed: 32226384)
[IF=5.6]
Application: WB Species: human Sample: cervical cancer cells
FIGURE 6 | SB impaired mitochondrial fission in cervical cancer cells. (B) The mitochondrial fission related protein expression extracted from cervical cancer cells. Drp1 expression was increased in both two cervical cancer cells. The mitochondrial fission related protein and gene expression level in cervical cancer cells were detected by western blotting and qPCR, respectively. Values (mean ± SDs) were obtained from at least three independent experiments.*P < 0.05, **P < 0.01, and ***P < 0.001 by one-way ANOVA with Tukey’s test.
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